SH3GLB1 ORF clone FHC00089

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KOP HaloTag ORF List Flexi search

Halotag ORF Clone FHC00089

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Clone info.

Species

human

Clone Name

pFN21AA0491 Sequence (with vector) Restriction Map

Product ID

FHC00089

Flexi Clone PID

FXC00089

ORF Sequence

DNA sequence (1095 bp)
Protein sequence (365 aa)

Linker Type

Flexi type

more Linker info

Vector Type

pFN21A Vector sequence

Restriction Map

Cloning Site

SgfI-PmeI

GenBank Accession

ORF sequence is derived from [AB385341]

Gene

KIAA0491

Symbol

SH3GLB1

Alias : Bif-1, CGI-61, PPP1R70, dJ612B15.2

Description

SH3-domain GRB2-like endophilin B1, transcript variant 1

Original Clone ID

ha02617

Quality control data of this clone

Description

Characterization of the cloned ORF

5'-end(FN21F1)

3'-end(F1KR)

Agarose gel electrophoretic image

5'/3'-end sequences (chromatogram)

N-terminal HaloTag-fusions expressed in HEK293 visualized by HaloTag TMR ligand

Cell image

SDS-PAGE

Features of the cloned ORF sequence

Description

Length: 1095 bp
Restriction map A Restriction map B
Genomic Structure

Features of the protein sequence

Description

Length: 365 aa
Result of homology search against CCDS protein database ( FASTA output, Multiple alignment) Entry Exp ID% symbol CCDS710.1 9.9e-124 100.0 SH3GLB1 CCDS55613.1 3e-88 100.0 SH3GLB1 CCDS55612.1 1.6e-61 94.6 SH3GLB1 CCDS72819.1 1.6e-61 92.6 SH3GLB1 CCDS69680.1 4.9e-44 58.5 SH3GLB2 The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.
Result of motif / domain search (InterProScan and SOSUI) [ zoom out (x2) ]
Result of InterProScan Search method interpro_ID From To Entry Definition HMMPfam IPR004148 19 253 PF03114 BAR IPR001452 311 357 PF00018 Src homology-3 domain HMMSmart IPR004148 10 254 SM00721 BAR IPR001452 308 365 SM00326 Src homology-3 domain ProfileScan IPR004148 27 261 PS51021 BAR IPR001452 305 365 PS50002 Src homology-3 domain
Result of homology search against disease genes in OMIM database(FASTA output, Multiple alignment) H_id H_len (aa) OMIM_No. ID (%) Q_ali/Q_len (%) NP_057093 365 609287 100.0 100.0 XP_005252157 379 609288 62.9 99.2 XP_011539840 378 609287 96.6 100.0 NP_001193581 386 609287 94.6 100.0 NP_001193580 394 609287 92.6 100.0 Inquiries or Suggestions ? Send a message to flexiclone AT kazusagt.com

ORF DNA

Cloned ORF Nucleotide sequence for pFN21AA0491 Download

>KIAA0491  1095 bp
ATGAATATCATGGACTTCAACGTGAAGAAGCTGGCGGCCGACGCAGGCACCTTCCTCAGT
CGCGCCGTGCAGTTCACAGAAGAAAAGCTTGGCCAGGCTGAGAAGACAGAATTGGATGCT
CACTTAGAGAACCTCCTTAGCAAAGCTGAATGTACCAAAATATGGACAGAAAAAATAATG
AAACAAACTGAAGTGTTATTGCAGCCAAATCCAAATGCCAGGATAGAAGAATTTGTTTAT
GAGAAACTGGATAGAAAAGCTCCAAGTCGTATAAACAACCCAGAACTTTTGGGACAATAT
ATGATTGATGCAGGGACTGAGTTTGGCCCAGGAACAGCTTATGGTAATGCCCTTATTAAA
TGTGGAGAAACCCAAAAAAGAATTGGAACAGCAGACAGAGAACTGATTCAAACGTCAGCC
TTAAATTTTCTTACTCCTTTAAGAAACTTTATAGAAGGAGATTACAAAACAATTGCTAAA
GAAAGGAAACTATTGCAAAATAAGAGACTGGATTTGGATGCTGCAAAAACGAGACTAAAA
AAGGCAAAAGCTGCAGAAACTAGAAATTCATCTGAACAGGAATTAAGAATAACTCAAAGT
GAATTTGATCGTCAAGCAGAGATTACCAGACTTCTGCTAGAGGGAATCAGCAGTACACAT
GCCCATCACCTTCGCTGTCTGAATGACTTTGTAGAAGCCCAGATGACTTACTATGCACAG
TGTTACCAGTATATGTTGGACCTCCAGAAACAACTGGGAAGTTTTCCATCCAATTATCTT
AGTAACAACAATCAGACTTCTGTGACACCTGTACCATCAGTTTTACCAAATGCGATTGGT
TCTTCTGCCATGGCTTCAACAAGTGGCCTAGTAATCACCTCTCCTTCCAACCTCAGTGAC
CTTAAGGAGTGTAGTGGCAGCAGAAAGGCCAGGGTTCTCTATGATTATGATGCAGCAAAC
AGTACTGAATTATCACTTCTGGCAGATGAGGTGATCACTGTGTTCAGTGTTGTTGGAATG
GATTCAGACTGGCTAATGGGGGAAAGGGGAAACCAGAAGGGCAAGGTGCCAATTACCTAC
TTAGAACTGCTCAAT

ORF Protein

Cloned ORF protein sequence for pFN21AA0491 Download

>KIAA0491  365 aa
MNIMDFNVKKLAADAGTFLSRAVQFTEEKLGQAEKTELDAHLENLLSKAECTKIWTEKIM
KQTEVLLQPNPNARIEEFVYEKLDRKAPSRINNPELLGQYMIDAGTEFGPGTAYGNALIK
CGETQKRIGTADRELIQTSALNFLTPLRNFIEGDYKTIAKERKLLQNKRLDLDAAKTRLK
KAKAAETRNSSEQELRITQSEFDRQAEITRLLLEGISSTHAHHLRCLNDFVEAQMTYYAQ
CYQYMLDLQKQLGSFPSNYLSNNNQTSVTPVPSVLPNAIGSSAMASTSGLVITSPSNLSD
LKECSGSRKARVLYDYDAANSTELSLLADEVITVFSVVGMDSDWLMGERGNQKGKVPITY
LELLN

ORF+Vector

Nucleotide Sequence (with vector) for pFN21AA0491 Download

>pFN21AA0491  5800 bp
TCAATATTGGCCATTAGCCATATTATTCATTGGTTATATAGCATAAATCAATATTGGCTA
TTGGCCATTGCATACGTTGTATCTATATCATAATATGTACATTTATATTGGCTCATGTCC
AATATGACCGCCATGTTGGCATTGATTATTGACTAGTTATTAATAGTAATCAATTACGGG
GTCATTAGTTCATAGCCCATATATGGAGTTCCGCGTTACATAACTTACGGTAAATGGCCC
GCCTGGCTGACCGCCCAACGACCCCCGCCCATTGACGTCAATAATGACGTATGTTCCCAT
AGTAACGCCAATAGGGACTTTCCATTGACGTCAATGGGTGGAGTATTTACGGTAAACTGC
CCACTTGGCAGTACATCAAGTGTATCATATGCCAAGTCCGCCCCCTATTGACGTCAATGA
CGGTAAATGGCCCGCCTGGCATTATGCCCAGTACATGACCTTACGGGACTTTCCTACTTG
GCAGTACATCTACGTATTAGTCATCGCTATTACCATGGTGATGCGGTTTTGGCAGTACAC
CAATGGGCGTGGATAGCGGTTTGACTCACGGGGATTTCCAAGTCTCCACCCCATTGACGT
CAATGGGAGTTTGTTTTGGCACCAAAATCAACGGGACTTTCCAAAATGTCGTAATAACCC
CGCCCCGTTGACGCAAATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGC
TGGTTTAGTGAACCGTCAGATCACTAGAAGCTTTATTGCGGTAGTTTATCACAGTTAAAT
TGCTAACGCAGTCAGTGCTTCTGACACAACAGTCTCGAACTTAAGCTGCAGAAGTTGGTC
GTGAGGCACTGGGCAGGTAAGTATCAAGGTTACAAGACAGGTTTAAGGAGACCAATAGAA
ACTGGGCTTGTCGAGACAGAGAAGACTCTTGCGTTTCTGATAGGCACCTATTGGTCTTAC
TGACATCCACTTTGCCTTTCTCTCCACAGGTGTCCACTCCCAGTTCAATTACAGCTCTTA
AGGCTAGAGTATTAATACGACTCACTATAGGGCTAGCAAAGCCACCATGGCAGAAATCGG
TACTGGCTTTCCATTCGACCCCCATTATGTGGAAGTCCTGGGCGAGCGCATGCACTACGT
CGATGTTGGTCCGCGCGATGGCACCCCTGTGCTGTTCCTGCACGGTAACCCGACCTCCTC
CTACGTGTGGCGCAACATCATCCCGCATGTTGCACCGACCCATCGCTGCATTGCTCCAGA
CCTGATCGGTATGGGCAAATCCGACAAACCAGACCTGGGTTATTTCTTCGACGACCACGT
CCGCTTCATGGATGCCTTCATCGAAGCCCTGGGTCTGGAAGAGGTCGTCCTGGTCATTCA
CGACTGGGGCTCCGCTCTGGGTTTCCACTGGGCCAAGCGCAATCCAGAGCGCGTCAAAGG
TATTGCATTTATGGAGTTCATCCGCCCTATCCCGACCTGGGACGAATGGCCAGAATTTGC
CCGCGAGACCTTCCAGGCCTTCCGCACCACCGACGTCGGCCGCAAGCTGATCATCGATCA
GAACGTTTTTATCGAGGGTACGCTGCCGATGGGTGTCGTCCGCCCGCTGACTGAAGTCGA
GATGGACCATTACCGCGAGCCGTTCCTGAATCCTGTTGACCGCGAGCCACTGTGGCGCTT
CCCAAACGAGCTGCCAATCGCCGGTGAGCCAGCGAACATCGTCGCGCTGGTCGAAGAATA
CATGGACTGGCTGCACCAGTCCCCTGTCCCGAAGCTGCTGTTCTGGGGCACCCCAGGCGT
TCTGATCCCACCGGCCGAAGCCGCTCGCCTGGCCAAAAGCCTGCCTAACTGCAAGGCTGT
GGACATCGGCCCGGGTCTGAATCTGCTGCAAGAAGACAACCCGGACCTGATCGGCAGCGA
GATCGCGCGCTGGCTGTCGACGCTCGAGATTTCCGGCGAGCCAACCACTGAGGATCTGTA
CTTTCAGAGCGATAACGCGATCGCCATGAATATCATGGACTTCAACGTGAAGAAGCTGGC
GGCCGACGCAGGCACCTTCCTCAGTCGCGCCGTGCAGTTCACAGAAGAAAAGCTTGGCCA
GGCTGAGAAGACAGAATTGGATGCTCACTTAGAGAACCTCCTTAGCAAAGCTGAATGTAC
CAAAATATGGACAGAAAAAATAATGAAACAAACTGAAGTGTTATTGCAGCCAAATCCAAA
TGCCAGGATAGAAGAATTTGTTTATGAGAAACTGGATAGAAAAGCTCCAAGTCGTATAAA
CAACCCAGAACTTTTGGGACAATATATGATTGATGCAGGGACTGAGTTTGGCCCAGGAAC
AGCTTATGGTAATGCCCTTATTAAATGTGGAGAAACCCAAAAAAGAATTGGAACAGCAGA
CAGAGAACTGATTCAAACGTCAGCCTTAAATTTTCTTACTCCTTTAAGAAACTTTATAGA
AGGAGATTACAAAACAATTGCTAAAGAAAGGAAACTATTGCAAAATAAGAGACTGGATTT
GGATGCTGCAAAAACGAGACTAAAAAAGGCAAAAGCTGCAGAAACTAGAAATTCATCTGA
ACAGGAATTAAGAATAACTCAAAGTGAATTTGATCGTCAAGCAGAGATTACCAGACTTCT
GCTAGAGGGAATCAGCAGTACACATGCCCATCACCTTCGCTGTCTGAATGACTTTGTAGA
AGCCCAGATGACTTACTATGCACAGTGTTACCAGTATATGTTGGACCTCCAGAAACAACT
GGGAAGTTTTCCATCCAATTATCTTAGTAACAACAATCAGACTTCTGTGACACCTGTACC
ATCAGTTTTACCAAATGCGATTGGTTCTTCTGCCATGGCTTCAACAAGTGGCCTAGTAAT
CACCTCTCCTTCCAACCTCAGTGACCTTAAGGAGTGTAGTGGCAGCAGAAAGGCCAGGGT
TCTCTATGATTATGATGCAGCAAACAGTACTGAATTATCACTTCTGGCAGATGAGGTGAT
CACTGTGTTCAGTGTTGTTGGAATGGATTCAGACTGGCTAATGGGGGAAAGGGGAAACCA
GAAGGGCAAGGTGCCAATTACCTACTTAGAACTGCTCAATGTTTAAACGAATTCGGGCTC
GGTACCCGGGGATCCTCTAGAGTCGACCTGCAGGCATGCAAGCTGATCCGGCTGCTAACA
AAGCCCGAAAGGAAGCTGAGTTGGCTGCTGCCACCGCTGAGCAATAACTAGCATAACCCC
TTGGGGCGGCCGCTTCGAGCAGACATGATAAGATACATTGATGAGTTTGGACAAACCACA
ACTAGAATGCAGTGAAAAAAATGCTTTATTTGTGAAATTTGTGATGCTATTGCTTTATTT
GTAACCATTATAAGCTGCAATAAACAAGTTAACAACAACAATTGCATTCATTTTATGTTT
CAGGTTCAGGGGGAGATGTGGGAGGTTTTTTTAAGCAAGTAAAACCTCTACAAATGTGGT
AAAATCGAATTCTAATGGATCCTCTTTGCGCTTGCGTTTTCCCTTGTCCAGATAGCCCAG
TAGCTGACATTCATCCGGGGTCAGCACCGTTTCTGCGGACTGGCTTTCTACGTAATGGTT
TCTTAGACGTCAGGTGGCACTTTTCGGGGAAATGTGCGCGGAACCCCTATTTGTTTATTT
TTCTAAATACATTCAAATATGTATCCGCTCATGAGACAATAACCCTGATAAATGCTTCAA
TAATATTGAAAAAGGAAGAGTATGAGTATTCAACATTTCCGTGTCGCCCTTATTCCCTTT
TTTGCGGCATTTTGCCTTCCTGTTTTTGCTCACCCAGAAACGCTGGTGAAAGTAAAAGAT
GCTGAAGATCAGTTGGGTGCACGAGTGGGTTACATCGAACTGGATCTCAACAGCGGTAAG
ATCCTTGAGAGTTTTCGCCCCGAAGAACGTTTTCCAATGATGAGCACTTTCAAAGTTCTG
CTATGTGGCGCGGTATTATCCCGTATTGACGCCGGGCAAGAGCAACTCGGTCGCCGCATA
CACTATTCTCAGAATGACTTGGTTGAGTACTCACCAGTCACAGAAAAGCATCTTACGGAT
GGCATGACAGTAAGAGAATTATGCAGTGCTGCCATAACCATGAGTGATAACACTGCGGCC
AACTTACTTCTGACAACTATCGGAGGACCGAAGGAGCTAACCGCTTTTTTGCACAACATG
GGGGATCATGTAACTCGCCTTGATCGTTGGGAACCGGAGCTGAATGAAGCCATACCAAAC
GACGAGCGTGACACCACGATGCCTGTAGCAATGGCAACAACGTTGCGCAAACTATTAACT
GGCGAACTACTTACTCTAGCTTCCCGGCAACAATTAATAGACTGGATGGAGGCGGATAAA
GTTGCAGGACCACTTCTGCGCTCGGCCCTTCCGGCTGGCTGGTTTATTGCTGATAAATCT
GGAGCCGGTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCAGATGGTAAGCCC
TCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGA
CAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAATTCGAAATGACCGACCAA
GCGACGCCCAACCGGTATCAGCTCACTCAAAGGCGGTAATACGGTTATCCACAGAATCAG
GGGATAACGCAGGAAAGAACATGTGAGCAAAAGGCCAGCAAAAGGCCAGGAACCGTAAAA
AGGCCGCGTTGCTGGCGTTTTTCCATAGGCTCCGCCCCCCTGACGAGCATCACAAAAATC
GACGCTCAAGTCAGAGGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCC
CTGGAAGCTCCCTCGTGCGCTCTCCTGTTCCGACCCTGCCGCTTACCGGATACCTGTCCG
CCTTTCTCCCTTCGGGAAGCGTGGCGCTTTCTCATAGCTCACGCTGTAGGTATCTCAGTT
CGGTGTAGGTCGTTCGCTCCAAGCTGGGCTGTGTGCACGAACCCCCCGTTCAGCCCGACC
GCTGCGCCTTATCCGGTAACTATCGTCTTGAGTCCAACCCGGTAAGACACGACTTATCGC
CACTGGCAGCAGCCACTGGTAACAGGATTAGCAGAGCGAGGTATGTAGGCGGTGCTACAG
AGTTCTTGAAGTGGTGGCCTAACTACGGCTACACTAGAAGGACAGTATTTGGTATCTGCG
CTCTGCTGAAGCCAGTTACCTTCGGAAAAAGAGTTGGTAGCTCTTGATCCGGCAAACAAA
CCACCGCTGGTAGCGGTGGTTTTTTTGTTTGCAAGCAGCAGATTACGCGCAGAAAAAAAG
GATTTCAAGAAGATCCTTTGATCTTTTCTACGGGGTCTGACGCTCAGTGGAACGAAAACT
CACGTTAAGGGATTTTGGTCATGAGATTATCAAAAAGGATCTTCACCTAGATCCTTTTAT
AGTCCGGAAATACAGGAACGCACGCTGGATGGCCCTTCGCTGGGATGGTGAAACCATGAA
AAATGGCAGCTTCAGTGGATTAAGTGGGGGTAATGTGGCCTGTACCCTCTGGTTGCATAG
GTATTCATACGGTTAAAATTTATCAGGCGCGATTGCGGCAGTTTTTCGGGTGGTTTGTTG
CCATTTTTACCTGTCTGCTGCCGTGATCGCGCTGAACGCGTTTTAGCGGTGCGTACAATT
AAGGGATTATGGTAAATCCACTTACTGTCTGCCCTCGTAGCCATCGAGATAAACCGCAGT
ACTCCGGCCACGATGCGTCCGGCGTAGAGGATCGAGATCT