HDHD2 ORF clone FXC05407

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Flexi ORF Clone FXC05407

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Clone info.

Species

human

Clone Name

pF1KE0110 Sequence (with vector) Restriction Map

Product ID

FXC05407

HaloTag Clone PID

FHC05407

ORF Sequence

DNA sequence (777 bp)
Protein sequence (259 aa)

Linker Type

Flexi type

more Linker info

Vector Type

pF1K Vector sequence

Restriction Map

Cloning Site

SgfI-PmeI
Because the PmeI site of this clone is affected by EcoKI methylase, we strongly recommend use of EcoKI methylase-free E.coli strain for preparation of this clone.

GenBank Accession

ORF sequence is derived from [AB528146]

Gene

KIEE0110

Symbol

HDHD2

Alias : 3110052N05Rik, HEL-S-301

Description

haloacid dehalogenase-like hydrolase domain containing 2

Original Clone ID

oc00810

Features of the cloned ORF sequence

Description

Length: 777 bp
Restriction map A Restriction map B
Genomic Structure

Features of the protein sequence

Description

Length: 259 aa
Result of homology search against CCDS protein database ( FASTA output, Multiple alignment) Entry Exp ID% symbol CCDS32829.1 1.9e-115 100.0 HDHD2 CCDS7640.1 2.9e-40 43.6 LHPP CCDS53587.1 1.7e-28 42.2 LHPP CCDS81519.1 3.3e-28 41.1 LHPP The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.
Result of motif / domain search (InterProScan and SOSUI) [ zoom out (x2) ]
Result of InterProScan Search method interpro_ID From To Entry Definition HMMPfam IPR005834 8 216 PF00702 Haloacid dehalogenase-like hydrolase HMMTigr IPR006355 7 257 TIGR01458 HAD-superfamily hydrolase IPR006357 10 226 TIGR01460 HAD-superfamily hydrolase Inquiries or Suggestions ? Send a message to flexiclone AT kazusagt.com

ORF DNA

Cloned ORF Nucleotide sequence for pF1KE0110 Download

>KIEE0110  777 bp
ATGGCAGCATGCCGTGCATTAAAAGCTGTTTTGGTAGATCTCAGTGGCACACTTCACATT
GAAGATGCAGCTGTGCCAGGCGCACAGGAAGCTCTTAAAAGGTTACGTGGTGCTTCTGTA
ATCATTAGGTTTGTGACCAATACAACCAAAGAGAGCAAGCAAGACCTGTTAGAAAGGTTG
AGAAAATTGGAATTTGATATCTCTGAAGATGAAATATTCACATCTCTGACTGCAGCCAGA
AGTTTACTAGAGCGGAAACAAGTCAGACCCATGCTGCTAGTTGATGATCGGGCACTACCT
GATTTCAAAGGAATACAAACAAGTGATCCTAATGCTGTGGTCATGGGATTGGCACCAGAA
CATTTTCATTATCAAATTCTGAATCAAGCATTCCGGTTACTCCTGGATGGAGCACCTCTG
ATAGCAATCCACAAAGCCAGGTATTACAAGAGGAAAGATGGCTTAGCCCTGGGGCCTGGA
CCATTTGTGACTGCTTTAGAGTATGCCACAGATACCAAAGCCACAGTCGTGGGGAAACCA
GAGAAGACGTTCTTTTTGGAAGCATTGCGGGGCACTGGCTGTGAACCTGAGGAGGCTGTC
ATGATAGGAGATGATTGCAGGGATGATGTTGGTGGGGCTCAAGATGTCGGCATGCTGGGC
ATCTTAGTAAAGACTGGGAAATATCGAGCATCAGATGAAGAAAAAATTAATCCACCTCCT
TACTTAACTTGTGAGAGTTTCCCTCATGCTGTGGACCACATTCTGCAGCACCTATTG

ORF Protein

Cloned ORF protein sequence for pF1KE0110 Download

>KIEE0110  259 aa
MAACRALKAVLVDLSGTLHIEDAAVPGAQEALKRLRGASVIIRFVTNTTKESKQDLLERL
RKLEFDISEDEIFTSLTAARSLLERKQVRPMLLVDDRALPDFKGIQTSDPNAVVMGLAPE
HFHYQILNQAFRLLLDGAPLIAIHKARYYKRKDGLALGPGPFVTALEYATDTKATVVGKP
EKTFFLEALRGTGCEPEEAVMIGDDCRDDVGGAQDVGMLGILVKTGKYRASDEEKINPPP
YLTCESFPHAVDHILQHLL

ORF+Vector

Nucleotide Sequence (with vector) for pF1KE0110 Download

>pF1KE0110  3890 bp
GGATCTCGATCCCGCGAAATTAATACGACTCACTATAGGGGAATTGTGAGCGGATAACAA
TTCCCCACTAGTAATAATTTTCTTTAACTTTAGTAAGGAGCGATCGCCATGGCAGCATGC
CGTGCATTAAAAGCTGTTTTGGTAGATCTCAGTGGCACACTTCACATTGAAGATGCAGCT
GTGCCAGGCGCACAGGAAGCTCTTAAAAGGTTACGTGGTGCTTCTGTAATCATTAGGTTT
GTGACCAATACAACCAAAGAGAGCAAGCAAGACCTGTTAGAAAGGTTGAGAAAATTGGAA
TTTGATATCTCTGAAGATGAAATATTCACATCTCTGACTGCAGCCAGAAGTTTACTAGAG
CGGAAACAAGTCAGACCCATGCTGCTAGTTGATGATCGGGCACTACCTGATTTCAAAGGA
ATACAAACAAGTGATCCTAATGCTGTGGTCATGGGATTGGCACCAGAACATTTTCATTAT
CAAATTCTGAATCAAGCATTCCGGTTACTCCTGGATGGAGCACCTCTGATAGCAATCCAC
AAAGCCAGGTATTACAAGAGGAAAGATGGCTTAGCCCTGGGGCCTGGACCATTTGTGACT
GCTTTAGAGTATGCCACAGATACCAAAGCCACAGTCGTGGGGAAACCAGAGAAGACGTTC
TTTTTGGAAGCATTGCGGGGCACTGGCTGTGAACCTGAGGAGGCTGTCATGATAGGAGAT
GATTGCAGGGATGATGTTGGTGGGGCTCAAGATGTCGGCATGCTGGGCATCTTAGTAAAG
ACTGGGAAATATCGAGCATCAGATGAAGAAAAAATTAATCCACCTCCTTACTTAACTTGT
GAGAGTTTCCCTCATGCTGTGGACCACATTCTGCAGCACCTATTGGTTTAAACGAATTCG
AGCTCGGTACCCGGGGATCCTCTAGAGTCGACCTGCAGGCATGCAAGCTGATCCGGCTGC
TAACAAAGCCCGAAAGGAAGCTGAGTTGGCTGCTGCCACCGCTGAGCAATAACTAGCATA
ACCCCTTGGGGCCTCTAAACGGGTCTTGAGGGGTTTTTTGCTGAAAGGAGGAACTATATC
CGGTTCGCTTGCTGTCCATAAAACCGCCCAGTCTAGCTATCGCCATGTAAGCCCACTGCA
AGCTACCTGCTTTCTCTTTGCGCTTGCGTTTTCCCTTGTCCAGATAGCCCAGTAGCTGAC
ATTCATCCGGGGTCAGCACCGTTTCTGCGGACTGGCTTTCTACGTGTTCCGCTTCCTTTA
GCAGCCCTTGCGCCCTGAGTGCTTGCGGCAGCGTGAGCTTCAAAAGAATTGCCAGCTGGG
GCGCCCTCTGGTAAGGTTGGGAAGCCCTGCAAAGTAAACTGGATGGCTTTCTTGCCGCCA
AGGATCTGATGGCGCAGGGGATCAAGATCTGATCAAGAGACAGGATGACGGTCGTTTCGC
ATGCTTGAACAAGATGGATTGCACGCAGGTTCTCCGGCCGCTTGGGTGGAGAGGCTATTC
GGCTATGACTGGGCACAACAGACAATCGGCTGCTCTGATGCCGCCGTGTTCCGGCTGTCA
GCGCAGGGGCGCCCGGTTCTTTTTGTCAAGACCGACCTGTCCGGTGCCCTGAATGAACTG
CAGGACGAGGCAGCGCGGCTATCGTGGCTGGCCACGACGGGCGTTCCTTGCGCAGCTGTG
CTCGACGTTGTCACTGAAGCGGGAAGGGACTGGCTGCTATTGGGCGAAGTGCCGGGGCAG
GATCTCCTGTCATCTCACCTTGCTCCTGCCGAGAAAGTATCCATCATGGCTGATGCAATG
CGGCGGCTGCATACGCTTGATCCGGCTACCTGCCCATTCGACCACCAAGCGAAACATCGC
ATCGAGCGAGCACGCACTCGGATGGAAGCCGGTCTTGTCGATCAGGATGATCTGGACGAA
GAGCATCAGGGGCTCGCGCCAGCCGAACTGTTCGCCAGGCTCAAGGCGCGTATGCCGGAT
GGTGAGGATCTCGTCGTGACTCATGGCGATGCCTGCTTGCCGAATATCATGGTGGAAAAT
GGCCGCTTTTCTGGATTCATCGACTGTGGCCGGCTGGGTGTGGCGGACCGCTATCAGGAC
ATAGCGTTGGCTACCCGTGATATTGCTGAAGAGCTTGGCGGCGAATGGGCTGACCGCTTC
CTCGTGCTTTACGGTATCGCCGCTCCCGATTCGCAGCGCATCGCCTTCTATCGCCTTCTT
GACGAGTTCTTCTGAGCGGGACTCTGGGGTTCGAAATGACCGACCAAGCGACGCCCAACC
GGTATCAGCTCACTCAAAGGCGGTAATACGGTTATCCACAGAATCAGGGGATAACGCAGG
AAAGAACATGTGAGCAAAAGGCCAGCAAAAGGCCAGGAACCGTAAAAAGGCCGCGTTGCT
GGCGTTTTTCCATAGGCTCCGCCCCCCTGACGAGCATCACAAAAATCGACGCTCAAGTCA
GAGGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCCTGGAAGCTCCCT
CGTGCGCTCTCCTGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGCCTTTCTCCCTTC
GGGAAGCGTGGCGCTTTCTCATAGCTCACGCTGTAGGTATCTCAGTTCGGTGTAGGTCGT
TCGCTCCAAGCTGGGCTGTGTGCACGAACCCCCCGTTCAGCCCGACCGCTGCGCCTTATC
CGGTAACTATCGTCTTGAGTCCAACCCGGTAAGACACGACTTATCGCCACTGGCAGCAGC
CACTGGTAACAGGATTAGCAGAGCGAGGTATGTAGGCGGTGCTACAGAGTTCTTGAAGTG
GTGGCCTAACTACGGCTACACTAGAAGGACAGTATTTGGTATCTGCGCTCTGCTGAAGCC
AGTTACCTTCGGAAAAAGAGTTGGTAGCTCTTGATCCGGCAAACAAACCACCGCTGGTAG
CGGTGGTTTTTTTGTTTGCAAGCAGCAGATTACGCGCAGAAAAAAAGGATTTCAAGAAGA
TCCTTTGATCTTTTCTACGGGGTCTGACGCTCAGTGGAACGAAAACTCACGTTAAGGGAT
TTTGGTCATGAGATTATCAAAAAGGATCTTCACCTAGATCCTTTTATAGTCCGGAAATAC
AGGAACGCACGCTGGATGGCCCTTCGCTGGGATGGTGAAACCATGAAAAATGGCAGCTTC
AGTGGATTAAGTGGGGGTAATGTGGCCTGTACCCTCTGGTTGCATAGGTATTCATACGGT
TAAAATTTATCAGGCGCGATTGCGGCAGTTTTTCGGGTGGTTTGTTGCCATTTTTACCTG
TCTGCTGCCGTGATCGCGCTGAACGCGTTTTAGCGGTGCGTACAATTAAGGGATTATGGT
AAATCCACTTACTGTCTGCCCTCGTAGCCATCGAGATAAACCGCAGTACTCCGGCCACGA
TGCGTCCGGCGTAGAGGATCGAGATCTTTTCAGCCTGATACAGATTAAATCAGAACGCAG
AAGCGGTCTGATAAAACAGAATTTGCCTGGCGGCAGTAGCGCGGTGGTCCCACCTGACCC
CATGCCGAACTCAGAAGTGAAACGCCGTAGCGCCGATGGTAGTGTGGGGTCTCCCCATGC
GAGAGTAGGGAACTGCCAGGCATCAAATAAAACGAAAGGCTCAGTCGAAAGACTGGGCCT
TTCGTTTTATCTGTTGTTTGTCGGTGAACGCTCTCCTGAGTAGGACAAATCCGCCGGGAG
CGGATTTGAACGTTGCGAAGCAACGGCCCGGAGGGTGGCGGGCAGGACGCCCGCCATAAA
CTGCCAGGCATCAAATTAAGCAGAAGGCCATCCTGACGGATGGCCTTTTTGCGTTTCTAC
AAACTCTTTTGTTTATTTTTCTAAATACATTCAAATATGTATCCGCTCAT