################################## # NO CUT ENZYMES ( 71 enzymes) # ################################## AatII Acc65I AccIII AflII AflIII AgeI AhdI AscI Asp718I AspEI AviII BamHI BbrPI BcgI BclI BfrI BglII BsaBI BseAI BsiEI BsiWI BspDI BspEI BspLU11I BssHII Bst1107I BstBI BstZ17I ClaI CpoI DrdI EagI Eam1105I EclXI Eco52I EcoRV FbaI FspI HincII HindII HpaI KpnI KspI MamI MfeI MluI MroI MunI NheI NotI NruI NspV PacI PinAI PmaCI PmeI PmlI PvuI RsrII SacII SalI SbfI ScaI SexAI SfuI SnaBI SpeI SphI Sse8387I SwaI XbaI ################################### # ONE CUT ENZYMES ( 33 enzymes) # ################################### AclI 5373 AACGTT AA^CGTT Alw44I 3569 GTGCAC G^TGCAC ApaLI 3569 GTGCAC G^TGCAC AseI 5333 ATTAAT AT^TAAT AsnI 5333 ATTAAT AT^TAAT BbeI 1033 GGCGCC GGCGC^C BciVI 2498 GGATAC GTATCC(6/5) BsaAI 5724 CACGTA [CT]AC^GT[GA] BsaWI 1511 ACCGGA [AT]^CCGG[AT] BsmI 1218 GAATGC GAATGC(1/-1) BspHI 4689 TCATGA T^CATGA DraI 386 TTTAAA TTT^AAA DraIII 231 CACGAAGTG CAC[ACGT][ACGT][ACGT]^GTG EcoRI 5821 GAATTC G^AATTC EcoT22I 66 ATGCAT ATGCA^T FseI 1698 GGCCGGCC GGCCGG^CC HindIII 358 AAGCTT A^AGCTT KasI 1033 GGCGCC G^GCGCC NarI 1033 GGCGCC GG^CGCC NsiI 66 ATGCAT ATGCA^T PaeR7I 1643 CTCGAG C^TCGAG PshBI 5333 ATTAAT AT^TAAT Psp1406I 5373 AACGTT AA^CGTT RcaI 4689 TCATGA T^CATGA SacI 4455 GAGCTC GAGCT^C SapI 2436 GAAGAGC GCTCTTC(1/4) SfiI 1739 GGCCCCAGAGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC SfoI 1033 GGCGCC GGC^GCC SgrAI 1786 CACCGGCG C[GA]^CCGG[CT]G SmaI 2007 CCCGGG CCC^GGG XhoI 1643 CTCGAG C^TCGAG XmaCI 2007 CCCGGG C^CCGGG XmaI 2007 CCCGGG C^CCGGG ################################### # TWO CUT ENZYMES ( 32 enzymes) # ################################### AccII 741 CGCG CG^CG 2442 CGCG AcyI 1033 GGCGCC G[GA]^CG[CT]C 1995 GACGCC Aor51HI 720 AGCGCT AGC^GCT 1392 AGCGCT AspHI 3569 GTGCAC G[AT]GC[AT]^C 4455 GAGCTC AspI 2050 GACTTCGTC GAC[ACGT]^[ACGT][ACGT]GTC 4660 GACAAAGTC AvrII 3875 CCTAGG C^CTAGG 4190 CCTAGG BlnI 3875 CCTAGG C^CTAGG 4190 CCTAGG BsaHI 1033 GGCGCC G[GA]^CG[CT]C 1995 GACGCC BsiHKAI 3569 GTGCAC G[AT]GC[AT]^C 4455 GAGCTC BsmBI 5601 CGTCTC CGTCTC(1/5) 2462 GAGACG Bsp1407I 5431 TGTACA T^GTACA 5554 TGTACA BspMI 4889 GCAGGT ACCTGC(4/8) 4989 GCAGGT BsrDI 1587 GCAATG GCAATG(2/0) 5760 GCAATG BsrGI 5431 TGTACA T^GTACA 5554 TGTACA BssSI 1497 CACGAG CACGAG(-5/-1) 4474 CACGAG BstUI 741 CGCG CG^CG 2442 CGCG Eco47III 720 AGCGCT AGC^GCT 1392 AGCGCT HgaI 1995 GACGC GACGC(5/10) 5600 GCGTC Hin1I 1033 GGCGCC G[GA]^CG[CT]C 1995 GACGCC MvnI 741 CGCG CG^CG 2442 CGCG NaeI 1699 GCCGGC GCC^GGC 1766 GCCGGC NcoI 1228 CCATGG C^CATGG 3090 CCATGG NdeI 2665 CATATG CA^TATG 5502 CATATG NgoMIV 1699 GCCGGC G^CCGGC 1766 GCCGGC PflFI 2050 GACTTCGTC GAC[ACGT]^[ACGT][ACGT]GTC 4660 GACAAAGTC PshAI 990 GACCTTGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC 1845 GACCAAGGTC PstI 526 CTGCAG CTGCA^G 830 CTGCAG SfcI 526 CTGCAG C^T[GA][CT]AG 830 CTGCAG SspBI 5431 TGTACA T^GTACA 5554 TGTACA SspI 5260 AATATT AAT^ATT 5325 AATATT StuI 1130 AGGCCT AGG^CCT 3505 AGGCCT Tth111I 2050 GACTTCGTC GAC[ACGT]^[ACGT][ACGT]GTC 4660 GACAAAGTC ######################################### # 3 OR MORE CUT ENZYMES ( 122 enzymes) # ######################################### AccI AciI AcsI AfaI AluI AlwI AlwNI AocI ApaI ApoI Asp700I AvaI AvaII BalI BanI BanII BbsI BbvCI BbvI BcnI BfaI BglI BlpI BmyI BpmI Bpu1102I BpuAI BsaI BsaJI BseRI BsgI BsiYI BslI BsmAI BsmFI BsoBI Bsp1286I BsrBI BsrFI BsrI BssKI BstAPI BstEII BstF5I BstNI BstPI BstXI BstYI Bsu36I Cac8I CelII CfoI Cfr10I Cfr13I DdeI DpnII DraII DsaI EaeI EarI Eco81I EcoNI EcoO109I EcoO65I EcoRII EcoT14I FauI Fnu4HI FokI HaeII HaeIII HapII HhaI HinP1I HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MluNI MnlI MscI MseI MslI MspA1I MspI MvaI MwoI NciI NdeII NlaIII NlaIV NspI PflMI PleI PpuMI PvuII RsaI Sau3AI Sau96I ScrFI SfaNI SmlI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I Van91I XcmI XhoII XmnI