Jatropha Genome Database

JcCA0300271.10
Show Alignment:  
BLASTP 2.2.23 [Feb-03-2010]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Reference for compositional score matrix adjustment: Altschul, Stephen F., 
John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis,
Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches
using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.

Query= JcCA0300271.10 + phase: 0 /partial
         (489 letters)

Database: castor_wgs_0.1_aa 
           31,221 sequences; 10,419,776 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

30143.m001200 folylpolyglutamate synthase, putative                   745   0.0  
28470.m000437 folylpolyglutamate synthase, putative                   435   e-122
30169.m006535 tetrahydrofolylpolyglutamate synthase, putative         109   3e-24

>30143.m001200 folylpolyglutamate synthase, putative
          Length = 531

 Score =  745 bits (1923), Expect = 0.0,   Method: Compositional matrix adjust.
 Identities = 363/478 (75%), Positives = 393/478 (82%), Gaps = 12/478 (2%)

Query: 1   EMKIIHVAGTKGKGSTCTFTESILRNCGFHTGLFTSPHLIDIRERFRLDGGDICEEKFLA 60
           EMKIIHVAGTKGKGSTCTFTESILRNCGF TGLFTSPHLID+RERFRLDG DICEEKFLA
Sbjct: 60  EMKIIHVAGTKGKGSTCTFTESILRNCGFRTGLFTSPHLIDVRERFRLDGEDICEEKFLA 119

Query: 61  YFWWCYDRLKEKTTEDIPMPTYXXXXXXXXXXXXXXEQVDVAILEVGLGGRFDATNVVQT 120
           YFWWCYDRLKEKTTE+IPMPTY              EQVDVAILEVGLGG+FDATNVVQT
Sbjct: 120 YFWWCYDRLKEKTTEEIPMPTYFRFLALLAFKIFAAEQVDVAILEVGLGGKFDATNVVQT 179

Query: 121 PIVCGIASLGYDHMEILGNTLGEIAGEKAGIFKHGIPAFTVPQPDEAMRVLEEKASKLDV 180
           PIVCGI+SLGYDHMEILGNTLGEIAGEKAGIFKH IPAFTVPQ DEAM VLE+KASKLDV
Sbjct: 180 PIVCGISSLGYDHMEILGNTLGEIAGEKAGIFKHRIPAFTVPQLDEAMHVLEDKASKLDV 239

Query: 181 PLQVAPPLDSXXXXXXXXXXXXXHQYINAGLAIALASKWLQRTGHLEVTYFEQNNSLPEQ 240
           PLQVAPPLD              HQY+NAGLAIAL+SKWLQRTGH+E +Y EQ++ LPE 
Sbjct: 240 PLQVAPPLDPSLLNGLKLGLEGEHQYLNAGLAIALSSKWLQRTGHVEFSYLEQSSPLPEH 299

Query: 241 FIKGLTTACLQGRAQIVPDRYINTECDGDLVFYLDGAHSPESMEACARWFSIAIKDSNQQ 300
           FI+GLTTACLQGRAQIVPDR +NTE +GDLVFYLDGAHSPESMEACARWFS+AIKD NQQ
Sbjct: 300 FIRGLTTACLQGRAQIVPDRCVNTETNGDLVFYLDGAHSPESMEACARWFSLAIKDDNQQ 359

Query: 301 STFNCPSQNNYQPATELVEKHHDGRPKSNFMQILLFNCMSVRDPQLLLPRLMKTCASHGV 360
            T N P   NY+        H         MQILLFNCMSVRDPQLLLPRLMKTCAS GV
Sbjct: 360 HTLNSPPHRNYESTLGF---HLS-------MQILLFNCMSVRDPQLLLPRLMKTCASQGV 409

Query: 361 SFKKALFVPNVSVYYKVGSQSLPQTD--VDLSWQFTSQKLWENLVRGDKGGEANNAGPVC 418
            FKKALFVPN SVYYKVGSQ+LP  D  VDLSWQFT Q+LWE+LVRGDKGGEANN   +C
Sbjct: 410 YFKKALFVPNTSVYYKVGSQALPPVDPQVDLSWQFTLQRLWESLVRGDKGGEANNVDALC 469

Query: 419 EEVKDDTGTSVRTCENSAVFPSLPLAIKWLRDNIHQNRSVRIQVMLINTFCFILYILH 476
           EEVKD+TG +V+ CENSAVFPSLPLAIKWLRD++ QNRSVRIQV++  +   +  +L 
Sbjct: 470 EEVKDETGATVKNCENSAVFPSLPLAIKWLRDSVLQNRSVRIQVLVTGSLHLVGDVLR 527


>28470.m000437 folylpolyglutamate synthase, putative
          Length = 581

 Score =  435 bits (1119), Expect = e-122,   Method: Compositional matrix adjust.
 Identities = 231/427 (54%), Positives = 284/427 (66%), Gaps = 26/427 (6%)

Query: 2   MKIIHVAGTKGKGSTCTFTESILRNCGFHTGLFTSPHLIDIRERFRLDGGDICEEKFLAY 61
           +KIIHVAGTKGKGSTCTF+E+ILR CGF TGLFTSPHLID+RERFR+DG DI E+KFL Y
Sbjct: 133 LKIIHVAGTKGKGSTCTFSEAILRQCGFRTGLFTSPHLIDVRERFRIDGLDISEDKFLLY 192

Query: 62  FWWCYDRLKEKTTEDIPMPTYXXXXXXXXXXXXXXEQVDVAILEVGLGGRFDATNVVQTP 121
           FW C+++LK K TED+PMP                EQVDVA++EVGLGG  D+TNV++ P
Sbjct: 193 FWDCWNQLKGKVTEDLPMPPLFQFLTVLAFKIFICEQVDVAVIEVGLGGTDDSTNVIKEP 252

Query: 122 IVCGIASLGYDHMEILGNTLGEIAGEKAGIFKHGIPAFTVPQPDEAMRVLEEKASKLDVP 181
           +VCGI SLG DH E LGNTLG+IA  KAGIFK  IPAFTVPQ  EAM VL+ KA +L V 
Sbjct: 253 VVCGITSLGMDHTESLGNTLGKIASHKAGIFKPQIPAFTVPQLSEAMDVLQGKAHELMVS 312

Query: 182 LQVAPPLDSXXXXXXXXXXXXXHQYINAGLAIALASKWLQRTGHLEVTYFEQNN---SLP 238
           LQV  PLD              HQ+ NAGLA++L   WLQRTG+ E   F+ NN    LP
Sbjct: 313 LQVVTPLDYKKWEGLKLSLSGDHQFTNAGLAVSLCKCWLQRTGNWE-KIFQNNNQKADLP 371

Query: 239 EQFIKGLTTACLQGRAQIVPD---RY-----INTECDGDLVFYLDGAHSPESMEACARWF 290
           E F +GL TA L GRAQIV D   R+     +     G+L+FYLDGAHSPESMEACA+WF
Sbjct: 372 EAFRRGLATAHLSGRAQIVYDSSSRFHSSLEVAESSSGELIFYLDGAHSPESMEACAKWF 431

Query: 291 SIAIKDSNQQSTFNCPSQNNYQPATELV-------EKHHDGRPKSNFMQILLFNCMSVRD 343
           S A++++      + P  N  Q   E++       E+      K    +ILLFNCM VRD
Sbjct: 432 SSAVQENKTLPELSSP--NGAQVMKEVLGNGYIQCERSSAEESKKTSKKILLFNCMDVRD 489

Query: 344 PQLLLPRLMKTCASHGVSFKKALFVPNVSVYYKV--GSQSLPQTDV---DLSWQFTSQKL 398
           P++LLP+L+ TCAS G  F KA+FVP++S Y KV  G+ ++P       DLSWQF+ QKL
Sbjct: 490 PKILLPQLVSTCASSGTYFSKAIFVPSMSTYNKVTSGASAIPSDTSPTRDLSWQFSLQKL 549

Query: 399 WENLVRG 405
           W+ ++ G
Sbjct: 550 WQKIIHG 556


>30169.m006535 tetrahydrofolylpolyglutamate synthase, putative
          Length = 552

 Score =  109 bits (272), Expect = 3e-24,   Method: Compositional matrix adjust.
 Identities = 88/311 (28%), Positives = 133/311 (42%), Gaps = 26/311 (8%)

Query: 1   EMKIIHVAGTKGKGSTCTFTESILRNCGFHTGLFTSPHLIDIRERFRLD--GGDICEEKF 58
           + K +H+AGTKGKGST  F  +ILR  G+  G +TSPH++ IRER  +   G  +     
Sbjct: 90  KFKAVHIAGTKGKGSTAAFLSNILRAQGYSVGCYTSPHIVTIRERMSIGKLGKPVSPMAL 149

Query: 59  LAYFWWCYDRLKEKTTEDIPMPTYXXXXXXXXXXXXXXEQVDVAILEVGLGGRFDATNVV 118
            ++F     +L E    +    ++              E VD+AI+E GLGG  DATN++
Sbjct: 150 NSHFHQIRQKLDEAIQLENGCLSHFEVLTAIAFSLFAQENVDIAIIEAGLGGARDATNIL 209

Query: 119 QTPIVCG--IASLGYDHMEILGNTLGEIAGEKAGIFKHGIP-AFTVPQPDEAMRVLEEKA 175
            +  +    I ++G +H+  LG +L  IA  KAGI K G P     P   +  R+L +KA
Sbjct: 210 CSSQLATSIITTIGEEHLMALGGSLESIAMAKAGIIKRGRPLILGGPFLPDIERILRDKA 269

Query: 176 SKLDVPLQVAPPLDSXXXXXXXXXXXXXH-QYINAGLAIALASKWLQRTGHLEVTYF--- 231
           S +  P+  A                  H Q  +  + +  A         LE+      
Sbjct: 270 SLMLAPVVSASDSGIRTTIKGLSMFDGRHCQLCDIKIRVKKALNLFIELSDLEMRMLGSH 329

Query: 232 ----------------EQNNSLPEQFIK-GLTTACLQGRAQIVPDRYINTECDGDLVFYL 274
                           +Q   + +  I+ GL   CL GR+Q +  +             L
Sbjct: 330 QLQNAATAACAALCLRDQGWKISDASIRLGLENTCLLGRSQFLASKDAEVLGVPGATILL 389

Query: 275 DGAHSPESMEA 285
           DGAH+ ES +A
Sbjct: 390 DGAHTKESAKA 400