PPM1F ORF clone FHC00003

KOP
KOP HaloTag ORF List Flexi search

Halotag ORF Clone FHC00003

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Clone info.

Species

human

Clone Name

pFN21AA0015 Sequence (with vector) Restriction Map

Product ID

FHC00003

Flexi Clone PID

FXC00003

ORF Sequence

DNA sequence (1362 bp)
Protein sequence (454 aa)

Linker Type

Flexi type

more Linker info

Vector Type

pFN21A Vector sequence

Restriction Map

Cloning Site

SgfI-PmeI

GenBank Accession

ORF sequence is derived from [AB384430]

Gene

KIAA0015

Symbol

PPM1F

Alias : CAMKP, CaMKPase, FEM-2, POPX2, hFEM-2

Description

protein phosphatase, Mg2+/Mn2+ dependent, 1F

Original Clone ID

ha00478

Quality control data of this clone

Description

Characterization of the cloned ORF

5'-end(FN21F1)

3'-end(F1KR)

Agarose gel electrophoretic image

5'/3'-end sequences (chromatogram)

N-terminal HaloTag-fusions expressed in HEK293 visualized by HaloTag TMR ligand

Cell image

SDS-PAGE

Features of the cloned ORF sequence

Description

Length: 1362 bp
Restriction map A Restriction map B
Genomic Structure

Features of the protein sequence

Description

Length: 454 aa
Result of homology search against CCDS protein database ( FASTA output, Multiple alignment) Entry Exp ID% symbol CCDS13796.1 1.1e-160 100.0 PPM1F CCDS11613.1 4.4e-56 47.1 PPM1E CCDS3629.1 2.3e-14 36.4 PPM1K CCDS46115.1 8e-13 35.4 PPM1N CCDS33886.1 1.9e-11 31.3 PPM1L The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.
Result of motif / domain search (InterProScan and SOSUI) [ zoom out (x2) ]
Result of InterProScan Search method interpro_ID From To Entry Definition HMMPfam IPR001932 157 406 PF00481 Protein phosphatase 2C-like HMMSmart IPR001932 144 411 SM00332 Protein phosphatase 2C-like IPR001932 171 413 SM00331 Protein phosphatase 2C-like Inquiries or Suggestions ? Send a message to flexiclone AT kazusagt.com

ORF DNA

Cloned ORF Nucleotide sequence for pFN21AA0015 Download

>KIAA0015  1362 bp
ATGTCCTCTGGAGCCCCACAGAAGAGCAGCCCAATGGCCAGTGGAGCTGAGGAGACCCCA
GGCTTCCTGGACACGCTCCTGCAAGACTTCCCAGCCCTGCTGAACCCAGAGGACCCTCTG
CCATGGAAGGCCCCAGGGACGGTGCTCAGCCAGGAGGAGGTGGAGGGCGAGCTGGCTGAG
CTGGCCATGGGCTTTCTGGGCAGCAGGAAGGCCCCGCCACCACTTGCTGCTGCTCTGGCC
CACGAAGCAGTTTCACAGCTGCTACAGACAGACCTTTCCGAATTCAGGAAGTTGCCCAGG
GAGGAAGAAGAAGAGGAGGAGGACGATGACGAGGAGGAAAAGGCCCCTGTGACCTTGCTG
GATGCCCAAAGCCTGGCACAGAGTTTCTTTAACCGCCTTTGGGAAGTCGCCGGCCAGTGG
CAGAAGCAGGTGCCATTGGCTGCCCGGGCCTCACAGCGGCAGTGGCTGGTCTCCATCCAC
GCCATCCGGAACACTCGCCGCAAGATGGAGGACCGGCACGTGTCCCTCCCTTCCTTCAAC
CAGCTCTTCGGCTTGTCTGACCCTGTGAACCGCGCCTACTTTGCTGTGTTTGATGGTCAC
GGAGGCGTGGATGCTGCGAGGTACGCCGCTGTCCACGTGCACACCAACGCTGCCCGCCAG
CCAGAGCTGCCCACAGACCCTGAGGGAGCCCTCAGAGAAGCCTTCCGGCGCACCGACCAG
ATGTTTCTCAGGAAAGCCAAGCGAGAGCGGCTGCAGAGCGGCACCACAGGTGTGTGTGCG
CTCATTGCAGGAGCGACCCTGCACGTCGCCTGGCTCGGGGATTCCCAGGTCATTTTGGTA
CAGCAGGGACAGGTGGTGAAGCTGATGGAGCCACACAGACCAGAACGGCAGGATGAGAAG
GCGCGCATTGAAGCATTGGGTGGCTTTGTGTCTCACATGGACTGCTGGAGAGTCAACGGG
ACCCTGGCCGTCTCCAGAGCCATCGGGGATGTCTTCCAGAAGCCCTACGTGTCTGGGGAG
GCCGATGCAGCTTCCCGGGCGCTGACGGGCTCCGAGGACTACCTGCTGCTTGCCTGTGAT
GGCTTCTTTGACGTCGTACCCCACCAGGAAGTTGTTGGCCTGGTCCAGAGCCACCTGACC
AGGCAGCAGGGCAGCGGGCTCCGTGTCGCCGAGGAGCTGGTGGCTGCGGCCCGGGAGCGG
GGCTCCCACGACAACATCACGGTCATGGTGGTCTTCCTCAGGGACCCCCAAGAGCTGCTG
GAGGGCGGGAACCAGGGAGAAGGGGACCCCCAGGCAGAAGGGAGGAGGCAGGACTTGCCC
TCCAGCCTTCCAGAACCTGAGACCCAGGCTCCACCAAGAAGC

ORF Protein

Cloned ORF protein sequence for pFN21AA0015 Download

>KIAA0015  454 aa
MSSGAPQKSSPMASGAEETPGFLDTLLQDFPALLNPEDPLPWKAPGTVLSQEEVEGELAE
LAMGFLGSRKAPPPLAAALAHEAVSQLLQTDLSEFRKLPREEEEEEEDDDEEEKAPVTLL
DAQSLAQSFFNRLWEVAGQWQKQVPLAARASQRQWLVSIHAIRNTRRKMEDRHVSLPSFN
QLFGLSDPVNRAYFAVFDGHGGVDAARYAAVHVHTNAARQPELPTDPEGALREAFRRTDQ
MFLRKAKRERLQSGTTGVCALIAGATLHVAWLGDSQVILVQQGQVVKLMEPHRPERQDEK
ARIEALGGFVSHMDCWRVNGTLAVSRAIGDVFQKPYVSGEADAASRALTGSEDYLLLACD
GFFDVVPHQEVVGLVQSHLTRQQGSGLRVAEELVAAARERGSHDNITVMVVFLRDPQELL
EGGNQGEGDPQAEGRRQDLPSSLPEPETQAPPRS

ORF+Vector

Nucleotide Sequence (with vector) for pFN21AA0015 Download

>pFN21AA0015  6067 bp
TCAATATTGGCCATTAGCCATATTATTCATTGGTTATATAGCATAAATCAATATTGGCTA
TTGGCCATTGCATACGTTGTATCTATATCATAATATGTACATTTATATTGGCTCATGTCC
AATATGACCGCCATGTTGGCATTGATTATTGACTAGTTATTAATAGTAATCAATTACGGG
GTCATTAGTTCATAGCCCATATATGGAGTTCCGCGTTACATAACTTACGGTAAATGGCCC
GCCTGGCTGACCGCCCAACGACCCCCGCCCATTGACGTCAATAATGACGTATGTTCCCAT
AGTAACGCCAATAGGGACTTTCCATTGACGTCAATGGGTGGAGTATTTACGGTAAACTGC
CCACTTGGCAGTACATCAAGTGTATCATATGCCAAGTCCGCCCCCTATTGACGTCAATGA
CGGTAAATGGCCCGCCTGGCATTATGCCCAGTACATGACCTTACGGGACTTTCCTACTTG
GCAGTACATCTACGTATTAGTCATCGCTATTACCATGGTGATGCGGTTTTGGCAGTACAC
CAATGGGCGTGGATAGCGGTTTGACTCACGGGGATTTCCAAGTCTCCACCCCATTGACGT
CAATGGGAGTTTGTTTTGGCACCAAAATCAACGGGACTTTCCAAAATGTCGTAATAACCC
CGCCCCGTTGACGCAAATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGC
TGGTTTAGTGAACCGTCAGATCACTAGAAGCTTTATTGCGGTAGTTTATCACAGTTAAAT
TGCTAACGCAGTCAGTGCTTCTGACACAACAGTCTCGAACTTAAGCTGCAGAAGTTGGTC
GTGAGGCACTGGGCAGGTAAGTATCAAGGTTACAAGACAGGTTTAAGGAGACCAATAGAA
ACTGGGCTTGTCGAGACAGAGAAGACTCTTGCGTTTCTGATAGGCACCTATTGGTCTTAC
TGACATCCACTTTGCCTTTCTCTCCACAGGTGTCCACTCCCAGTTCAATTACAGCTCTTA
AGGCTAGAGTATTAATACGACTCACTATAGGGCTAGCAAAGCCACCATGGCAGAAATCGG
TACTGGCTTTCCATTCGACCCCCATTATGTGGAAGTCCTGGGCGAGCGCATGCACTACGT
CGATGTTGGTCCGCGCGATGGCACCCCTGTGCTGTTCCTGCACGGTAACCCGACCTCCTC
CTACGTGTGGCGCAACATCATCCCGCATGTTGCACCGACCCATCGCTGCATTGCTCCAGA
CCTGATCGGTATGGGCAAATCCGACAAACCAGACCTGGGTTATTTCTTCGACGACCACGT
CCGCTTCATGGATGCCTTCATCGAAGCCCTGGGTCTGGAAGAGGTCGTCCTGGTCATTCA
CGACTGGGGCTCCGCTCTGGGTTTCCACTGGGCCAAGCGCAATCCAGAGCGCGTCAAAGG
TATTGCATTTATGGAGTTCATCCGCCCTATCCCGACCTGGGACGAATGGCCAGAATTTGC
CCGCGAGACCTTCCAGGCCTTCCGCACCACCGACGTCGGCCGCAAGCTGATCATCGATCA
GAACGTTTTTATCGAGGGTACGCTGCCGATGGGTGTCGTCCGCCCGCTGACTGAAGTCGA
GATGGACCATTACCGCGAGCCGTTCCTGAATCCTGTTGACCGCGAGCCACTGTGGCGCTT
CCCAAACGAGCTGCCAATCGCCGGTGAGCCAGCGAACATCGTCGCGCTGGTCGAAGAATA
CATGGACTGGCTGCACCAGTCCCCTGTCCCGAAGCTGCTGTTCTGGGGCACCCCAGGCGT
TCTGATCCCACCGGCCGAAGCCGCTCGCCTGGCCAAAAGCCTGCCTAACTGCAAGGCTGT
GGACATCGGCCCGGGTCTGAATCTGCTGCAAGAAGACAACCCGGACCTGATCGGCAGCGA
GATCGCGCGCTGGCTGTCGACGCTCGAGATTTCCGGCGAGCCAACCACTGAGGATCTGTA
CTTTCAGAGCGATAACGCGATCGCCATGTCCTCTGGAGCCCCACAGAAGAGCAGCCCAAT
GGCCAGTGGAGCTGAGGAGACCCCAGGCTTCCTGGACACGCTCCTGCAAGACTTCCCAGC
CCTGCTGAACCCAGAGGACCCTCTGCCATGGAAGGCCCCAGGGACGGTGCTCAGCCAGGA
GGAGGTGGAGGGCGAGCTGGCTGAGCTGGCCATGGGCTTTCTGGGCAGCAGGAAGGCCCC
GCCACCACTTGCTGCTGCTCTGGCCCACGAAGCAGTTTCACAGCTGCTACAGACAGACCT
TTCCGAATTCAGGAAGTTGCCCAGGGAGGAAGAAGAAGAGGAGGAGGACGATGACGAGGA
GGAAAAGGCCCCTGTGACCTTGCTGGATGCCCAAAGCCTGGCACAGAGTTTCTTTAACCG
CCTTTGGGAAGTCGCCGGCCAGTGGCAGAAGCAGGTGCCATTGGCTGCCCGGGCCTCACA
GCGGCAGTGGCTGGTCTCCATCCACGCCATCCGGAACACTCGCCGCAAGATGGAGGACCG
GCACGTGTCCCTCCCTTCCTTCAACCAGCTCTTCGGCTTGTCTGACCCTGTGAACCGCGC
CTACTTTGCTGTGTTTGATGGTCACGGAGGCGTGGATGCTGCGAGGTACGCCGCTGTCCA
CGTGCACACCAACGCTGCCCGCCAGCCAGAGCTGCCCACAGACCCTGAGGGAGCCCTCAG
AGAAGCCTTCCGGCGCACCGACCAGATGTTTCTCAGGAAAGCCAAGCGAGAGCGGCTGCA
GAGCGGCACCACAGGTGTGTGTGCGCTCATTGCAGGAGCGACCCTGCACGTCGCCTGGCT
CGGGGATTCCCAGGTCATTTTGGTACAGCAGGGACAGGTGGTGAAGCTGATGGAGCCACA
CAGACCAGAACGGCAGGATGAGAAGGCGCGCATTGAAGCATTGGGTGGCTTTGTGTCTCA
CATGGACTGCTGGAGAGTCAACGGGACCCTGGCCGTCTCCAGAGCCATCGGGGATGTCTT
CCAGAAGCCCTACGTGTCTGGGGAGGCCGATGCAGCTTCCCGGGCGCTGACGGGCTCCGA
GGACTACCTGCTGCTTGCCTGTGATGGCTTCTTTGACGTCGTACCCCACCAGGAAGTTGT
TGGCCTGGTCCAGAGCCACCTGACCAGGCAGCAGGGCAGCGGGCTCCGTGTCGCCGAGGA
GCTGGTGGCTGCGGCCCGGGAGCGGGGCTCCCACGACAACATCACGGTCATGGTGGTCTT
CCTCAGGGACCCCCAAGAGCTGCTGGAGGGCGGGAACCAGGGAGAAGGGGACCCCCAGGC
AGAAGGGAGGAGGCAGGACTTGCCCTCCAGCCTTCCAGAACCTGAGACCCAGGCTCCACC
AAGAAGCGTTTAAACGAATTCGGGCTCGGTACCCGGGGATCCTCTAGAGTCGACCTGCAG
GCATGCAAGCTGATCCGGCTGCTAACAAAGCCCGAAAGGAAGCTGAGTTGGCTGCTGCCA
CCGCTGAGCAATAACTAGCATAACCCCTTGGGGCGGCCGCTTCGAGCAGACATGATAAGA
TACATTGATGAGTTTGGACAAACCACAACTAGAATGCAGTGAAAAAAATGCTTTATTTGT
GAAATTTGTGATGCTATTGCTTTATTTGTAACCATTATAAGCTGCAATAAACAAGTTAAC
AACAACAATTGCATTCATTTTATGTTTCAGGTTCAGGGGGAGATGTGGGAGGTTTTTTTA
AGCAAGTAAAACCTCTACAAATGTGGTAAAATCGAATTCTAATGGATCCTCTTTGCGCTT
GCGTTTTCCCTTGTCCAGATAGCCCAGTAGCTGACATTCATCCGGGGTCAGCACCGTTTC
TGCGGACTGGCTTTCTACGTAATGGTTTCTTAGACGTCAGGTGGCACTTTTCGGGGAAAT
GTGCGCGGAACCCCTATTTGTTTATTTTTCTAAATACATTCAAATATGTATCCGCTCATG
AGACAATAACCCTGATAAATGCTTCAATAATATTGAAAAAGGAAGAGTATGAGTATTCAA
CATTTCCGTGTCGCCCTTATTCCCTTTTTTGCGGCATTTTGCCTTCCTGTTTTTGCTCAC
CCAGAAACGCTGGTGAAAGTAAAAGATGCTGAAGATCAGTTGGGTGCACGAGTGGGTTAC
ATCGAACTGGATCTCAACAGCGGTAAGATCCTTGAGAGTTTTCGCCCCGAAGAACGTTTT
CCAATGATGAGCACTTTCAAAGTTCTGCTATGTGGCGCGGTATTATCCCGTATTGACGCC
GGGCAAGAGCAACTCGGTCGCCGCATACACTATTCTCAGAATGACTTGGTTGAGTACTCA
CCAGTCACAGAAAAGCATCTTACGGATGGCATGACAGTAAGAGAATTATGCAGTGCTGCC
ATAACCATGAGTGATAACACTGCGGCCAACTTACTTCTGACAACTATCGGAGGACCGAAG
GAGCTAACCGCTTTTTTGCACAACATGGGGGATCATGTAACTCGCCTTGATCGTTGGGAA
CCGGAGCTGAATGAAGCCATACCAAACGACGAGCGTGACACCACGATGCCTGTAGCAATG
GCAACAACGTTGCGCAAACTATTAACTGGCGAACTACTTACTCTAGCTTCCCGGCAACAA
TTAATAGACTGGATGGAGGCGGATAAAGTTGCAGGACCACTTCTGCGCTCGGCCCTTCCG
GCTGGCTGGTTTATTGCTGATAAATCTGGAGCCGGTGAGCGTGGGTCTCGCGGTATCATT
GCAGCACTGGGGCCAGATGGTAAGCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGT
CAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAG
CATTGGTAATTCGAAATGACCGACCAAGCGACGCCCAACCGGTATCAGCTCACTCAAAGG
CGGTAATACGGTTATCCACAGAATCAGGGGATAACGCAGGAAAGAACATGTGAGCAAAAG
GCCAGCAAAAGGCCAGGAACCGTAAAAAGGCCGCGTTGCTGGCGTTTTTCCATAGGCTCC
GCCCCCCTGACGAGCATCACAAAAATCGACGCTCAAGTCAGAGGTGGCGAAACCCGACAG
GACTATAAAGATACCAGGCGTTTCCCCCTGGAAGCTCCCTCGTGCGCTCTCCTGTTCCGA
CCCTGCCGCTTACCGGATACCTGTCCGCCTTTCTCCCTTCGGGAAGCGTGGCGCTTTCTC
ATAGCTCACGCTGTAGGTATCTCAGTTCGGTGTAGGTCGTTCGCTCCAAGCTGGGCTGTG
TGCACGAACCCCCCGTTCAGCCCGACCGCTGCGCCTTATCCGGTAACTATCGTCTTGAGT
CCAACCCGGTAAGACACGACTTATCGCCACTGGCAGCAGCCACTGGTAACAGGATTAGCA
GAGCGAGGTATGTAGGCGGTGCTACAGAGTTCTTGAAGTGGTGGCCTAACTACGGCTACA
CTAGAAGGACAGTATTTGGTATCTGCGCTCTGCTGAAGCCAGTTACCTTCGGAAAAAGAG
TTGGTAGCTCTTGATCCGGCAAACAAACCACCGCTGGTAGCGGTGGTTTTTTTGTTTGCA
AGCAGCAGATTACGCGCAGAAAAAAAGGATTTCAAGAAGATCCTTTGATCTTTTCTACGG
GGTCTGACGCTCAGTGGAACGAAAACTCACGTTAAGGGATTTTGGTCATGAGATTATCAA
AAAGGATCTTCACCTAGATCCTTTTATAGTCCGGAAATACAGGAACGCACGCTGGATGGC
CCTTCGCTGGGATGGTGAAACCATGAAAAATGGCAGCTTCAGTGGATTAAGTGGGGGTAA
TGTGGCCTGTACCCTCTGGTTGCATAGGTATTCATACGGTTAAAATTTATCAGGCGCGAT
TGCGGCAGTTTTTCGGGTGGTTTGTTGCCATTTTTACCTGTCTGCTGCCGTGATCGCGCT
GAACGCGTTTTAGCGGTGCGTACAATTAAGGGATTATGGTAAATCCACTTACTGTCTGCC
CTCGTAGCCATCGAGATAAACCGCAGTACTCCGGCCACGATGCGTCCGGCGTAGAGGATC
GAGATCT