Jatropha Genome Database

JcCB0148401.10
Show Alignment:  
BLASTP 2.2.23 [Feb-03-2010]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Reference for compositional score matrix adjustment: Altschul, Stephen F., 
John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis,
Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches
using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.

Query= JcCB0148401.10 - phase: 0 /pseudo
         (449 letters)

Database: castor_wgs_0.1_aa 
           31,221 sequences; 10,419,776 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

30170.m014359 starch branching enzyme II, putative                    731   0.0  
27496.m000095 starch branching enzyme II, putative                    448   e-126
29762.m000486 1,4-alpha-glucan branching enzyme, putative             241   7e-64
28323.m000041 alpha-amylase, putative                                  55   6e-08
29776.m000488 isoamylase, putative                                     52   7e-07
29908.m006089 isoamylase, putative                                     50   2e-06

>30170.m014359 starch branching enzyme II, putative
          Length = 914

 Score =  731 bits (1887), Expect = 0.0,   Method: Compositional matrix adjust.
 Identities = 368/454 (81%), Positives = 402/454 (88%), Gaps = 10/454 (2%)

Query: 1   MLASLDLFPAPAFGSVSPSLGKNFKRALGNNLH--KQKLIGLTFESRKLHGSPKFYFMPG 58
           ML SL LFPAP FGS    L  N KRA GN+ H  +QK +GL   S+ L G PK YF+  
Sbjct: 49  MLGSLTLFPAPVFGS----LANNSKRAFGNDCHITRQKPLGLIHRSQYLLGCPKVYFLSR 104

Query: 59  ISTNK--RHGLTITAVMTDDNTTMTN-EKEMEDIHLLSIDPGLGPFKDHFSYRMKRYRNQ 115
              NK  +H LT +AVMTDD++T TN +++ME+I LLS DPGL  FKDHF YR+KR+ +Q
Sbjct: 105 FPVNKTVQHVLTASAVMTDDSSTKTNIDEDMENIGLLSTDPGLDQFKDHFRYRVKRFIDQ 164

Query: 116 KEQIEKHEGGLDEFSRGYLKFGFNREEGGIVYREWAPAAEEAQVIGDFNGWDGSNHKMEK 175
           K+ IEKHEGGL+EFS+GY KFGFNRE+G IVYREWAPAA+EAQVIGDFNGW+GSNH+MEK
Sbjct: 165 KQLIEKHEGGLEEFSKGYQKFGFNREDGSIVYREWAPAAQEAQVIGDFNGWNGSNHRMEK 224

Query: 176 NEFGVWSIKITDAAGNPAIPHNSRVKFRFKHGNGIWVDRIPAWIRYATVDPSNFGAPYDG 235
           NEFGVWSI+I D+AGNPAIPHNSRVKFRFKHGNG WVDRIPAWIRYATVDPS+FGAPYDG
Sbjct: 225 NEFGVWSIRIPDSAGNPAIPHNSRVKFRFKHGNGAWVDRIPAWIRYATVDPSSFGAPYDG 284

Query: 236 VYWDPPASERYQFEYPRPPKPRAPRIYEAHVGMSSSEPRVNTYREFADDVLPRIRANNYN 295
           VYWDPPASERYQF+YPRPPKPRAPRIYEAHVGMSSSEPRVN+YREFADDVLP I+ANNYN
Sbjct: 285 VYWDPPASERYQFKYPRPPKPRAPRIYEAHVGMSSSEPRVNSYREFADDVLPHIQANNYN 344

Query: 296 TVQLMAVMEHSYYGSFGYHVTNFFAVSSRSGTPEDLKYLIDKAHSLGLCVLMDVIHSHAS 355
           TVQLMAVMEHSYYGSFGYHVTNFFAVSSRSGTPEDLKYLIDKAHSLGL VLMDV+HSHAS
Sbjct: 345 TVQLMAVMEHSYYGSFGYHVTNFFAVSSRSGTPEDLKYLIDKAHSLGLRVLMDVVHSHAS 404

Query: 356 NNVTDGLNGFDVGQSSQESYFHTGDRGYHKLWDSRLFNYANWEVLRFLLSNLRWWLEEYK 415
           NNVTDGLNGFDVGQSSQ+SYFHT DRGYHKLWDSRLFNYANWEVLRFLLSNLRWWLEEYK
Sbjct: 405 NNVTDGLNGFDVGQSSQDSYFHTADRGYHKLWDSRLFNYANWEVLRFLLSNLRWWLEEYK 464

Query: 416 FDGFRFDGVTSMLYHHHGIDMAFTG*LVT*YFSE 449
           FDGFRFDGVTSMLYHHHGI+M F+G  +  YFSE
Sbjct: 465 FDGFRFDGVTSMLYHHHGINMGFSGNYIE-YFSE 497


>27496.m000095 starch branching enzyme II, putative
          Length = 863

 Score =  448 bits (1153), Expect = e-126,   Method: Compositional matrix adjust.
 Identities = 216/351 (61%), Positives = 262/351 (74%), Gaps = 7/351 (1%)

Query: 91  LLSIDPGLGPFKDHFSYRMKRYRNQKEQIEKHEGGLDEFSRGYLKFGFNREEGGIVYREW 150
           +  IDP L  F  H  YR  +Y+  +E+I+K EGGLD FSRGY KFGF R E GI YREW
Sbjct: 160 IYEIDPSLTSFHQHLDYRYSQYKRLREEIDKFEGGLDAFSRGYEKFGFTRSETGITYREW 219

Query: 151 APAAEEAQVIGDFNGWDGSNHKMEKNEFGVWSIKI-TDAAGNPAIPHNSRVKFRFKHGNG 209
           AP A  A +IGDFN W+ +   M +NEFGVW I +  +A G+P IPH SRVK R    +G
Sbjct: 220 APGATWAALIGDFNNWNPNADVMTRNEFGVWEIFLPNNADGSPPIPHGSRVKIRMDTPSG 279

Query: 210 IWVDRIPAWIRYATVDPSNFGAPYDGVYWDPPASERYQFEYPRPPKPRAPRIYEAHVGMS 269
           I  D IPAWI+++   P     PY+G+Y+DPP  E+Y F++ +P +P++ RIYE+HVGMS
Sbjct: 280 I-KDSIPAWIKFSVQAPGEI--PYNGIYYDPPEEEKYVFKHSQPKRPKSLRIYESHVGMS 336

Query: 270 SSEPRVNTYREFADDVLPRIRANNYNTVQLMAVMEHSYYGSFGYHVTNFFAVSSRSGTPE 329
           S+EP +NTY  F DDVLPRI+   YNTVQ+MA+ EHSYY SFGYHVTNFFA SSR GTP+
Sbjct: 337 STEPIINTYANFRDDVLPRIKRLGYNTVQIMAIQEHSYYASFGYHVTNFFAPSSRFGTPD 396

Query: 330 DLKYLIDKAHSLGLCVLMDVIHSHASNNVTDGLNGFDVGQSSQESYFHTGDRGYHKLWDS 389
           DLK LIDKAH L L VLMD++HSH+SNN  DGLN FD    +   YFH+G RGYH +WDS
Sbjct: 397 DLKSLIDKAHELDLLVLMDIVHSHSSNNTLDGLNMFD---GTDGHYFHSGSRGYHWMWDS 453

Query: 390 RLFNYANWEVLRFLLSNLRWWLEEYKFDGFRFDGVTSMLYHHHGIDMAFTG 440
           RLFNY +WEVLRFLLSN RWWL+EYKFDGFRFDGVTSM+Y HHG+++AFTG
Sbjct: 454 RLFNYGSWEVLRFLLSNARWWLDEYKFDGFRFDGVTSMMYTHHGLEVAFTG 504


>29762.m000486 1,4-alpha-glucan branching enzyme, putative
          Length = 894

 Score =  241 bits (614), Expect = 7e-64,   Method: Compositional matrix adjust.
 Identities = 115/251 (45%), Positives = 169/251 (67%), Gaps = 7/251 (2%)

Query: 192 PAIPHNSRVKFRFKHGNGIWVDRIPAWIRYATVDPSNFGAPYDGVYWDPPASERYQFEYP 251
           P IPH S+ +  F   NG  ++R+PAW  Y  V+P   G     ++W+PP    Y+++  
Sbjct: 335 PTIPHGSKYRVYFNTPNGP-LERVPAWATY--VEPGTDGKQPFAIHWEPPPEFAYKWKNT 391

Query: 252 RPPKPRAPRIYEAHVGMSSSEPRVNTYREFADDVLPRIRANNYNTVQLMAVMEHSYYGSF 311
           RP  P++ RIYE HVG+S SEP+++++ +F + VLP ++   YN +QL+ V+EH  Y + 
Sbjct: 392 RPKVPKSLRIYECHVGISGSEPKISSFGDFVEKVLPHVKEAGYNAIQLIGVVEHKDYFTI 451

Query: 312 GYHVTNFFAVSSRSGTPEDLKYLIDKAHSLGLCVLMDVIHSHASNNVTDGLNGFDVGQSS 371
           GY VTN +AVSSR GTP+D K L+D+AH LGL V +D++HS+++ +   GL+ FD    S
Sbjct: 452 GYRVTNLYAVSSRYGTPDDFKRLVDEAHGLGLLVFLDIVHSYSAADEMVGLSLFD---GS 508

Query: 372 QESYFHTGDRGYHKLWDSRLFNYANWEVLRFLLSNLRWWLEEYKFDGFRFDGVTSMLYHH 431
            + YFHTG RG+HK W +R+F Y N EV+ +LLSNL WW+ EY+ DGF+F  ++SM+Y H
Sbjct: 509 NDCYFHTGKRGHHKFWGTRMFKYGNHEVMHYLLSNLNWWVVEYQIDGFQFHSLSSMMYTH 568

Query: 432 HGIDMAFTG*L 442
           +G   +FTG L
Sbjct: 569 NGF-ASFTGDL 578


>28323.m000041 alpha-amylase, putative
          Length = 221

 Score = 55.1 bits (131), Expect = 6e-08,   Method: Compositional matrix adjust.
 Identities = 47/189 (24%), Positives = 84/189 (44%), Gaps = 18/189 (9%)

Query: 249 EYPRPPKPRAPRIYEAHVGMSSSEPRVNTYREFADDVLPRIRANNYNTVQLMAVM---EH 305
           E   P   R   +YE +    S E  +N         LPR+R    + V LM +    + 
Sbjct: 20  EPKHPAWSRGSNVYEVNTRQFSKEGTLNAVAA----SLPRLRQMGVDIVWLMPIHPIGQK 75

Query: 306 SYYGSFG--YHVTNFFAVSSRSGTPEDLKYLIDKAHSLGLCVLMDVIHSHAS------NN 357
           +  G+ G  Y V ++ AV+   GT +D++ L+ +AH+LG+ V++D + +H +        
Sbjct: 76  NRKGTLGSYYAVADYTAVNPEFGTVDDMRKLVRQAHALGMHVILDWVGNHTAWDHPWVQQ 135

Query: 358 VTDGLNGFDVGQSSQESYFHTGDRGYHKLW-DSRLFNYANWEVLRFLLSNLRWWLEEYKF 416
             D     D G+    +     D G    W D    +Y N ++ + +   + +W+++   
Sbjct: 136 HPDWYKKNDKGEIYAVTL--KNDAGGADEWTDVVGLDYDNKDLWKGMTEAMAFWVKDVGV 193

Query: 417 DGFRFDGVT 425
           DGFR D  +
Sbjct: 194 DGFRADAAS 202


>29776.m000488 isoamylase, putative
          Length = 795

 Score = 51.6 bits (122), Expect = 7e-07,   Method: Compositional matrix adjust.
 Identities = 58/228 (25%), Positives = 99/228 (43%), Gaps = 49/228 (21%)

Query: 241 PASERYQFEYPRPPKPRAPR----IYEAHV-GMSSSEPRVNTYREFADDVLPRIRANNY- 294
           P++ + +F++      + P+    IYE HV G +  E   ++  EF    L  +   ++ 
Sbjct: 209 PSASQAEFDWEGDLPLKYPQKDLVIYEMHVRGFTRHE---SSRTEFPGTFLATVEKLDHL 265

Query: 295 -----NTVQLMAV-----MEHSYYGS---------FGYHVTNFFAVSSR---SGTPE--- 329
                N ++LM       +E+  Y S         +GY   N+F+  +R   +GT     
Sbjct: 266 KELGVNCIELMPCHEFNELEYYSYNSVLGDYKMNFWGYSTINYFSPMTRYSSAGTRNCGR 325

Query: 330 ----DLKYLIDKAHSLGLCVLMDVIHSHASNNVTDG----LNGFDVGQSSQESYFHTGDR 381
               + K+L+ +AH  G+ V+MDV+ +H +     G      G D        Y+    +
Sbjct: 326 DAINEFKFLVREAHKRGIEVIMDVVFNHTAEGNQKGPILSFRGVD-----NSVYYMLAPK 380

Query: 382 G--YHKLWDSRLFNYANWEVLRFLLSNLRWWLEEYKFDGFRFDGVTSM 427
           G  Y+       FN  +  V +F+L  LR+W+ E   DGFRFD  + M
Sbjct: 381 GEFYNYSGCGNTFNCNHPIVRQFILDCLRYWVLEMHVDGFRFDLASIM 428


>29908.m006089 isoamylase, putative
          Length = 783

 Score = 50.4 bits (119), Expect = 2e-06,   Method: Compositional matrix adjust.
 Identities = 46/190 (24%), Positives = 77/190 (40%), Gaps = 35/190 (18%)

Query: 269 SSSEPRVNTYREFADDVLPRIRANNYNTVQLMAVMEHSYY-------------GSFGYHV 315
           S  EP++        + +P +     N V+L+ V E   +              ++GY  
Sbjct: 268 SGLEPKIRGSYLGVIEKIPHLLELGVNAVELLPVFEFDEFELQRRPNPRDHMINTWGYST 327

Query: 316 TNFFAVSSR----SGTP----EDLKYLIDKAHSLGLCVLMDVIHSHAS-----NNVTDGL 362
            NFFA  SR     G P     + K ++   H  G+ V++DV+++H +     N  T   
Sbjct: 328 INFFAPMSRYASGGGGPCNASREFKEMVKALHGAGIEVILDVVYNHTNEADDKNPYTTSF 387

Query: 363 NGFDVGQSSQESYFHTGDRGYHKLWD----SRLFNYANWEVLRFLLSNLRWWLEEYKFDG 418
            G D        Y+       ++L +        N  +  V+  +L +LR W+ EY  DG
Sbjct: 388 RGID-----NMIYYMLDLNNKNQLLNFSGCGNTLNCNHPVVMELILESLRHWVTEYHVDG 442

Query: 419 FRFDGVTSML 428
           FRFD  + + 
Sbjct: 443 FRFDLASVLC 452