##################################
# NO CUT ENZYMES ( 92 enzymes) #
##################################
AatII Acc65I AccII AcyI AgeI
AhdI Aor51HI ApaI AscI Asp718I
AspEI AvaI AviII AvrII BalI
BamHI BbeI BbrPI BbvCI BglI
BlnI BsaHI BsaI BsiWI BsoBI
BspDI BspMI BsrFI BssHII BssSI
Bst1107I BstBI BstUI BstXI BstZ17I
Cfr10I ClaI CpoI DraII DraIII
DrdI EaeI EagI Eam1105I EclXI
Eco47III Eco52I EcoNI EcoO109I EcoRV
FseI FspI HaeII Hin1I KasI
KpnI KspI MfeI MluI MluNI
MscI MunI MvnI NaeI NarI
NgoMIV NotI NruI NspV PaeR7I
PinAI PmaCI PmeI PmlI PpuMI
PshAI RsrII SacII SbfI SfiI
SfoI SfuI SgrAI SmaI SnaBI
SpeI Sse8387I SwaI XcmI XhoI
XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 49 enzymes) #
###################################
AccIII 4921 TCCGGA T^CCGGA
AclI 3267 AACGTT AA^CGTT
AflII 386 CTTAAG C^TTAAG
Alw44I 2538 GTGCAC G^TGCAC
AocI 3779 CCTCAGG CC^T[ACGT]AGG
ApaLI 2538 GTGCAC G^TGCAC
BanI 3860 GGTGCC G^G[CT][GA]CC
BcgI 2854 GCATTTTGTTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 2902 GGATAC GTATCC(6/5)
BcnI 3320 CCGGG CC^[GC]GG
BfrI 386 CTTAAG C^TTAAG
BlpI 1370 GCTAAGC GC^T[ACGT]AGC
Bpu1102I 1370 GCTAAGC GC^T[ACGT]AGC
BsaAI 2964 TACGTG [CT]AC^GT[GA]
BseAI 4921 TCCGGA T^CCGGA
BsgI 1392 CTGCAC GTGCAG(16/14)
BsiEI 4330 CGATCG CG[GA][CT]^CG
BsmBI 2155 GAGACG CGTCTC(1/5)
BspEI 4921 TCCGGA T^CCGGA
BspHI 501 TCATGA T^CATGA
BsrBI 153 GAGCGG CCGCTC(-3/-3)
BsrDI 3450 GCAATG GCAATG(2/0)
BstEII 4975 GGTTACC G^GT[ACGT]ACC
BstPI 4975 GGTTACC G^GT[ACGT]ACC
Bsu36I 3779 CCTCAGG CC^T[ACGT]AGG
CelII 1370 GCTAAGC GC^T[ACGT]AGC
CfoI 4156 GCGC GCG^C
Eco81I 3779 CCTCAGG CC^T[ACGT]AGG
EcoO65I 4975 GGTTACC G^GT[ACGT]ACC
FauI 3447 CCCGC CCCGC(4/6)
HgaI 99 GACGC GACGC(5/10)
HhaI 4156 GCGC GCG^C
HinP1I 4156 GCGC G^CGC
HpaI 2303 GTTAAC GTT^AAC
MroI 4921 TCCGGA T^CCGGA
MslI 143 CACATGCATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NciI 3320 CCGGG CC^[GC]GG
NheI 2431 GCTAGC G^CTAGC
PacI 1658 TTAATTAA TTAAT^TAA
PflMI 1435 CCATGTCTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Psp1406I 3267 AACGTT AA^CGTT
PvuI 4330 CGATCG CGAT^CG
RcaI 501 TCATGA T^CATGA
SalI 4752 GTCGAC G^TCGAC
ScaI 1382 AGTACT AGT^ACT
SexAI 3387 ACCAGGT A^CC[AT]GGT
StuI 1903 AGGCCT AGG^CCT
Van91I 1435 CCATGTCTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XbaI 2455 TCTAGA T^CTAGA
###################################
# TWO CUT ENZYMES ( 16 enzymes) #
###################################
AccI 4752 GTCGAC GT^[AC][GT]AC
5135 GTCTAC
AlwNI 930 CAGATGCTG CAG[ACGT][ACGT][ACGT]^CTG
1593 CAGAAGCTG
AspI 813 GACAAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
1822 GACCAAGTC
BglII 2161 AGATCT A^GATCT
3817 AGATCT
BsiYI 1435 CCATGTCTTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
4994 CCATGTGGGGG
BslI 1435 CCATGTCTTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
4994 CCATGTGGGGG
BsmFI 3229 GGGAC GGGAC(10/14)
3244 GTCCC
EcoT14I 3201 CCATGG C^C[AT][AT]GG
3396 CCATGG
HindIII 396 AAGCTT A^AGCTT
5110 AAGCTT
NcoI 3201 CCATGG C^CATGG
3396 CCATGG
NlaIV 3860 GGTGCC GG[ACGT]^[ACGT]CC
4217 GGTTCC
PflFI 813 GACAAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
1822 GACCAAGTC
SapI 4619 GCTCTTC GCTCTTC(1/4)
4679 GCTCTTC
SphI 1155 GCATGC GCATG^C
2868 GCATGC
StyI 3201 CCATGG C^C[AT][AT]GG
3396 CCATGG
Tth111I 813 GACAAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
1822 GACCAAGTC
#########################################
# 3 OR MORE CUT ENZYMES ( 101 enzymes) #
#########################################
AciI AcsI AfaI AflIII AluI
AlwI ApoI AseI AsnI Asp700I
AspHI AvaII BanII BbsI BbvI
BclI BfaI BmyI BpmI BpuAI
BsaBI BsaJI BsaWI BseRI BsiHKAI
BsmAI BsmI Bsp1286I Bsp1407I BspLU11I
BsrGI BsrI BssKI BstAPI BstF5I
BstNI BstYI Cac8I Cfr13I DdeI
DpnII DraI DsaI EarI EcoRI
EcoRII EcoT22I FbaI Fnu4HI FokI
HaeIII HapII HincII HindII HinfI
HpaII HphI ItaI Ksp632I MaeI
MaeII MaeIII MamI MboI MboII
MflI MnlI MseI MspA1I MspI
MvaI MwoI NdeI NdeII NlaIII
NsiI NspI PleI PshBI PstI
PvuII RsaI SacI Sau3AI Sau96I
ScrFI SfaNI SfcI SmlI SspBI
SspI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I XhoII
XmnI