Data format You should prepare set.data, orf.data and clone.data(optional) before you run Make_Files.csh. If you have Sybase SQL Server System 10 or 11, follow 1( 1-1, 1-2, 1-3 ) if not, follow 1( 1-1 ), 2, 3 1. SET data format ( set.data ) set vars 1-1. Set vars for CircleMap and OrfMaP CircleMap circular map e.g. http://www.kazusa.or.jp/cyanobase/map/java/cmap_small/cmap_sm.html OrfMap e.g. http://www.kazusa.or.jp/cyanobase/map/java/orf_small/orf_sm1.html totalsize -> bp scalebase -> length in Kbp / line (only 10 kbp or 15kbp can be permitted) cloneflg -> use (on ) don't use(off) If you want to show cosmid/phage/plasmid clones in OrfMap, set clone flg on, if not off. cgidir -> path for cgi-bin files httpdir -> path for html document files # the Sybase value that stands for the direction of ORF direct_f -> forward direct_r -> reverse e.g. ---------------------------------------------- totalsize 3573470 scalebase 15 cloneflg on cgidir /usr/local/etc/cern-httpd/cgi-bin htmldir /usr/local/etc/cern-httpd/htdocs direct_f direct direct_r complement ---------------------------------------------- 1-2. SYBASE environments SYBASE -> $SYBASEpath ($SYBASE) DSQUERY -> dsquery ($DSQUERY) usr -> user name (sybase login name) passwd password (sybase password) dbase -> database name (sybase dbname) e.g. ------------------------------ SYBASE /usr/sybase/tool DSQUERY SYBASE usr cyano passwd abcdef dbase cyanodb ------------------------------ 1-3. Set the Sybase table and column name If you want to show cosmid/phage/plasmid clones in OrfMap, follow A, B if not, you need to follow A only. All data types are described in SybaseTable.doc. (A) ORF Tables and Columns (a) Table ORF_ID_Table -> ORF ID and name ORF_Data_Table -> ORF data table ORF_Color_Table -> ORF color table (b) Column ORF_ID -> ORF ID ORF_Name -> ORF name ORF_Begin -> ORF begin ORF_End -> ORF end ORF_length -> ORF length ORF_direction -> ORF direction ORF_classify -> classification of ORF ORF_color -> ORF color e.g. ---------------------------------- ORF_ID_Table id_vs_name_orf ORF_Data_Table orfdata ORF_Color_Table color ORF_ID orf_id ORF_Name orf_name ORF_Begin begin ORF_End end ORF_length aa_length ORF_direction direction ORF_classify classify ORF_color color_rgb ---------------------------------- (B) Clone Tables and Columns (a) Table CLONE_ID_Table -> CLONE ID and name CLONE_Data_Table -> CLONE data table (b) Column CLONE_ID -> CLONE ID CLONE_Name -> CLONE name CLONE_Pos_Begin -> CLONE begin CLONE_Pos_End -> CLONE end CLONE_Seq_Begin -> Sequenced region begin CLONE_Seq_End -> Sequenced region end CLONE_length -> Sequenced length e.g. ---------------------------------- CLONE_ID_Table id_vs_name_cl CLONE_Data_Table clonedata CLONE_ID clone_id CLONE_Name clone_name CLONE_Pos_Begin pos_begin CLONE_Pos_End pos_end CLONE_Seq_Begin seq_begin CLONE_Seq_End seq_end CLONE_length length ---------------------------------- 2. ORF data format ( orf.data ) format: ORF_Name ORF_Begin ORF_End ORF_length ORF_direction ORF_color e.g. ----------------------------------------------------- slr0612 937 185 direct 119,136,153 slr0613 1577 173 direct 255,255,255 sll0558 2172 233 complement 119,136,153 sll1214 3192 358 complement 0,0,0 sll1213 4451 312 complement 119,136,153 ----------------------------------------------------- 3. CLONE data format ( clone.data ) format: CLONE_Name CLONE_Pos_Begin CLONE_Pos_End CLONE_Seq_Begin CLONE_Seq_End CLONE_length e.g. ----------------------------------------------------------------------- cs0079 20918 59110 25608 59876 34269 cs0483 59877 98941 59877 97839 37963 cs0329 97840 140346 97840 135132 37293 -----------------------------------------------------------------------