##################################
# NO CUT ENZYMES (   96 enzymes) #
##################################

AatII       Acc65I      AclI        AcyI        AflII       
AgeI        AhdI        AlwNI       AocI        Aor51HI     
ApaI        AscI        Asp718I     AspEI       AvaI        
AviII       AvrII       BbeI        BbrPI       BbvCI       
BcgI        BcnI        BfrI        BglI        BlnI        
BlpI        Bpu1102I    BsaHI       BsiEI       BsiWI       
BsoBI       BspDI       BsrBI       BssHII      BssSI       
Bst1107I    BstBI       BstZ17I     Bsu36I      CelII       
CfoI        ClaI        CpoI        DraII       EagI        
Eam1105I    EclXI       Eco47III    Eco52I      Eco81I      
EcoO109I    FseI        FspI        HaeII       HgaI        
HhaI        Hin1I       HinP1I      KasI        KpnI        
KspI        MluI        NaeI        NarI        NciI        
NgoMIV      NheI        NotI        NruI        NspV        
PacI        PaeR7I      PinAI       PmaCI       PmeI        
PmlI        PpuMI       Psp1406I    PvuI        RsrII       
SacII       SalI        SbfI        SfiI        SfoI        
SfuI        SgrAI       SmaI        SpeI        SphI        
Sse8387I    StuI        SwaI        XhoI        XmaCI       
XmaI        


###################################
# ONE CUT ENZYMES (   37 enzymes) #
###################################

AccII        3322  CGCG                 CG^CG
AccIII       2906  TCCGGA               T^CCGGA
AspI         5996  GACATGGTC            GAC[ACGT]^[ACGT][ACGT]GTC
BanI         3085  GGCACC               G^G[CT][GA]CC
BsaI         6035  GGTCTC               GGTCTC(1/5)
BsaWI        2906  TCCGGA               [AT]^CCGG[AT]
BseAI        2906  TCCGGA               T^CCGGA
BseRI        5394  GAGGAG               GAGGAG(10/8)
BsgI         2366  GTGCAG               GTGCAG(16/14)
BsmBI        1636  GAGACG               CGTCTC(1/5)
Bsp1407I     5091  TGTACA               T^GTACA
BspEI        2906  TCCGGA               T^CCGGA
BspMI        1691  GCAGGT               ACCTGC(4/8)
BsrFI        5051  ACCGGC               [GA]^CCGG[CT]
BsrGI        5091  TGTACA               T^GTACA
BstEII       2532  GGTAACC              G^GT[ACGT]ACC
BstPI        2532  GGTAACC              G^GT[ACGT]ACC
BstUI        3322  CGCG                 CG^CG
Cfr10I       5051  ACCGGC               [GA]^CCGG[CT]
DraIII       3011  CACAGTGTG            CAC[ACGT][ACGT][ACGT]^GTG
EcoNI         751  CCTGAAACAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I      2532  GGTAACC              G^GT[ACGT]ACC
EcoRV        2143  GATATC               GAT^ATC
FauI         4867  CCCGC                CCCGC(4/6)
HindIII      1562  AAGCTT               A^AGCTT
MroI         2906  TCCGGA               T^CCGGA
MspA1I       1671  CAGCTG               C[AC]G^C[GT]G
MvnI         3322  CGCG                 CG^CG
PflFI        5996  GACATGGTC            GAC[ACGT]^[ACGT][ACGT]GTC
PshAI        5239  GACTGGAGTC           GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PstI         2632  CTGCAG               CTGCA^G
PvuII        1671  CAGCTG               CAG^CTG
SacI          625  GAGCTC               GAGCT^C
SexAI        6145  ACCTGGT              A^CC[AT]GGT
SspBI        5091  TGTACA               T^GTACA
Tth111I      5996  GACATGGTC            GAC[ACGT]^[ACGT][ACGT]GTC
XcmI         6199  CCAAAAAAATGTTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG


###################################
# TWO CUT ENZYMES (   36 enzymes) #
###################################

AflIII        682  ACATGT               A^C[GA][CT]GT
             5120  ACATGT              
Alw44I       1936  GTGCAC               G^TGCAC
             5979  GTGCAC              
ApaLI        1936  GTGCAC               G^TGCAC
             5979  GTGCAC              
AseI         1983  ATTAAT               AT^TAAT
             4726  ATTAAT              
AsnI         1983  ATTAAT               AT^TAAT
             4726  ATTAAT              
BalI         4373  TGGCCA               TGG^CCA
             4522  TGGCCA              
BamHI        2741  GGATCC               G^GATCC
             3924  GGATCC              
BanII         625  GAGCTC               G[GA]GC[CT]^C
             4240  GGGCTC              
BclI         2861  TGATCA               T^GATCA
             6029  TGATCA              
BsaBI          95  GATTTTGATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
              256  GATGTAAATC          
BspHI        2147  TCATGA               T^CATGA
             2864  TCATGA              
BspLU11I      682  ACATGT               A^CATGT
             5120  ACATGT              
BstAPI       3288  GCAAGATATGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
             3670  GCAGAACATGC         
BstXI        1732  CCAAGTTCCTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             5728  CCAATGTACTGG        
Cac8I        5269  GCTGGC               GC[ACGT]^[ACGT]GC
             6535  GCAAGC              
DsaI          933  CCATGG               C^C[GA][CT]GG
             6372  CCATGG              
EaeI         4373  TGGCCA               [CT]^GGCC[GA]
             4522  TGGCCA              
EcoT22I      3446  ATGCAT               ATGCA^T
             4615  ATGCAT              
FbaI         2861  TGATCA               T^GATCA
             6029  TGATCA              
HapII        2907  CCGG                 C^CGG
             5052  CCGG                
HpaI         5203  GTTAAC               GTT^AAC
             6522  GTTAAC              
HpaII        2907  CCGG                 C^CGG
             5052  CCGG                
MamI           95  GATTTTGATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
              256  GATGTAAATC          
MfeI         4634  CAATTG               C^AATTG
             4812  CAATTG              
MluNI        4373  TGGCCA               TGG^CCA
             4522  TGGCCA              
MscI         4373  TGGCCA               TGG^CCA
             4522  TGGCCA              
MspI         2907  CCGG                 C^CGG
             5052  CCGG                
MunI         4634  CAATTG               C^AATTG
             4812  CAATTG              
NcoI          933  CCATGG               C^CATGG
             6372  CCATGG              
NdeI         2981  CATATG               CA^TATG
             4612  CATATG              
NsiI         3446  ATGCAT               ATGCA^T
             4615  ATGCAT              
PshBI        1983  ATTAAT               AT^TAAT
             4726  ATTAAT              
RcaI         2147  TCATGA               T^CATGA
             2864  TCATGA              
SapI         2968  GCTCTTC              GCTCTTC(1/4)
             5079  GCTCTTC             
SnaBI        2575  TACGTA               TAC^GTA
             5784  TACGTA              
XbaI          182  TCTAGA               T^CTAGA
             6446  TCTAGA              


#########################################
# 3 OR MORE CUT ENZYMES (   89 enzymes) #
#########################################

AccI        AciI        AcsI        AfaI        AluI        
AlwI        ApoI        Asp700I     AspHI       AvaII       
BbsI        BbvI        BciVI       BfaI        BglII       
BmyI        BpmI        BpuAI       BsaAI       BsaJI       
BsiHKAI     BsiYI       BslI        BsmAI       BsmFI       
BsmI        Bsp1286I    BsrDI       BsrI        BssKI       
BstF5I      BstNI       BstYI       Cfr13I      DdeI        
DpnII       DraI        DrdI        EarI        EcoRI       
EcoRII      EcoT14I     Fnu4HI      FokI        HaeIII      
HincII      HindII      HinfI       HphI        ItaI        
Ksp632I     MaeI        MaeII       MaeIII      MboI        
MboII       MflI        MnlI        MseI        MslI        
MvaI        MwoI        NdeII       NlaIII      NlaIV       
NspI        PflMI       PleI        RsaI        Sau3AI      
Sau96I      ScaI        ScrFI       SfaNI       SfcI        
SmlI        SspI        StyI        TaqI        TfiI        
Tru9I       TseI        Tsp45I      Tsp509I     TspRI       
TthHB8I     Van91I      XhoII       XmnI