AHRR ORF clone FHC00775

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KOP HaloTag ORF List Flexi search

Halotag ORF Clone FHC00775

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Clone info.

Species

human

Clone Name

pFN21ASDA1234 Sequence (with vector) Restriction Map

Product ID

FHC00775

Flexi Clone PID

FXC00775

ORF Sequence

DNA sequence (2157 bp)
Protein sequence (719 aa)

Linker Type

Flexi_RBS type

more Linker info

Vector Type

pFN21A Vector sequence

Restriction Map

Cloning Site

SgfI-PmeI

GenBank Accession

ORF sequence is derived from [AB384149]

Gene

KIAA1234

Symbol

AHRR

Alias : AHH, AHHR, bHLHe77

Description

aryl-hydrocarbon receptor repressor, transcript variant 1

Original Clone ID

fh08618

Quality control data of this clone

Description

Characterization of the cloned ORF

5'-end(FN21F1)

3'-end(F1KR)

Agarose gel electrophoretic image

5'/3'-end sequences (chromatogram)

N-terminal HaloTag-fusions expressed in HEK293 visualized by HaloTag TMR ligand

Cell image

SDS-PAGE

Features of the cloned ORF sequence

Description

Length: 2157 bp
Restriction map A Restriction map B
Genomic Structure

Features of the protein sequence

Description

Length: 719 aa
Result of homology search against CCDS protein database ( FASTA output, Multiple alignment) Entry Exp ID% symbol CCDS43297.1 5.7e-186 99.9 AHRR CCDS56355.1 6.6e-119 97.4 AHRR CCDS5366.1 2.4e-26 42.1 AHR The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.
Result of motif / domain search (InterProScan and SOSUI) [ zoom out (x2) ]
Result of InterProScan Search method interpro_ID From To Entry Definition HMMPfam IPR001092 38 79 PF00010 Helix-loop-helix DNA-binding domain IPR013767 116 179 PF00989 PAS fold HMMSmart IPR001092 35 89 SM00353 Helix-loop-helix DNA-binding domain IPR000014 114 180 SM00091 PAS ProfileScan IPR001092 17 82 PS50888 Helix-loop-helix DNA-binding domain IPR000014 117 182 PS50112 PAS
Result of homology search against disease genes in OMIM database(FASTA output, Multiple alignment) H_id H_len (aa) OMIM_No. ID (%) Q_ali/Q_len (%) NP_065782 719 606517 99.9 100.0 NP_001229341 701 606517 97.4 100.0 Inquiries or Suggestions ? Send a message to flexiclone AT kazusagt.com

ORF DNA

Cloned ORF Nucleotide sequence for pFN21ASDA1234 Download

>KIAA1234  2157 bp
ATGCCGAGGACGATGATCCCGCCGGGGGAGTGCACGTACGCGGGCCGGAAGCGGAGGAGG
CCCCTGCAGAAACAGAGGCCCGCCGTGGGGGCAGAGAAGTCCAACCCCTCCAAGCGACAC
CGGGACCGCCTCAACGCCGAGTTGGACCACCTGGCCAGCCTGCTGCCGTTCCCGCCTGAC
ATCATCTCCAAGCTGGACAAGCTTTCTGTCCTGCGCCTCAGTGTCAGTTACCTCCGGGTG
AAGAGCTTCTTCCAAGTCGTGCAGGAGCAGAGCTCACGGCAGCCTGCGGCCGGCGCCCCC
TCGCCCGGAGACAGCTGTCCTCTTGCAGGGTCTGCCGTGCTGGAGGGAAGGCTGCTGTTG
GAGTCTCTTAATGGCTTTGCTCTGGTCGTGAGTGCAGAAGGGACGATATTTTATGCATCA
GCAACGATCGTGGACTATCTGGGCTTCCATCAGACGGATGTAATGCACCAGAACATTTAT
GACTACATCCACGTGGACGACCGCCAGGACTTCTGCCGGCAGCTCCACTGGGCCATGGAC
CCTCCCCAGGTGGTGTTTGGGCAGGCCCCGCCCTTGGAGACAGGAGATGATGCTATCCTG
GGGAGGCTGCTCAGGGCCCAGGAGTGGGGCACAGGCACGCCCACCGAGTACTCGGCCTTC
CTGACCCGCTGCTTCATCTGCCGTGTGCGCTGCCTGCTGGACAGCACCTCGGGCTTCCTG
GCCCGGGGGTCACAGGCTTGGCAGCTGCGGCTCTGCTGTCCCGAGCCACTCATGACGATG
CAGTTTCAAGGAAAACTAAAATTCCTGTTTGGACAGAAGAAGAAGGCGCCGTCAGGAGCC
ATGCTCCCGCCGCGGCTGTCGCTGTTCTGCATTGCGGCACCCGTTCTCCTCCCCTCCGCA
GCGGAGATGAAAATGAGGAGCGCGCTCCTGAGGGCAAAACCCAGAGCAGACACCGCAGCC
ACCGCGGATGCAAAAGTAAAAGCCACCACCAGTCTGTGCGAATCGGAACTGCATGGAAAA
CCCAATTACTCAGCAGGAAGGAGCAGCAGAGAGAGCGGCGTTTTGGTGCTCAGGGAACAG
ACTGACGCTGGCCGATGGGCACAGGTTCCCGCCAGGGCCCCATGCCTGTGCCTCCGGGGT
GGCCCTGACCTTGTCCTTGACCCCAAGGGGGGCTCAGGGGACAGGGAGGAGGAGCAGCAC
AGGATGCTGAGCAGGGCCTCTGGAGTGACAGGGCGGAGGGAGACTCCAGGACCCACAAAG
CCCCTGCCCTGGACAGCGGGAAAGCACAGTGAGGATGGTGCCAGGCCGAGGCTGCAGCCC
AGCAAGAATGACCCGCCCTCCCTGCGCCCCATGCCCCGCGGCTCCTGCCTGCCCTGCCCG
TGTGTCCAGGGCACTTTCAGGAACTCGCCCATCTCTCACCCGCCGAGCCCGTCCCCCAGT
GCCTACTCCAGCCGGACCAGCAGACCCATGCGGGATGTCGGTGAGGACCAGGTGCACCCT
CCCCTCTGCCACTTTCCCCAGAGGAGCCTGCAGCACCAGCTCCCTCAGCCTGGAGCTCAG
CGTTTTGCCACGAGGGGCTATCCCATGGAGGACATGAAGCTGCAAGGTGTACCGATGCCT
CCGGGGGACCTGTGTGGTCCGACGCTGCTGCTAGATGTGTCCATCAAGATGGAGAAGGAC
TCTGGGTGTGAGGGTGCTGCAGACGGCTGTGTGCCCAGCCAGGTGTGGCTGGGGGCCAGT
GACAGGAGCCACCCAGCCACCTTCCCTACCAGGATGCACCTGAAAACAGAGCCAGACTCT
CGGCAACAGGTGTACATCTCGCACCTGGGGCACGGCGTGCGGGGGGCTCAGCCCCATGGG
AGGGCCACTGCTGGGCGCAGCAGGGAGCTGACCCCTTTCCACCCTGCACACTGTGCCTGC
CTGGAGCCCACAGACGGCCTTCCCCAGTCGGAGCCTCCCCACCAGCTCTGTGCACGGGGC
CGAGGTGAACAGTCCTGCACCTGCAGAGCTGCTGAGGCCGCCCCTGTGGTCAAGCGGGAG
CCCTTGGACTCACCCCAGTGGGCTACTCACAGCCAGGGAATGGTGCCCGGGATGTTGCCC
AAAAGTGCCTTGGCCACGCTGGTCCCGCCCCAAGCTTCGGGGTGCACATTCCTGCCA

ORF Protein

Cloned ORF protein sequence for pFN21ASDA1234 Download

>KIAA1234  719 aa
MPRTMIPPGECTYAGRKRRRPLQKQRPAVGAEKSNPSKRHRDRLNAELDHLASLLPFPPD
IISKLDKLSVLRLSVSYLRVKSFFQVVQEQSSRQPAAGAPSPGDSCPLAGSAVLEGRLLL
ESLNGFALVVSAEGTIFYASATIVDYLGFHQTDVMHQNIYDYIHVDDRQDFCRQLHWAMD
PPQVVFGQAPPLETGDDAILGRLLRAQEWGTGTPTEYSAFLTRCFICRVRCLLDSTSGFL
ARGSQAWQLRLCCPEPLMTMQFQGKLKFLFGQKKKAPSGAMLPPRLSLFCIAAPVLLPSA
AEMKMRSALLRAKPRADTAATADAKVKATTSLCESELHGKPNYSAGRSSRESGVLVLREQ
TDAGRWAQVPARAPCLCLRGGPDLVLDPKGGSGDREEEQHRMLSRASGVTGRRETPGPTK
PLPWTAGKHSEDGARPRLQPSKNDPPSLRPMPRGSCLPCPCVQGTFRNSPISHPPSPSPS
AYSSRTSRPMRDVGEDQVHPPLCHFPQRSLQHQLPQPGAQRFATRGYPMEDMKLQGVPMP
PGDLCGPTLLLDVSIKMEKDSGCEGAADGCVPSQVWLGASDRSHPATFPTRMHLKTEPDS
RQQVYISHLGHGVRGAQPHGRATAGRSRELTPFHPAHCACLEPTDGLPQSEPPHQLCARG
RGEQSCTCRAAEAAPVVKREPLDSPQWATHSQGMVPGMLPKSALATLVPPQASGCTFLP

ORF+Vector

Nucleotide Sequence (with vector) for pFN21ASDA1234 Download

>pFN21ASDA1234  6886 bp
TCAATATTGGCCATTAGCCATATTATTCATTGGTTATATAGCATAAATCAATATTGGCTA
TTGGCCATTGCATACGTTGTATCTATATCATAATATGTACATTTATATTGGCTCATGTCC
AATATGACCGCCATGTTGGCATTGATTATTGACTAGTTATTAATAGTAATCAATTACGGG
GTCATTAGTTCATAGCCCATATATGGAGTTCCGCGTTACATAACTTACGGTAAATGGCCC
GCCTGGCTGACCGCCCAACGACCCCCGCCCATTGACGTCAATAATGACGTATGTTCCCAT
AGTAACGCCAATAGGGACTTTCCATTGACGTCAATGGGTGGAGTATTTACGGTAAACTGC
CCACTTGGCAGTACATCAAGTGTATCATATGCCAAGTCCGCCCCCTATTGACGTCAATGA
CGGTAAATGGCCCGCCTGGCATTATGCCCAGTACATGACCTTACGGGACTTTCCTACTTG
GCAGTACATCTACGTATTAGTCATCGCTATTACCATGGTGATGCGGTTTTGGCAGTACAC
CAATGGGCGTGGATAGCGGTTTGACTCACGGGGATTTCCAAGTCTCCACCCCATTGACGT
CAATGGGAGTTTGTTTTGGCACCAAAATCAACGGGACTTTCCAAAATGTCGTAATAACCC
CGCCCCGTTGACGCAAATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGC
TGGTTTAGTGAACCGTCAGATCACTAGAAGCTTTATTGCGGTAGTTTATCACAGTTAAAT
TGCTAACGCAGTCAGTGCTTCTGACACAACAGTCTCGAACTTAAGCTGCAGAAGTTGGTC
GTGAGGCACTGGGCAGGTAAGTATCAAGGTTACAAGACAGGTTTAAGGAGACCAATAGAA
ACTGGGCTTGTCGAGACAGAGAAGACTCTTGCGTTTCTGATAGGCACCTATTGGTCTTAC
TGACATCCACTTTGCCTTTCTCTCCACAGGTGTCCACTCCCAGTTCAATTACAGCTCTTA
AGGCTAGAGTATTAATACGACTCACTATAGGGCTAGCAAAGCCACCATGGCAGAAATCGG
TACTGGCTTTCCATTCGACCCCCATTATGTGGAAGTCCTGGGCGAGCGCATGCACTACGT
CGATGTTGGTCCGCGCGATGGCACCCCTGTGCTGTTCCTGCACGGTAACCCGACCTCCTC
CTACGTGTGGCGCAACATCATCCCGCATGTTGCACCGACCCATCGCTGCATTGCTCCAGA
CCTGATCGGTATGGGCAAATCCGACAAACCAGACCTGGGTTATTTCTTCGACGACCACGT
CCGCTTCATGGATGCCTTCATCGAAGCCCTGGGTCTGGAAGAGGTCGTCCTGGTCATTCA
CGACTGGGGCTCCGCTCTGGGTTTCCACTGGGCCAAGCGCAATCCAGAGCGCGTCAAAGG
TATTGCATTTATGGAGTTCATCCGCCCTATCCCGACCTGGGACGAATGGCCAGAATTTGC
CCGCGAGACCTTCCAGGCCTTCCGCACCACCGACGTCGGCCGCAAGCTGATCATCGATCA
GAACGTTTTTATCGAGGGTACGCTGCCGATGGGTGTCGTCCGCCCGCTGACTGAAGTCGA
GATGGACCATTACCGCGAGCCGTTCCTGAATCCTGTTGACCGCGAGCCACTGTGGCGCTT
CCCAAACGAGCTGCCAATCGCCGGTGAGCCAGCGAACATCGTCGCGCTGGTCGAAGAATA
CATGGACTGGCTGCACCAGTCCCCTGTCCCGAAGCTGCTGTTCTGGGGCACCCCAGGCGT
TCTGATCCCACCGGCCGAAGCCGCTCGCCTGGCCAAAAGCCTGCCTAACTGCAAGGCTGT
GGACATCGGCCCGGGTCTGAATCTGCTGCAAGAAGACAACCCGGACCTGATCGGCAGCGA
GATCGCGCGCTGGCTGTCGACGCTCGAGATTTCCGGCGAGCCAACCACTGAGGATCTGTA
CTTTCAGAGCGATAACGCGATCGCTTTCGAAGGAGATAGAACCATGCCGAGGACGATGAT
CCCGCCGGGGGAGTGCACGTACGCGGGCCGGAAGCGGAGGAGGCCCCTGCAGAAACAGAG
GCCCGCCGTGGGGGCAGAGAAGTCCAACCCCTCCAAGCGACACCGGGACCGCCTCAACGC
CGAGTTGGACCACCTGGCCAGCCTGCTGCCGTTCCCGCCTGACATCATCTCCAAGCTGGA
CAAGCTTTCTGTCCTGCGCCTCAGTGTCAGTTACCTCCGGGTGAAGAGCTTCTTCCAAGT
CGTGCAGGAGCAGAGCTCACGGCAGCCTGCGGCCGGCGCCCCCTCGCCCGGAGACAGCTG
TCCTCTTGCAGGGTCTGCCGTGCTGGAGGGAAGGCTGCTGTTGGAGTCTCTTAATGGCTT
TGCTCTGGTCGTGAGTGCAGAAGGGACGATATTTTATGCATCAGCAACGATCGTGGACTA
TCTGGGCTTCCATCAGACGGATGTAATGCACCAGAACATTTATGACTACATCCACGTGGA
CGACCGCCAGGACTTCTGCCGGCAGCTCCACTGGGCCATGGACCCTCCCCAGGTGGTGTT
TGGGCAGGCCCCGCCCTTGGAGACAGGAGATGATGCTATCCTGGGGAGGCTGCTCAGGGC
CCAGGAGTGGGGCACAGGCACGCCCACCGAGTACTCGGCCTTCCTGACCCGCTGCTTCAT
CTGCCGTGTGCGCTGCCTGCTGGACAGCACCTCGGGCTTCCTGGCCCGGGGGTCACAGGC
TTGGCAGCTGCGGCTCTGCTGTCCCGAGCCACTCATGACGATGCAGTTTCAAGGAAAACT
AAAATTCCTGTTTGGACAGAAGAAGAAGGCGCCGTCAGGAGCCATGCTCCCGCCGCGGCT
GTCGCTGTTCTGCATTGCGGCACCCGTTCTCCTCCCCTCCGCAGCGGAGATGAAAATGAG
GAGCGCGCTCCTGAGGGCAAAACCCAGAGCAGACACCGCAGCCACCGCGGATGCAAAAGT
AAAAGCCACCACCAGTCTGTGCGAATCGGAACTGCATGGAAAACCCAATTACTCAGCAGG
AAGGAGCAGCAGAGAGAGCGGCGTTTTGGTGCTCAGGGAACAGACTGACGCTGGCCGATG
GGCACAGGTTCCCGCCAGGGCCCCATGCCTGTGCCTCCGGGGTGGCCCTGACCTTGTCCT
TGACCCCAAGGGGGGCTCAGGGGACAGGGAGGAGGAGCAGCACAGGATGCTGAGCAGGGC
CTCTGGAGTGACAGGGCGGAGGGAGACTCCAGGACCCACAAAGCCCCTGCCCTGGACAGC
GGGAAAGCACAGTGAGGATGGTGCCAGGCCGAGGCTGCAGCCCAGCAAGAATGACCCGCC
CTCCCTGCGCCCCATGCCCCGCGGCTCCTGCCTGCCCTGCCCGTGTGTCCAGGGCACTTT
CAGGAACTCGCCCATCTCTCACCCGCCGAGCCCGTCCCCCAGTGCCTACTCCAGCCGGAC
CAGCAGACCCATGCGGGATGTCGGTGAGGACCAGGTGCACCCTCCCCTCTGCCACTTTCC
CCAGAGGAGCCTGCAGCACCAGCTCCCTCAGCCTGGAGCTCAGCGTTTTGCCACGAGGGG
CTATCCCATGGAGGACATGAAGCTGCAAGGTGTACCGATGCCTCCGGGGGACCTGTGTGG
TCCGACGCTGCTGCTAGATGTGTCCATCAAGATGGAGAAGGACTCTGGGTGTGAGGGTGC
TGCAGACGGCTGTGTGCCCAGCCAGGTGTGGCTGGGGGCCAGTGACAGGAGCCACCCAGC
CACCTTCCCTACCAGGATGCACCTGAAAACAGAGCCAGACTCTCGGCAACAGGTGTACAT
CTCGCACCTGGGGCACGGCGTGCGGGGGGCTCAGCCCCATGGGAGGGCCACTGCTGGGCG
CAGCAGGGAGCTGACCCCTTTCCACCCTGCACACTGTGCCTGCCTGGAGCCCACAGACGG
CCTTCCCCAGTCGGAGCCTCCCCACCAGCTCTGTGCACGGGGCCGAGGTGAACAGTCCTG
CACCTGCAGAGCTGCTGAGGCCGCCCCTGTGGTCAAGCGGGAGCCCTTGGACTCACCCCA
GTGGGCTACTCACAGCCAGGGAATGGTGCCCGGGATGTTGCCCAAAAGTGCCTTGGCCAC
GCTGGTCCCGCCCCAAGCTTCGGGGTGCACATTCCTGCCATACGTAGTTTAAACGAATTC
GGGCTCGGTACCCGGGGATCCTCTAGAGTCGACCTGCAGGCATGCAAGCTGATCCGGCTG
CTAACAAAGCCCGAAAGGAAGCTGAGTTGGCTGCTGCCACCGCTGAGCAATAACTAGCAT
AACCCCTTGGGGCGGCCGCTTCGAGCAGACATGATAAGATACATTGATGAGTTTGGACAA
ACCACAACTAGAATGCAGTGAAAAAAATGCTTTATTTGTGAAATTTGTGATGCTATTGCT
TTATTTGTAACCATTATAAGCTGCAATAAACAAGTTAACAACAACAATTGCATTCATTTT
ATGTTTCAGGTTCAGGGGGAGATGTGGGAGGTTTTTTTAAGCAAGTAAAACCTCTACAAA
TGTGGTAAAATCGAATTCTAATGGATCCTCTTTGCGCTTGCGTTTTCCCTTGTCCAGATA
GCCCAGTAGCTGACATTCATCCGGGGTCAGCACCGTTTCTGCGGACTGGCTTTCTACGTA
ATGGTTTCTTAGACGTCAGGTGGCACTTTTCGGGGAAATGTGCGCGGAACCCCTATTTGT
TTATTTTTCTAAATACATTCAAATATGTATCCGCTCATGAGACAATAACCCTGATAAATG
CTTCAATAATATTGAAAAAGGAAGAGTATGAGTATTCAACATTTCCGTGTCGCCCTTATT
CCCTTTTTTGCGGCATTTTGCCTTCCTGTTTTTGCTCACCCAGAAACGCTGGTGAAAGTA
AAAGATGCTGAAGATCAGTTGGGTGCACGAGTGGGTTACATCGAACTGGATCTCAACAGC
GGTAAGATCCTTGAGAGTTTTCGCCCCGAAGAACGTTTTCCAATGATGAGCACTTTCAAA
GTTCTGCTATGTGGCGCGGTATTATCCCGTATTGACGCCGGGCAAGAGCAACTCGGTCGC
CGCATACACTATTCTCAGAATGACTTGGTTGAGTACTCACCAGTCACAGAAAAGCATCTT
ACGGATGGCATGACAGTAAGAGAATTATGCAGTGCTGCCATAACCATGAGTGATAACACT
GCGGCCAACTTACTTCTGACAACTATCGGAGGACCGAAGGAGCTAACCGCTTTTTTGCAC
AACATGGGGGATCATGTAACTCGCCTTGATCGTTGGGAACCGGAGCTGAATGAAGCCATA
CCAAACGACGAGCGTGACACCACGATGCCTGTAGCAATGGCAACAACGTTGCGCAAACTA
TTAACTGGCGAACTACTTACTCTAGCTTCCCGGCAACAATTAATAGACTGGATGGAGGCG
GATAAAGTTGCAGGACCACTTCTGCGCTCGGCCCTTCCGGCTGGCTGGTTTATTGCTGAT
AAATCTGGAGCCGGTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCAGATGGT
AAGCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGA
AATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAATTCGAAATGACC
GACCAAGCGACGCCCAACCGGTATCAGCTCACTCAAAGGCGGTAATACGGTTATCCACAG
AATCAGGGGATAACGCAGGAAAGAACATGTGAGCAAAAGGCCAGCAAAAGGCCAGGAACC
GTAAAAAGGCCGCGTTGCTGGCGTTTTTCCATAGGCTCCGCCCCCCTGACGAGCATCACA
AAAATCGACGCTCAAGTCAGAGGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGT
TTCCCCCTGGAAGCTCCCTCGTGCGCTCTCCTGTTCCGACCCTGCCGCTTACCGGATACC
TGTCCGCCTTTCTCCCTTCGGGAAGCGTGGCGCTTTCTCATAGCTCACGCTGTAGGTATC
TCAGTTCGGTGTAGGTCGTTCGCTCCAAGCTGGGCTGTGTGCACGAACCCCCCGTTCAGC
CCGACCGCTGCGCCTTATCCGGTAACTATCGTCTTGAGTCCAACCCGGTAAGACACGACT
TATCGCCACTGGCAGCAGCCACTGGTAACAGGATTAGCAGAGCGAGGTATGTAGGCGGTG
CTACAGAGTTCTTGAAGTGGTGGCCTAACTACGGCTACACTAGAAGGACAGTATTTGGTA
TCTGCGCTCTGCTGAAGCCAGTTACCTTCGGAAAAAGAGTTGGTAGCTCTTGATCCGGCA
AACAAACCACCGCTGGTAGCGGTGGTTTTTTTGTTTGCAAGCAGCAGATTACGCGCAGAA
AAAAAGGATTTCAAGAAGATCCTTTGATCTTTTCTACGGGGTCTGACGCTCAGTGGAACG
AAAACTCACGTTAAGGGATTTTGGTCATGAGATTATCAAAAAGGATCTTCACCTAGATCC
TTTTATAGTCCGGAAATACAGGAACGCACGCTGGATGGCCCTTCGCTGGGATGGTGAAAC
CATGAAAAATGGCAGCTTCAGTGGATTAAGTGGGGGTAATGTGGCCTGTACCCTCTGGTT
GCATAGGTATTCATACGGTTAAAATTTATCAGGCGCGATTGCGGCAGTTTTTCGGGTGGT
TTGTTGCCATTTTTACCTGTCTGCTGCCGTGATCGCGCTGAACGCGTTTTAGCGGTGCGT
ACAATTAAGGGATTATGGTAAATCCACTTACTGTCTGCCCTCGTAGCCATCGAGATAAAC
CGCAGTACTCCGGCCACGATGCGTCCGGCGTAGAGGATCGAGATCT