SSEARCH searches a sequence database
 using the Smith-Waterman algorithm
 version 2.0u54, July. 1996
Please cite:
 T. F. Smith and M. S. Waterman, (1981) J. Mol. Biol. 147:195-197; 
 W.R. Pearson (1991) Genomics 11:635-650

 >MPL12.13 (Universal genetic code) : 1022 aa
 vs  library
 searching celf25b4 library

    708 residues in     1 sequences
The best scores are:                              s-w
CELF25B4, 708 bases, 2250 checksum.                (708) 638

>>CELF25B4, 708 bases, 2250 checksum.                (708 aa)
Smith-Waterman score: 638;   25.623% identity in 722 aa overlap

                                                           10      
MPL12.                                             MVTTEVRTAVSDKEPL
                                                                   
CELF25 MHRTFERNMFHLEDHDVKRKREDSHRRRDDRHRDSDRDRDRRRRSRSRSNSNRHRTTGGS
               10        20        30        40        50        60

         20        30         40               50        60        
MPL12. QRSPELDSSTDFLDNDRL-KETFSS--GA--LD---FDEWTLLISEIETTSFPDDI----
       :::     ..:. :.. . . . .:  :.  .:   ::.:. ...... .   ::.    
CELF25 QRSRSSRHGSDYKDTSLMTRPSVASHFGTPPIDVADFDNWVNILAKVDQS---DDVDFAR
               70        80        90       100       110          

           70        80         90       100       110       120   
MPL12. EKLCLVYDAFLLEFPLCHGYWRKYA-YHKIKLCTLEDAVEVFERAVQAATYSVAVWLDYC
       ::    : .:: ..: :.:.:.::: :.: :. .. .:  :.:... .   :. .:: : 
CELF25 EK----YRSFLSRYPNCYGFWQKYAEYEK-KMGNIAEAKAVWEKGIISIPLSIDLWLGYT
           120       130       140        150       160       170  

           130         140       150       160                170  
MPL12. AFAVAAYED-PHDVSR-LFERGLSFIGKDYSCCTLWDKYI--E---YL--L--GQQQWSS
       :  :   .. : .  : :. :.. . : .:.   :: . :  :   :.  :  :. . : 
CELF25 A-DVKNIKNFPPESLRDLYARAIEIAGLEYQSDRLWLEAIGFERAVYMDELCKGNTNASC
             180       190       200       210       220       230 

              180           190       200                210       
MPL12. --LANVYLRTLKYPS----KKLDLYYKNFRKIAASL----KE-----KIKCRIDVNGDLS
         .. .. . :. :.    ...: : . .  :   :    .:     :. :. .. :  :
CELF25 KRIGVLFDKLLSTPTFHAPSHFDRYVQYLNTIEPHLLLSDREYEEIMKMVCK-QL-GK-S
             240       250       260       270       280           

       220         230         240         250         260         
MPL12. SDPM-EE-DLVHTRHTDEE--ISIVVRELMG--PSSSSAVSKALH--TYLSI--GEQFYQ
        . . .. .: .  .. :.  ..:...   :  : .   :..  :  : :..  ::   .
CELF25 IEELVQQVQLSYICQSGENGMLNIMTESAEGTFPIT---VNSLQHDPTALQLIRGEIVAR
      290       300       310       320          330       340     

       270              280       290       300       310          
MPL12. DSRQLMEK-IS-C-----FETQIRRPYFHVKPLDTNQLDNWHAYLSFGETY-GDFDWAIN
        .: ...: .. :     ::..:.::::::::::  :: :: .::.: :   :  . .  
CELF25 RKR-IYDKNMKECEIRAGFEANIKRPYFHVKPLDYPQLFNWMSYLDF-EIKEGHEERVKI
          350       360       370       380       390        400   

     320       330       340            350        360       370   
MPL12. LYERCLIPCANYTEFWFRYVDFV----ESKG-GRELANFALARAS-QTFVKSASVIHLFN
       :..::::::. : :::..:. ..    .::  .::.  .  :.    :   : ..  : .
CELF25 LFDRCLIPCSLYEEFWIKYARWTWKTYKSKTKSREI--YMKAKIHCPT---SLNLA-LSE
           410       420       430         440          450        

           380         390        400           410       420      
MPL12. ARFKEHVG--DASAASVALSRCGEEL-GFGFVE----NVTKKANMEKRLGNFEAAVTTYR
       . :.: :   : .: .. :.   ::  :. ..:    .: .. . ::. :.  .: . . 
CELF25 SGFEESVENFD-DAIKI-LDNFREEYPGYVLLELRYLGVLRRKS-EKE-GH--GAPSEF-
       460        470        480       490        500          510 

        430         440       450         460        470           
MPL12. EALNK--TLIGKENLETTARLYVQFS-RL-KYVITNSADDA-AQILLEGNENV-PHCKL-
        ..:.  .:: ... ...  :.  .: .: .:   .  :   :: .:.   .. :  .: 
CELF25 -VMNQYESLI-RDS-QSSPNLHSFYSLKLARYHQKSRRDPKLAQKVLKKAISIDPF-NLQ
                520        530       540       550       560       

     480       490       500       510       520       530         
MPL12. LLEELMRLLMMHGGSRQVDLLDPIIDKELSHQADSSDGLSAEDKEEISNLYMEFIDLSGT
       :  . . . .   .:.... :: .: . ..   ::.  :  ::: ..:.  ..:..  : 
CELF25 LYSQYVDIAY---SSESMSELD-VI-QSFDVALDSN--LRLEDKVRFSQRKLDFLEELG-
        570          580         590         600       610         

     540       550       560       570       580       590         
MPL12. IHDVRKALGRHIKLFPHSARAKLRGSRPSGNLFRELIQRREKTRERLNQDLLTNKGISSI
        ...  :.  : . : :    .: :. :. :.    :  :  ::  .: :   .. .. .
CELF25 -NNIL-AVEDH-RDF-H---YNLLGQLPD-NV---TI--R--TRF-VN-D--PSR-VA-L
       620         630           640                650            

     600        610       620       630       640       650        
MPL12. VDS-PPKEKKESSLDSYGTQSKDAVRADYVNTEPNQGCLTSGHLVEGNDNVIERETLCES
       . . ::...    . . . :.. :.  ...   : :  .   ...   .....  :: ::
CELF25 APAMPPQQQM---VYAPAIQQQPAM--NMMPMMPMQ--MP--YMA---SSAVQL-TLIES
       660          670         680         690             700    

      660       670       680       690       700       710        
MPL12. QSDLSMGLKANEGGKRSHEVSLPIQASPEHGFVTKQAHFSSNSVDTVKSDAIVIQPSGSQ
        :                                                          
CELF25 PSTN                                                        
                                                                   

      720       730       740       750       760       770        
MPL12. SPQSYQSQESLRQTGRNRYHRRDLNQMHRDSKPRSQERPPQMPYSPVGTGREILGQHMAF

      780       790       800       810       820       830        
MPL12. THQDNRVALQSSTSQNPQNQFQNSALQMHPVVQTSNAYPQSQIHGQHMIVSPPESQNPQN

      840       850       860       870       880       890        
MPL12. QCQNSTSQVQTSFAYPQTQIPQNPVQSNYQQEGQMQSHEAYNQMWQQYYYSYYYYQQQQQ

      900       910       920       930       940       950        
MPL12. LMSEQPQPNQNPQPQLDQNLVQLLSKQYQSQAKTQYLQPQQVEQVNTQQQSQEPQNQQQI

      960       970       980       990      1000      1010        
MPL12. QFQQQQQQQEWFQQQQQWQQQQYLLYIQQQQLQGEAKGDEQRLSMPQGSTTNSDIQKVCL

     1020  
MPL12. KIQT

Library scan:  0:00:00  total CPU time:  0:00:03