##################################
# NO CUT ENZYMES ( 43 enzymes) #
##################################
AclI AflII AgeI AhdI AscI
AspEI AviII BfrI BsaBI BsiWI
BsmBI BspDI BssSI Bst1107I BstZ17I
ClaI CpoI Eam1105I EcoRV FseI
FspI HpaI MamI MfeI MluI
MunI NheI NotI NruI PacI
PaeR7I PinAI PmeI Psp1406I PvuI
RsrII SalI SbfI SfiI SgrAI
Sse8387I SwaI XhoI
###################################
# ONE CUT ENZYMES ( 23 enzymes) #
###################################
AatII 901 GACGTC GACGT^C
Aor51HI 9629 AGCGCT AGC^GCT
AseI 7470 ATTAAT AT^TAAT
AsnI 7470 ATTAAT AT^TAAT
AvrII 5107 CCTAGG C^CTAGG
BbrPI 7258 CACGTG CAC^GTG
BcgI 749 GCAGGTCAATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BlnI 5107 CCTAGG C^CTAGG
BsiEI 5 CGGCCG CG[GA][CT]^CG
BsrDI 2484 CATTGC GCAATG(2/0)
EagI 5 CGGCCG C^GGCCG
EclXI 5 CGGCCG C^GGCCG
Eco47III 9629 AGCGCT AGC^GCT
Eco52I 5 CGGCCG C^GGCCG
KspI 169 CCGCGG CCGC^GG
PmaCI 7258 CACGTG CAC^GTG
PmlI 7258 CACGTG CAC^GTG
PshBI 7470 ATTAAT AT^TAAT
SacII 169 CCGCGG CCGC^GG
SmaI 264 CCCGGG CCC^GGG
SnaBI 9926 TACGTA TAC^GTA
XmaCI 264 CCCGGG C^CCGGG
XmaI 264 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 27 enzymes) #
###################################
AccI 5643 GTCTAC GT^[AC][GT]AC
8611 GTCTAC
Alw44I 1225 GTGCAC G^TGCAC
1585 GTGCAC
ApaLI 1225 GTGCAC G^TGCAC
1585 GTGCAC
AspI 4205 GACTTTGTC GAC[ACGT]^[ACGT][ACGT]GTC
7859 GACTTAGTC
BbeI 101 GGCGCC GGCGC^C
3161 GGCGCC
BlpI 357 GCTGAGC GC^T[ACGT]AGC
7545 GCTCAGC
Bpu1102I 357 GCTGAGC GC^T[ACGT]AGC
7545 GCTCAGC
BspHI 935 TCATGA T^CATGA
8891 TCATGA
BssHII 66 GCGCGC G^CGCGC
820 GCGCGC
BstBI 1910 TTCGAA TT^CGAA
6813 TTCGAA
BstEII 3552 GGTCACC G^GT[ACGT]ACC
6924 GGTCACC
BstPI 3552 GGTCACC G^GT[ACGT]ACC
6924 GGTCACC
CelII 357 GCTGAGC GC^T[ACGT]AGC
7545 GCTCAGC
DrdI 901 GACGTCCACGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
2830 GACAACAGCGTC
EcoO65I 3552 GGTCACC G^GT[ACGT]ACC
6924 GGTCACC
KasI 101 GGCGCC G^GCGCC
3161 GGCGCC
NaeI 3 GCCGGC GCC^GGC
139 GCCGGC
NarI 101 GGCGCC GG^CGCC
3161 GGCGCC
NdeI 1562 CATATG CA^TATG
8736 CATATG
NgoMIV 3 GCCGGC G^CCGGC
139 GCCGGC
NspV 1910 TTCGAA TT^CGAA
6813 TTCGAA
PflFI 4205 GACTTTGTC GAC[ACGT]^[ACGT][ACGT]GTC
7859 GACTTAGTC
RcaI 935 TCATGA T^CATGA
8891 TCATGA
SfoI 101 GGCGCC GGC^GCC
3161 GGCGCC
SfuI 1910 TTCGAA TT^CGAA
6813 TTCGAA
SpeI 4395 ACTAGT A^CTAGT
4745 ACTAGT
Tth111I 4205 GACTTTGTC GAC[ACGT]^[ACGT][ACGT]GTC
7859 GACTTAGTC
#########################################
# 3 OR MORE CUT ENZYMES ( 165 enzymes) #
#########################################
Acc65I AccII AccIII AciI AcsI
AcyI AfaI AflIII AluI AlwI
AlwNI AocI ApaI ApoI Asp700I
Asp718I AspHI AvaI AvaII BalI
BamHI BanI BanII BbsI BbvCI
BbvI BciVI BclI BcnI BfaI
BglI BglII BmyI BpmI BpuAI
BsaAI BsaHI BsaI BsaJI BsaWI
BseAI BseRI BsgI BsiHKAI BsiYI
BslI BsmAI BsmFI BsmI BsoBI
Bsp1286I Bsp1407I BspEI BspLU11I BspMI
BsrBI BsrFI BsrGI BsrI BssKI
BstAPI BstF5I BstNI BstUI BstXI
BstYI Bsu36I Cac8I CfoI Cfr10I
Cfr13I DdeI DpnII DraI DraII
DraIII DsaI EaeI EarI Eco81I
EcoNI EcoO109I EcoRI EcoRII EcoT14I
EcoT22I FauI FbaI Fnu4HI FokI
HaeII HaeIII HapII HgaI HhaI
Hin1I HinP1I HincII HindII HindIII
HinfI HpaII HphI ItaI KpnI
Ksp632I MaeI MaeII MaeIII MboI
MboII MflI MluNI MnlI MroI
MscI MseI MslI MspA1I MspI
MvaI MvnI MwoI NciI NcoI
NdeII NlaIII NlaIV NsiI NspI
PflMI PleI PpuMI PshAI PstI
PvuII RsaI SacI SapI Sau3AI
Sau96I ScaI ScrFI SexAI SfaNI
SfcI SmlI SphI SspBI SspI
StuI StyI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI TthHB8I
Van91I XbaI XcmI XhoII XmnI