##################################
# NO CUT ENZYMES ( 60 enzymes) #
##################################
AatII AccI AclI AgeI Aor51HI
ApaI AscI AspI AviII AvrII
BbeI BbrPI BcnI BlnI BsiEI
BsiWI BsmBI BspDI BssHII Bst1107I
BstZ17I ClaI CpoI EagI EclXI
Eco47III Eco52I EcoRV FseI FspI
KasI KspI MluI NarI NciI
NcoI NheI NotI NruI PflFI
PinAI PmaCI PmeI PmlI Psp1406I
PvuI RsrII SacII SalI SbfI
SfiI SfoI SgrAI SmaI SphI
Sse8387I StuI Tth111I XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 59 enzymes) #
###################################
Acc65I 1656 GGTACC G^GTACC
AccII 1454 CGCG CG^CG
AccIII 2289 TCCGGA T^CCGGA
AcyI 3616 GACGCC G[GA]^CG[CT]C
AhdI 829 GACCAGTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 3463 GTGCAC G^TGCAC
AlwNI 3547 CAGTATCTG CAG[ACGT][ACGT][ACGT]^CTG
ApaLI 3463 GTGCAC G^TGCAC
Asp718I 1656 GGTACC G^GTACC
AspEI 829 GACCAGTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BcgI 2026 GCAGTACCATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 3945 GGATAC GTATCC(6/5)
BglI 388 GCCATGAGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlpI 264 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 264 GCTCAGC GC^T[ACGT]AGC
BsaBI 1846 GATCATTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI 3616 GACGCC G[GA]^CG[CT]C
BseAI 2289 TCCGGA T^CCGGA
BsmI 4076 GCATTC GAATGC(1/-1)
Bsp1407I 1228 TGTACA T^GTACA
BspEI 2289 TCCGGA T^CCGGA
BspHI 4493 TCATGA T^CATGA
BsrFI 2144 GCCGGC [GA]^CCGG[CT]
BsrGI 1228 TGTACA T^GTACA
BstBI 2 TTCGAA TT^CGAA
BstEII 696 GGTCACC G^GT[ACGT]ACC
BstPI 696 GGTCACC G^GT[ACGT]ACC
BstUI 1454 CGCG CG^CG
CelII 264 GCTCAGC GC^T[ACGT]AGC
Cfr10I 2144 GCCGGC [GA]^CCGG[CT]
DrdI 4462 GACTTAACTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eam1105I 829 GACCAGTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoO65I 696 GGTCACC G^GT[ACGT]ACC
EcoT22I 3512 ATGCAT ATGCA^T
FauI 1793 CCCGC CCCGC(4/6)
HaeII 1280 AGCGCC [GA]GCGC^[CT]
HgaI 3616 GACGC GACGC(5/10)
Hin1I 3616 GACGCC G[GA]^CG[CT]C
HpaI 4839 GTTAAC GTT^AAC
KpnI 1656 GGTACC GGTAC^C
MamI 1846 GATCATTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MroI 2289 TCCGGA T^CCGGA
MvnI 1454 CGCG CG^CG
NaeI 2144 GCCGGC GCC^GGC
NgoMIV 2144 GCCGGC G^CCGGC
NsiI 3512 ATGCAT ATGCA^T
NspV 2 TTCGAA TT^CGAA
PacI 4572 TTAATTAA TTAAT^TAA
PflMI 470 CCAATCTTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
RcaI 4493 TCATGA T^CATGA
SapI 2498 GAAGAGC GCTCTTC(1/4)
ScaI 3847 AGTACT AGT^ACT
SexAI 3017 ACCAGGT A^CC[AT]GGT
SfuI 2 TTCGAA TT^CGAA
SnaBI 3948 TACGTA TAC^GTA
SpeI 360 ACTAGT A^CTAGT
SspBI 1228 TGTACA T^GTACA
SspI 4500 AATATT AAT^ATT
Van91I 470 CCAATCTTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
###################################
# TWO CUT ENZYMES ( 39 enzymes) #
###################################
AflII 3419 CTTAAG C^TTAAG
4376 CTTAAG
AocI 2629 CCTCAGG CC^T[ACGT]AGG
3265 CCTGAGG
AseI 3508 ATTAAT AT^TAAT
3672 ATTAAT
AsnI 3508 ATTAAT AT^TAAT
3672 ATTAAT
Asp700I 1258 GAAAAATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1549 GAATCTGTTC
AvaI 1661 CTCGAG C^[CT]CG[GA]G
1715 CTCGAG
BalI 27 TGGCCA TGG^CCA
2780 TGGCCA
BclI 4131 TGATCA T^GATCA
4396 TGATCA
BfrI 3419 CTTAAG C^TTAAG
4376 CTTAAG
BglII 1617 AGATCT A^GATCT
2487 AGATCT
BsaAI 2876 TACGTG [CT]AC^GT[GA]
3948 TACGTA
BsaWI 825 ACCGGA [AT]^CCGG[AT]
2289 TCCGGA
BsoBI 1661 CTCGAG C^[CT]CG[GA]G
1715 CTCGAG
BspLU11I 1178 ACATGT A^CATGT
4169 ACATGT
BsrBI 262 CCGCTC CCGCTC(-3/-3)
3424 GAGCGG
BssSI 145 CTCGTG CACGAG(-5/-1)
1214 CTCGTG
Bsu36I 2629 CCTCAGG CC^T[ACGT]AGG
3265 CCTGAGG
CfoI 1281 GCGC GCG^C
5168 GCGC
DsaI 622 CCACGG C^C[GA][CT]GG
2685 CCACGG
Eco81I 2629 CCTCAGG CC^T[ACGT]AGG
3265 CCTGAGG
EcoNI 3252 CCTGTCAGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
3265 CCTGAGGGAGG
EcoRI 3820 GAATTC G^AATTC
3909 GAATTC
FbaI 4131 TGATCA T^GATCA
4396 TGATCA
HhaI 1281 GCGC GCG^C
5168 GCGC
HinP1I 1281 GCGC G^CGC
5168 GCGC
MfeI 941 CAATTG C^AATTG
2842 CAATTG
MluNI 27 TGGCCA TGG^CCA
2780 TGGCCA
MscI 27 TGGCCA TGG^CCA
2780 TGGCCA
MunI 941 CAATTG C^AATTG
2842 CAATTG
NdeI 874 CATATG CA^TATG
2675 CATATG
PaeR7I 1661 CTCGAG C^TCGAG
1715 CTCGAG
PshAI 342 GACTCTAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
1018 GACCCTAGTC
PshBI 3508 ATTAAT AT^TAAT
3672 ATTAAT
PstI 139 CTGCAG CTGCA^G
2021 CTGCAG
SwaI 4121 ATTTAAAT ATTT^AAAT
5071 ATTTAAAT
XbaI 2178 TCTAGA T^CTAGA
3925 TCTAGA
XcmI 2414 CCAAAGATGTTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
2962 CCACCTCCAAAATGG
XhoI 1661 CTCGAG C^TCGAG
1715 CTCGAG
XmnI 1258 GAAAAATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1549 GAATCTGTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 100 enzymes) #
#########################################
AciI AcsI AfaI AflIII AluI
AlwI ApoI AspHI AvaII BamHI
BanI BanII BbsI BbvCI BbvI
BfaI BmyI BpmI BpuAI BsaI
BsaJI BseRI BsgI BsiHKAI BsiYI
BslI BsmAI BsmFI Bsp1286I BspMI
BsrDI BsrI BssKI BstAPI BstF5I
BstNI BstXI BstYI Cac8I Cfr13I
DdeI DpnII DraI DraII DraIII
EaeI EarI EcoO109I EcoRII EcoT14I
Fnu4HI FokI HaeIII HapII HincII
HindII HindIII HinfI HpaII HphI
ItaI Ksp632I MaeI MaeII MaeIII
MboI MboII MflI MnlI MseI
MslI MspA1I MspI MvaI MwoI
NdeII NlaIII NlaIV NspI PleI
PpuMI PvuII RsaI SacI Sau3AI
Sau96I ScrFI SfaNI SfcI SmlI
StyI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I XhoII