RHOJ ORF clone FXC02121

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Flexi ORF Clone FXC02121

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Clone info.

Species

human

Clone Name

pF1KB4000 Sequence (with vector) Restriction Map

Product ID

FXC02121

HaloTag Clone PID

FHC02121

ORF Sequence

DNA sequence (642 bp)
Protein sequence (214 aa)

Linker Type

Flexi type

more Linker info

Vector Type

pF1K Vector sequence

Restriction Map

Cloning Site

SgfI-PmeI

GenBank Accession

ORF sequence is derived from [AB464131]

Gene

KIBB4000

Symbol

RHOJ

Alias : ARHJ, RASL7B, TC10B, TCL

Description

ras homolog family member J

Original Clone ID

fj12090

Features of the cloned ORF sequence

Description

Length: 642 bp
Restriction map A Restriction map B
Genomic Structure

Features of the protein sequence

Description

Length: 214 aa
Result of homology search against CCDS protein database ( FASTA output, Multiple alignment) Entry Exp ID% symbol CCDS9757.1 3.4e-90 99.5 RHOJ CCDS33191.1 6.1e-61 79.1 RHOQ CCDS221.1 1.2e-48 64.2 CDC42 CCDS222.1 7.4e-48 62.6 CDC42 CCDS5348.1 1.5e-46 61.2 RAC1 The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.
Result of motif / domain search (InterProScan and SOSUI) [ zoom out (x2) ]
Result of InterProScan Search method interpro_ID From To Entry Definition FPrintScan IPR001806 22 43 PR00449 Ras GTPase IPR001806 45 61 PR00449 Ras GTPase IPR001806 62 84 PR00449 Ras GTPase IPR001806 124 137 PR00449 Ras GTPase IPR001806 172 194 PR00449 Ras GTPase HMMPfam IPR013753 23 194 PF00071 Ras HMMSmart IPR003577 19 197 SM00173 Ras small GTPase IPR003579 22 197 SM00175 Ras small GTPase IPR003578 24 197 SM00174 Small GTPase HMMTigr IPR005225 21 172 TIGR00231 Small GTP-binding protein domain ProfileScan IPR003578 15 194 PS51420 Small GTPase
Result of homology search against disease genes in OMIM database(FASTA output, Multiple alignment) H_id H_len (aa) OMIM_No. ID (%) Q_ali/Q_len (%) NP_065714 214 607653 99.5 100.0 NP_036381 205 605857 79.1 90.2 NP_001782 191 116952,616737 64.2 86.4 NP_001034891 191 116952,616737 64.2 86.4 NP_426359 191 116952,616737 62.6 86.4 Inquiries or Suggestions ? Send a message to flexiclone AT kazusagt.com

ORF DNA

Cloned ORF Nucleotide sequence for pF1KB4000 Download

>KIBB4000  642 bp
ATGAACTGCAAAGAGGGAACTGACAGCAGCTGCGGCTGCAGGGGCAACGACGAGAAGAAG
ATGTTGAAGTGTGTGGTGGTGGGGGACGGTGCCGTGGGGAAAACCTGCCTGCTGATGAGC
TACGCCAACGACGCCTTCCCAGGGGAATACGTGCCCACTGTGTTTGACCACTATGCAGTT
ACTGTGACTGTGGGAGGCAAGCAACACTTGCTCGGACTGTATGACACCGCGGGACAGGAG
GACTACAACCAGCTGAGGCCACTCTCCTACCCCAACACGGATGTGTTTTTGATCTGCTTC
TCTGTCGTAAACCCTGCCTCTTACCACAATGTCCAGGAGGAATGGGTCCCCGAGCTCAAG
GACTGCATGCCTCACGTGCCTTATGTCCTCATAGGGACCCAGATTGATCTCCGTGATGAC
CCAAAAACCTTGGCCCGTTTGCTGTATATGAAAGAGAAACCTCTCACTTACGAGCATGGT
GTGAAGCTCGCAAAAGCGATCGGAGCACAGTGCTACTTGGAATGTTCAGCTCTGACTCAG
AAAGGTCTCAAAGCGGTTTTTGATGAAGCAATCCTCACCATTTTCCACCCCAAGAAAAAG
AAGAAACGCTGTTCTGAGGGTCACAGCTGCTGTTCAATTATC

ORF Protein

Cloned ORF protein sequence for pF1KB4000 Download

>KIBB4000  214 aa
MNCKEGTDSSCGCRGNDEKKMLKCVVVGDGAVGKTCLLMSYANDAFPGEYVPTVFDHYAV
TVTVGGKQHLLGLYDTAGQEDYNQLRPLSYPNTDVFLICFSVVNPASYHNVQEEWVPELK
DCMPHVPYVLIGTQIDLRDDPKTLARLLYMKEKPLTYEHGVKLAKAIGAQCYLECSALTQ
KGLKAVFDEAILTIFHPKKKKKRCSEGHSCCSII

ORF+Vector

Nucleotide Sequence (with vector) for pF1KB4000 Download

>pF1KB4000  3755 bp
GGATCTCGATCCCGCGAAATTAATACGACTCACTATAGGGGAATTGTGAGCGGATAACAA
TTCCCCACTAGTAATAATTTTCTTTAACTTTAGTAAGGAGCGATCGCCATGAACTGCAAA
GAGGGAACTGACAGCAGCTGCGGCTGCAGGGGCAACGACGAGAAGAAGATGTTGAAGTGT
GTGGTGGTGGGGGACGGTGCCGTGGGGAAAACCTGCCTGCTGATGAGCTACGCCAACGAC
GCCTTCCCAGGGGAATACGTGCCCACTGTGTTTGACCACTATGCAGTTACTGTGACTGTG
GGAGGCAAGCAACACTTGCTCGGACTGTATGACACCGCGGGACAGGAGGACTACAACCAG
CTGAGGCCACTCTCCTACCCCAACACGGATGTGTTTTTGATCTGCTTCTCTGTCGTAAAC
CCTGCCTCTTACCACAATGTCCAGGAGGAATGGGTCCCCGAGCTCAAGGACTGCATGCCT
CACGTGCCTTATGTCCTCATAGGGACCCAGATTGATCTCCGTGATGACCCAAAAACCTTG
GCCCGTTTGCTGTATATGAAAGAGAAACCTCTCACTTACGAGCATGGTGTGAAGCTCGCA
AAAGCGATCGGAGCACAGTGCTACTTGGAATGTTCAGCTCTGACTCAGAAAGGTCTCAAA
GCGGTTTTTGATGAAGCAATCCTCACCATTTTCCACCCCAAGAAAAAGAAGAAACGCTGT
TCTGAGGGTCACAGCTGCTGTTCAATTATCGTTTAAACGAATTCGAGCTCGGTACCCGGG
GATCCTCTAGAGTCGACCTGCAGGCATGCAAGCTGATCCGGCTGCTAACAAAGCCCGAAA
GGAAGCTGAGTTGGCTGCTGCCACCGCTGAGCAATAACTAGCATAACCCCTTGGGGCCTC
TAAACGGGTCTTGAGGGGTTTTTTGCTGAAAGGAGGAACTATATCCGGTTCGCTTGCTGT
CCATAAAACCGCCCAGTCTAGCTATCGCCATGTAAGCCCACTGCAAGCTACCTGCTTTCT
CTTTGCGCTTGCGTTTTCCCTTGTCCAGATAGCCCAGTAGCTGACATTCATCCGGGGTCA
GCACCGTTTCTGCGGACTGGCTTTCTACGTGTTCCGCTTCCTTTAGCAGCCCTTGCGCCC
TGAGTGCTTGCGGCAGCGTGAGCTTCAAAAGAATTGCCAGCTGGGGCGCCCTCTGGTAAG
GTTGGGAAGCCCTGCAAAGTAAACTGGATGGCTTTCTTGCCGCCAAGGATCTGATGGCGC
AGGGGATCAAGATCTGATCAAGAGACAGGATGACGGTCGTTTCGCATGCTTGAACAAGAT
GGATTGCACGCAGGTTCTCCGGCCGCTTGGGTGGAGAGGCTATTCGGCTATGACTGGGCA
CAACAGACAATCGGCTGCTCTGATGCCGCCGTGTTCCGGCTGTCAGCGCAGGGGCGCCCG
GTTCTTTTTGTCAAGACCGACCTGTCCGGTGCCCTGAATGAACTGCAGGACGAGGCAGCG
CGGCTATCGTGGCTGGCCACGACGGGCGTTCCTTGCGCAGCTGTGCTCGACGTTGTCACT
GAAGCGGGAAGGGACTGGCTGCTATTGGGCGAAGTGCCGGGGCAGGATCTCCTGTCATCT
CACCTTGCTCCTGCCGAGAAAGTATCCATCATGGCTGATGCAATGCGGCGGCTGCATACG
CTTGATCCGGCTACCTGCCCATTCGACCACCAAGCGAAACATCGCATCGAGCGAGCACGC
ACTCGGATGGAAGCCGGTCTTGTCGATCAGGATGATCTGGACGAAGAGCATCAGGGGCTC
GCGCCAGCCGAACTGTTCGCCAGGCTCAAGGCGCGTATGCCGGATGGTGAGGATCTCGTC
GTGACTCATGGCGATGCCTGCTTGCCGAATATCATGGTGGAAAATGGCCGCTTTTCTGGA
TTCATCGACTGTGGCCGGCTGGGTGTGGCGGACCGCTATCAGGACATAGCGTTGGCTACC
CGTGATATTGCTGAAGAGCTTGGCGGCGAATGGGCTGACCGCTTCCTCGTGCTTTACGGT
ATCGCCGCTCCCGATTCGCAGCGCATCGCCTTCTATCGCCTTCTTGACGAGTTCTTCTGA
GCGGGACTCTGGGGTTCGAAATGACCGACCAAGCGACGCCCAACCGGTATCAGCTCACTC
AAAGGCGGTAATACGGTTATCCACAGAATCAGGGGATAACGCAGGAAAGAACATGTGAGC
AAAAGGCCAGCAAAAGGCCAGGAACCGTAAAAAGGCCGCGTTGCTGGCGTTTTTCCATAG
GCTCCGCCCCCCTGACGAGCATCACAAAAATCGACGCTCAAGTCAGAGGTGGCGAAACCC
GACAGGACTATAAAGATACCAGGCGTTTCCCCCTGGAAGCTCCCTCGTGCGCTCTCCTGT
TCCGACCCTGCCGCTTACCGGATACCTGTCCGCCTTTCTCCCTTCGGGAAGCGTGGCGCT
TTCTCATAGCTCACGCTGTAGGTATCTCAGTTCGGTGTAGGTCGTTCGCTCCAAGCTGGG
CTGTGTGCACGAACCCCCCGTTCAGCCCGACCGCTGCGCCTTATCCGGTAACTATCGTCT
TGAGTCCAACCCGGTAAGACACGACTTATCGCCACTGGCAGCAGCCACTGGTAACAGGAT
TAGCAGAGCGAGGTATGTAGGCGGTGCTACAGAGTTCTTGAAGTGGTGGCCTAACTACGG
CTACACTAGAAGGACAGTATTTGGTATCTGCGCTCTGCTGAAGCCAGTTACCTTCGGAAA
AAGAGTTGGTAGCTCTTGATCCGGCAAACAAACCACCGCTGGTAGCGGTGGTTTTTTTGT
TTGCAAGCAGCAGATTACGCGCAGAAAAAAAGGATTTCAAGAAGATCCTTTGATCTTTTC
TACGGGGTCTGACGCTCAGTGGAACGAAAACTCACGTTAAGGGATTTTGGTCATGAGATT
ATCAAAAAGGATCTTCACCTAGATCCTTTTATAGTCCGGAAATACAGGAACGCACGCTGG
ATGGCCCTTCGCTGGGATGGTGAAACCATGAAAAATGGCAGCTTCAGTGGATTAAGTGGG
GGTAATGTGGCCTGTACCCTCTGGTTGCATAGGTATTCATACGGTTAAAATTTATCAGGC
GCGATTGCGGCAGTTTTTCGGGTGGTTTGTTGCCATTTTTACCTGTCTGCTGCCGTGATC
GCGCTGAACGCGTTTTAGCGGTGCGTACAATTAAGGGATTATGGTAAATCCACTTACTGT
CTGCCCTCGTAGCCATCGAGATAAACCGCAGTACTCCGGCCACGATGCGTCCGGCGTAGA
GGATCGAGATCTTTTCAGCCTGATACAGATTAAATCAGAACGCAGAAGCGGTCTGATAAA
ACAGAATTTGCCTGGCGGCAGTAGCGCGGTGGTCCCACCTGACCCCATGCCGAACTCAGA
AGTGAAACGCCGTAGCGCCGATGGTAGTGTGGGGTCTCCCCATGCGAGAGTAGGGAACTG
CCAGGCATCAAATAAAACGAAAGGCTCAGTCGAAAGACTGGGCCTTTCGTTTTATCTGTT
GTTTGTCGGTGAACGCTCTCCTGAGTAGGACAAATCCGCCGGGAGCGGATTTGAACGTTG
CGAAGCAACGGCCCGGAGGGTGGCGGGCAGGACGCCCGCCATAAACTGCCAGGCATCAAA
TTAAGCAGAAGGCCATCCTGACGGATGGCCTTTTTGCGTTTCTACAAACTCTTTTGTTTA
TTTTTCTAAATACATTCAAATATGTATCCGCTCAT