##################################
# NO CUT ENZYMES (   79 enzymes) #
##################################

AatII       AccII       AccIII      AcyI        AgeI        
Alw44I      AlwNI       AocI        Aor51HI     ApaI        
ApaLI       AscI        AviII       BbeI        BbrPI       
BcgI        BlpI        Bpu1102I    BsaAI       BsaHI       
BsaI        BsaWI       BseAI       BsiEI       BsiWI       
BspDI       BspEI       BsrBI       BsrFI       BssHII      
BssSI       BstUI       Bsu36I      CelII       Cfr10I      
ClaI        CpoI        EagI        EclXI       Eco47III    
Eco52I      Eco81I      FseI        FspI        HaeII       
Hin1I       HpaI        KasI        KspI        MluI        
MroI        MvnI        NaeI        NarI        NgoMIV      
NotI        NruI        PacI        PaeR7I      PinAI       
PmaCI       PmeI        PmlI        PshAI       PvuI        
RsrII       SacI        SacII       SalI        SfiI        
SfoI        SgrAI       SmaI        SnaBI       SphI        
StuI        XhoI        XmaCI       XmaI        


###################################
# ONE CUT ENZYMES (   41 enzymes) #
###################################

Acc65I       3444  GGTACC               G^GTACC
AclI         1411  AACGTT               AA^CGTT
AhdI          338  GACCTAGAGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Asp718I      3444  GGTACC               G^GTACC
AspEI         338  GACCTAGAGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspHI        1768  GAGCAC               G[AT]GC[AT]^C
AvaI          236  CCCGAG               C^[CT]CG[GA]G
BanII        1174  GAGCCC               G[GA]GC[CT]^C
BbvCI         813  CCTCAGC              CCTCAGC(-5/-2)
BciVI        3282  GTATCC               GTATCC(6/5)
BglI         1111  GCCTGCTGGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsiHKAI      1768  GAGCAC               G[AT]GC[AT]^C
BsmBI        4763  GAGACG               CGTCTC(1/5)
BsoBI         236  CCCGAG               C^[CT]CG[GA]G
Bsp1407I     3195  TGTACA               T^GTACA
BspMI        5605  GCAGGT               ACCTGC(4/8)
BsrGI        3195  TGTACA               T^GTACA
Bst1107I     5048  GTATAC               GTA^TAC
BstBI         759  TTCGAA               TT^CGAA
BstZ17I      5048  GTATAC               GTA^TAC
DrdI         2106  GACAGATTTGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eam1105I      338  GACCTAGAGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoRI        1892  GAATTC               G^AATTC
HgaI         5771  GCGTC                GACGC(5/10)
HincII       3945  GTTGAC               GT[CT]^[GA]AC
HindII       3945  GTTGAC               GT[CT]^[GA]AC
KpnI         3444  GGTACC               GGTAC^C
NheI         2244  GCTAGC               G^CTAGC
NspV          759  TTCGAA               TT^CGAA
PflMI        3432  CCAGGCTGTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Psp1406I     1411  AACGTT               AA^CGTT
SapI          278  GAAGAGC              GCTCTTC(1/4)
SbfI         1343  CCTGCAGG             CCTGCA^GG
ScaI         5689  AGTACT               AGT^ACT
SexAI         539  ACCAGGT              A^CC[AT]GGT
SfuI          759  TTCGAA               TT^CGAA
SpeI         4056  ACTAGT               A^CTAGT
Sse8387I     1343  CCTGCAGG             CCTGCA^GG
SspBI        3195  TGTACA               T^GTACA
SwaI         6322  ATTTAAAT             ATTT^AAAT
Van91I       3432  CCAGGCTGTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG


###################################
# TWO CUT ENZYMES (   26 enzymes) #
###################################

AflII         324  CTTAAG               C^TTAAG
             1284  CTTAAG              
AflIII       2641  ACATGT               A^C[GA][CT]GT
             4774  ACATGT              
AseI         1290  ATTAAT               AT^TAAT
             4376  ATTAAT              
AsnI         1290  ATTAAT               AT^TAAT
             4376  ATTAAT              
AvrII         711  CCTAGG               C^CTAGG
             5909  CCTAGG              
BfrI          324  CTTAAG               C^TTAAG
             1284  CTTAAG              
BglII        2978  AGATCT               A^GATCT
             4991  AGATCT              
BlnI          711  CCTAGG               C^CTAGG
             5909  CCTAGG              
BseRI         358  CTCCTC               GAGGAG(10/8)
             5178  GAGGAG              
BspLU11I     2641  ACATGT               A^CATGT
             4774  ACATGT              
BstAPI       1307  GCAAATTCTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
             3528  GCAGAAATTGC         
BstEII       3557  GGTCACC              G^GT[ACGT]ACC
             4744  GGTCACC             
BstPI        3557  GGTCACC              G^GT[ACGT]ACC
             4744  GGTCACC             
BstXI        1828  CCACGGAAGTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             2745  CCACATTTATGG        
CfoI         4334  GCGC                 GCG^C
             5597  GCGC                
DraIII       4024  CACAGTGTG            CAC[ACGT][ACGT][ACGT]^GTG
             6145  CACAAAGTG           
EcoO65I      3557  GGTCACC              G^GT[ACGT]ACC
             4744  GGTCACC             
FauI         3111  CCCGC                CCCGC(4/6)
             6131  CCCGC               
HhaI         4334  GCGC                 GCG^C
             5597  GCGC                
HinP1I       4334  GCGC                 G^CGC
             5597  GCGC                
MfeI         1683  CAATTG               C^AATTG
             6272  CAATTG              
MspA1I       1124  CAGCTG               C[AC]G^C[GT]G
             2684  CAGCTG              
MunI         1683  CAATTG               C^AATTG
             6272  CAATTG              
NdeI         4884  CATATG               CA^TATG
             5938  CATATG              
PshBI        1290  ATTAAT               AT^TAAT
             4376  ATTAAT              
PvuII        1124  CAGCTG               CAG^CTG
             2684  CAGCTG              


#########################################
# 3 OR MORE CUT ENZYMES (  112 enzymes) #
#########################################

AccI        AciI        AcsI        AfaI        AluI        
AlwI        ApoI        Asp700I     AspI        AvaII       
BalI        BamHI       BanI        BbsI        BbvI        
BclI        BcnI        BfaI        BmyI        BpmI        
BpuAI       BsaBI       BsaJI       BsgI        BsiYI       
BslI        BsmAI       BsmFI       BsmI        Bsp1286I    
BspHI       BsrDI       BsrI        BssKI       BstF5I      
BstNI       BstYI       Cac8I       Cfr13I      DdeI        
DpnII       DraI        DraII       DsaI        EaeI        
EarI        EcoNI       EcoO109I    EcoRII      EcoRV       
EcoT14I     EcoT22I     FbaI        Fnu4HI      FokI        
HaeIII      HapII       HindIII     HinfI       HpaII       
HphI        ItaI        Ksp632I     MaeI        MaeII       
MaeIII      MamI        MboI        MboII       MflI        
MluNI       MnlI        MscI        MseI        MslI        
MspI        MvaI        MwoI        NciI        NcoI        
NdeII       NlaIII      NlaIV       NsiI        NspI        
PflFI       PleI        PpuMI       PstI        RcaI        
RsaI        Sau3AI      Sau96I      ScrFI       SfaNI       
SfcI        SmlI        SspI        StyI        TaqI        
TfiI        Tru9I       TseI        Tsp45I      Tsp509I     
TspRI       Tth111I     TthHB8I     XbaI        XcmI        
XhoII       XmnI