##################################
# NO CUT ENZYMES (   75 enzymes) #
##################################

AatII       AccII       AclI        AcyI        AflII       
AgeI        AscI        AseI        AsnI        BbeI        
BbrPI       BclI        BfrI        BsaAI       BsaHI       
BsiEI       BsiWI       BsmBI       Bsp1407I    BspDI       
BsrFI       BsrGI       BssHII      BstUI       Cfr10I      
ClaI        CpoI        DrdI        EagI        EclXI       
Eco52I      FauI        FbaI        FseI        Hin1I       
KasI        KspI        MfeI        MluI        MunI        
MvnI        NaeI        NarI        NdeI        NgoMIV      
NotI        NruI        PacI        PflMI       PinAI       
PmaCI       PmeI        PmlI        PshAI       PshBI       
Psp1406I    PvuI        RsrII       SacII       SalI        
SbfI        SfiI        SfoI        SgrAI       SmaI        
SnaBI       SpeI        Sse8387I    SspBI       SspI        
StuI        SwaI        Van91I      XmaCI       XmaI        


###################################
# ONE CUT ENZYMES (   56 enzymes) #
###################################

AflIII       1839  ACATGT               A^C[GA][CT]GT
Alw44I       2648  GTGCAC               G^TGCAC
AocI         4079  CCTTAGG              CC^T[ACGT]AGG
ApaLI        2648  GTGCAC               G^TGCAC
Asp700I       190  GAAATTTTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspI         4170  GACCCTGTC            GAC[ACGT]^[ACGT][ACGT]GTC
AviII         903  TGCGCA               TGC^GCA
AvrII        2514  CCTAGG               C^CTAGG
BamHI        1115  GGATCC               G^GATCC
BcgI          463  GCAGAAAAGTCG         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BcnI            7  CCGGG                CC^[GC]GG
BglI          363  GCCCCAGTGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII        3351  AGATCT               A^GATCT
BlnI         2514  CCTAGG               C^CTAGG
BlpI         3029  GCTGAGC              GC^T[ACGT]AGC
Bpu1102I     3029  GCTGAGC              GC^T[ACGT]AGC
BsaBI        1813  GATGCACATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsmI         1337  GCATTC               GAATGC(1/-1)
BspLU11I     1839  ACATGT               A^CATGT
BsrBI        1008  CCGCTC               CCGCTC(-3/-3)
BssSI        2676  CTCGTG               CACGAG(-5/-1)
BstAPI        859  GCAGTGGGTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstBI        2365  TTCGAA               TT^CGAA
BstEII       2186  GGTGACC              G^GT[ACGT]ACC
BstPI        2186  GGTGACC              G^GT[ACGT]ACC
Bsu36I       4079  CCTTAGG              CC^T[ACGT]AGG
CelII        3029  GCTGAGC              GC^T[ACGT]AGC
DraIII       2569  CACACGGTG            CAC[ACGT][ACGT][ACGT]^GTG
DsaI         3953  CCATGG               C^C[GA][CT]GG
Eco81I       4079  CCTTAGG              CC^T[ACGT]AGG
EcoO65I      2186  GGTGACC              G^GT[ACGT]ACC
EcoRI        3931  GAATTC               G^AATTC
EcoRV         934  GATATC               GAT^ATC
EcoT22I      4049  ATGCAT               ATGCA^T
FspI          903  TGCGCA               TGC^GCA
HgaI          712  GACGC                GACGC(5/10)
HincII       3129  GTTAAC               GT[CT]^[GA]AC
HindII       3129  GTTAAC               GT[CT]^[GA]AC
HpaI         3129  GTTAAC               GTT^AAC
MamI         1813  GATGCACATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
NciI            7  CCGGG                CC^[GC]GG
NcoI         3953  CCATGG               C^CATGG
NheI         1528  GCTAGC               G^CTAGC
NsiI         4049  ATGCAT               ATGCA^T
NspV         2365  TTCGAA               TT^CGAA
PaeR7I       2904  CTCGAG               C^TCGAG
PflFI        4170  GACCCTGTC            GAC[ACGT]^[ACGT][ACGT]GTC
SapI          721  GAAGAGC              GCTCTTC(1/4)
ScaI         4116  AGTACT               AGT^ACT
SexAI        2259  ACCTGGT              A^CC[AT]GGT
SfuI         2365  TTCGAA               TT^CGAA
SphI         1456  GCATGC               GCATG^C
Tth111I      4170  GACCCTGTC            GAC[ACGT]^[ACGT][ACGT]GTC
XbaI         3004  TCTAGA               T^CTAGA
XhoI         2904  CTCGAG               C^TCGAG
XmnI          190  GAAATTTTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   27 enzymes) #
###################################

Acc65I        503  GGTACC               G^GTACC
             1541  GGTACC              
AccIII       2197  TCCGGA               T^CCGGA
             3480  TCCGGA              
Aor51HI       563  AGCGCT               AGC^GCT
              928  AGCGCT              
Asp718I       503  GGTACC               G^GTACC
             1541  GGTACC              
BalI         2527  TGGCCA               TGG^CCA
             2890  TGGCCA              
BbsI          776  GAAGAC               GAAGAC(2/6)
              812  GTCTTC              
BpuAI         776  GAAGAC               GAAGAC(2/6)
              812  GTCTTC              
BsaI         1807  GAGACC               GGTCTC(1/5)
             4168  GAGACC              
BsaWI        2197  TCCGGA               [AT]^CCGG[AT]
             3480  TCCGGA              
BseAI        2197  TCCGGA               T^CCGGA
             3480  TCCGGA              
BspEI        2197  TCCGGA               T^CCGGA
             3480  TCCGGA              
BsrDI         511  CATTGC               GCAATG(2/0)
              757  CATTGC              
Bst1107I     1711  GTATAC               GTA^TAC
             2094  GTATAC              
BstXI        2806  CCAAAGCATTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             3887  CCAGCACTTTGG        
BstZ17I      1711  GTATAC               GTA^TAC
             2094  GTATAC              
EaeI         2527  TGGCCA               [CT]^GGCC[GA]
             2890  TGGCCA              
Eco47III      563  AGCGCT               AGC^GCT
              928  AGCGCT              
EcoNI        1469  CCTCAGTCAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
             3659  CCTTTTTGAGG         
KpnI          503  GGTACC               GGTAC^C
             1541  GGTACC              
MluNI        2527  TGGCCA               TGG^CCA
             2890  TGGCCA              
MroI         2197  TCCGGA               T^CCGGA
             3480  TCCGGA              
MscI         2527  TGGCCA               TGG^CCA
             2890  TGGCCA              
NspI         1456  GCATGC               [GA]CATG^[CT]
             1839  ACATGT              
PpuMI         337  AGGACCT              [GA]G^G[AT]CC[CT]
             1198  GGGTCCT             
PstI         2335  CTGCAG               CTGCA^G
             3362  CTGCAG              
SacI         1410  GAGCTC               GAGCT^C
             3032  GAGCTC              
SmlI         2342  CTTGAG               C^T[CT][GA]AG
             2904  CTCGAG              


#########################################
# 3 OR MORE CUT ENZYMES (  100 enzymes) #
#########################################

AccI        AciI        AcsI        AfaI        AhdI        
AluI        AlwI        AlwNI       ApaI        ApoI        
AspEI       AspHI       AvaI        AvaII       BanI        
BanII       BbvCI       BbvI        BciVI       BfaI        
BmyI        BpmI        BsaJI       BseRI       BsgI        
BsiHKAI     BsiYI       BslI        BsmAI       BsmFI       
BsoBI       Bsp1286I    BspHI       BspMI       BsrI        
BssKI       BstF5I      BstNI       BstYI       Cac8I       
CfoI        Cfr13I      DdeI        DpnII       DraI        
DraII       Eam1105I    EarI        EcoO109I    EcoRII      
EcoT14I     Fnu4HI      FokI        HaeII       HaeIII      
HapII       HhaI        HinP1I      HindIII     HinfI       
HpaII       HphI        ItaI        Ksp632I     MaeI        
MaeII       MaeIII      MboI        MboII       MflI        
MnlI        MseI        MslI        MspA1I      MspI        
MvaI        MwoI        NdeII       NlaIII      NlaIV       
PleI        PvuII       RcaI        RsaI        Sau3AI      
Sau96I      ScrFI       SfaNI       SfcI        StyI        
TaqI        TfiI        Tru9I       TseI        Tsp45I      
Tsp509I     TspRI       TthHB8I     XcmI        XhoII