Jatropha Genome Database

JcCB0030781.10
Show Alignment:  
BLASTP 2.2.23 [Feb-03-2010]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Reference for compositional score matrix adjustment: Altschul, Stephen F., 
John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis,
Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches
using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.

Query= JcCB0030781.10 - phase: 0 /partial
         (268 letters)

Database: castor_wgs_0.1_aa 
           31,221 sequences; 10,419,776 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

29776.m000488 isoamylase, putative                                    402   e-112
29908.m006089 isoamylase, putative                                    162   2e-40
28153.m000287 isoamylase, putative                                     82   3e-16

>29776.m000488 isoamylase, putative
          Length = 795

 Score =  402 bits (1032), Expect = e-112,   Method: Compositional matrix adjust.
 Identities = 188/238 (78%), Positives = 200/238 (84%)

Query: 3   SMNQEGGRKPWNSINFVCAHDGFTLADLVTYXXXXXXXXXXXXXXXXXXXXXXXCGLEGE 62
           ++ QEGGRKPWNSINF+CAHDGFTLADLV+Y                       CG EGE
Sbjct: 532 NLYQEGGRKPWNSINFICAHDGFTLADLVSYNNKNNLANGEDNNDGESHNNSWNCGQEGE 591

Query: 63  FASISVKKLRKRQMRNFFVCLMVSQGVPMIYMGDEYGHTKGGNNNTYCHDNYINYFRWDK 122
           FASI VKKLRKRQMRNFFVCLMVSQGVPMIYMGDEYGHTKGGNNNTYCHDNYINYFRWDK
Sbjct: 592 FASILVKKLRKRQMRNFFVCLMVSQGVPMIYMGDEYGHTKGGNNNTYCHDNYINYFRWDK 651

Query: 123 KEESSSDFYRFCCLMTKFRSECESLGLNDFPTAERLQWHGHVPRMPDWSETSRFVAFTVT 182
           KEESSSDFYRFC LMTKFR ECESLGLNDFPTAERLQWHGH P MPDWSETSRFVAFT+ 
Sbjct: 652 KEESSSDFYRFCRLMTKFRHECESLGLNDFPTAERLQWHGHSPGMPDWSETSRFVAFTLN 711

Query: 183 DTVKGEIYVAFNAKHLPVTVALPERLGYRWEPLVDTGKASPFDFLSGDVPEREIAIKQ 240
           D+VKGEIYVAFNA HLPVT+ LPER GYRW+PLVDTGK +PFDF S D+P+R+IA KQ
Sbjct: 712 DSVKGEIYVAFNANHLPVTIGLPERPGYRWQPLVDTGKPAPFDFFSSDIPDRDIAYKQ 769


>29908.m006089 isoamylase, putative
          Length = 783

 Score =  162 bits (409), Expect = 2e-40,   Method: Compositional matrix adjust.
 Identities = 96/253 (37%), Positives = 132/253 (52%), Gaps = 19/253 (7%)

Query: 10  RKPWNSINFVCAHDGFTLADLVTYXXXXXXXXXXXXXXXXXXXXXXXCGLEGEFASISVK 69
           RKP++S+NFV AHDGFTL DLV+Y                       CG EGE    ++K
Sbjct: 543 RKPFHSVNFVIAHDGFTLHDLVSYNFKHNDANGEGGNDGSNDNFSWNCGFEGETDDPNIK 602

Query: 70  KLRKRQMRNFFVCLMVSQGVPMIYMGDEYGHTKGGNNNTYCHDNYINYFRWDKKEESSSD 129
            LR RQM+NF + LM+SQG PM+ MGDEYGHT+ GNNN+Y HD  IN F+W+      +D
Sbjct: 603 ALRSRQMKNFHLALMISQGTPMMLMGDEYGHTRYGNNNSYGHDTSINNFQWELLAAQRND 662

Query: 130 FYRFCCLMTKFRSECESLGLNDFPTAERLQWHGHVPRMPDWSE-TSRFVAFTVTDTVKGE 188
            ++F   + KFR   +    ++F     + WH       +W    S+F+AFT+ ++   +
Sbjct: 663 HFQFFSEVIKFRRTHQVFRHDNFLNQNDVTWHED-----NWDNYESKFLAFTLHESNGAD 717

Query: 189 IYVAFNAKHLPVTVALPERLGYR-WEPLVDTGKASPFDFLSGDVPEREIAIKQYSHFLDA 247
           IY+AFNA    + V +P     R W  + DT   SP DF+   VP    A          
Sbjct: 718 IYLAFNAHDYFIKVLIPPPPSKRSWFRVADTNLKSPDDFVPEGVPGIGSA---------- 767

Query: 248 NLYPMHSYSSIIL 260
             Y +  YSSI+L
Sbjct: 768 --YNVAPYSSILL 778


>28153.m000287 isoamylase, putative
          Length = 872

 Score = 82.0 bits (201), Expect = 3e-16,   Method: Compositional matrix adjust.
 Identities = 73/261 (27%), Positives = 107/261 (40%), Gaps = 46/261 (17%)

Query: 9   GRKPWNSINFVCAHDGFTLADLVTYXXXXXXXXXXXXXXXXXXXXXXXCGLEGEFASISV 68
           GR P  S N++  + G  L DLV++                       CG EG     +V
Sbjct: 620 GRGPAFSFNYIARNSGLPLVDLVSFSGGELGSELSWN-----------CGEEGPTNKTAV 668

Query: 69  KKLRKRQMRNFFVCLMVSQGVPMIYMGDEYGHTKGGNNNTYCHDNYINY-----FRWDKK 123
            + R +Q+RN+   L VS GVP++ MGDE G +  G+         I+Y     F W+  
Sbjct: 669 LERRLKQIRNYLFILYVSLGVPVLNMGDECGQSSRGS---------ISYGDRKPFDWNAL 719

Query: 124 EES-SSDFYRFCCLMTKFR-SECESLGLNDFPTAERLQWHGHVPRMPDWSE-TSRFVAFT 180
             S  +   +F   ++  R    + L   +F   E + WHG+    P W + T +F+A T
Sbjct: 720 STSFGNQMTQFISFLSSLRMRRSDLLQKRNFLKEENIDWHGNDQSPPRWEDPTCKFLAMT 779

Query: 181 VT------------DTVKGEIYVAFNAK-HLPVTVALPERLGYRWEPLVDTGKASPFDFL 227
           +               +KG++++AFNA  H    +  P   G  W  LVDT    P  F 
Sbjct: 780 LKIDKAESQLSSEPSNIKGDLFMAFNAAGHAESVILPPVPEGMIWRRLVDTALPFPGFFS 839

Query: 228 SGDVPERE-----IAIKQYSH 243
               P  E     IA K  SH
Sbjct: 840 EDGEPVVEQIAGLIAYKMNSH 860