The cDNA fragment was inserted at the NotI-SalI site of the pBluescript II SK(+) vector (GenBank Accession No. X52328, AmpR). The 5' end of the cDNA was ligated at the NotI site and the 3' end of the cDNA was inserted at the SalI site of the vector. The adjacent sequences to the cDNA are as follows: AACCCTCACTAAAGGGAACAAAAGCTGGAGCTCCACCGCGGTG-5' end of cDNA, GGGCCCCCCCTCGAGGTCGACCCACGCGTCCGTTTTTTTTTTTTTTT-3' end of cDNA. |
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