################################## # NO CUT ENZYMES ( 73 enzymes) # ################################## Acc65I AccIII AclI AflII AgeI AhdI AocI Aor51HI AscI AseI AsnI Asp700I Asp718I AspEI AspI AvrII BfrI BglII BlnI BseAI BsiWI Bsp1407I BspDI BspEI BspLU11I BsrGI BstBI BstEII BstPI Bsu36I ClaI CpoI Eam1105I Eco47III Eco81I EcoO65I EcoRV EcoT22I FseI HpaI KpnI MfeI MluI MroI MunI NdeI NotI NruI NsiI NspV PacI PaeR7I PflFI PflMI PinAI PmeI PshBI Psp1406I PvuI RsrII SalI SapI ScaI SfuI SgrAI SnaBI SpeI SspBI SwaI Tth111I Van91I XhoI XmnI ################################### # ONE CUT ENZYMES ( 36 enzymes) # ################################### AatII 2353 GACGTC GACGT^C AccI 1352 GTATAC GT^[AC][GT]AC AflIII 3936 ACACGT A^C[GA][CT]GT Alw44I 3460 GTGCAC G^TGCAC ApaLI 3460 GTGCAC G^TGCAC BamHI 73 GGATCC G^GATCC BbrPI 4123 CACGTG CAC^GTG BbvCI 2190 GCTGAGG CCTCAGC(-5/-2) BsaWI 140 TCCGGT [AT]^CCGG[AT] BsmI 383 GAATGC GAATGC(1/-1) BspHI 3441 TCATGA T^CATGA Bst1107I 1352 GTATAC GTA^TAC BstAPI 2902 GCAGGAGGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstZ17I 1352 GTATAC GTA^TAC DraIII 596 CACCTGGTG CAC[ACGT][ACGT][ACGT]^GTG DrdI 4058 GACTCCTCTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EagI 2335 CGGCCG C^GGCCG EarI 1582 GAAGAG CTCTTC(1/4) EclXI 2335 CGGCCG C^GGCCG Eco52I 2335 CGGCCG C^GGCCG HindIII 3238 AAGCTT A^AGCTT Ksp632I 1582 GAAGAG CTCTTC(1/4) NheI 353 GCTAGC G^CTAGC PmaCI 4123 CACGTG CAC^GTG PmlI 4123 CACGTG CAC^GTG PshAI 1934 GACCGGGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC RcaI 3441 TCATGA T^CATGA SbfI 2899 CCTGCAGG CCTGCA^GG SexAI 597 ACCTGGT A^CC[AT]GGT SfiI 2160 GGCCGCGGGGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC SmlI 1239 CTCAAG C^T[CT][GA]AG Sse8387I 2899 CCTGCAGG CCTGCA^GG SspI 2985 AATATT AAT^ATT StuI 477 AGGCCT AGG^CCT TfiI 1435 GATTC G^A[AT]TC XcmI 1451 CCAACTCAGGACTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG ################################### # TWO CUT ENZYMES ( 20 enzymes) # ################################### BalI 293 TGGCCA TGG^CCA 3755 TGGCCA BcgI 334 GCAGGGATCTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) 3562 GCAGACCACTCG BciVI 362 GGATAC GTATCC(6/5) 815 GGATAC BlpI 432 GCTCAGC GC^T[ACGT]AGC 2324 GCTGAGC Bpu1102I 432 GCTCAGC GC^T[ACGT]AGC 2324 GCTGAGC BsaAI 1770 CACGTA [CT]AC^GT[GA] 4123 CACGTG BsaBI 739 GATCAAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC 1647 GATGGGCATC BsmBI 3775 CGTCTC CGTCTC(1/5) 3038 GAGACG BsrDI 3265 CATTGC GCAATG(2/0) 3980 CATTGC CelII 432 GCTCAGC GC^T[ACGT]AGC 2324 GCTGAGC EcoNI 198 CCTGCAGCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG 2899 CCTGCAGGAGG EcoRI 3445 GAATTC G^AATTC 3739 GAATTC HincII 854 GTCAAC GT[CT]^[GA]AC 2957 GTTGAC HindII 854 GTCAAC GT[CT]^[GA]AC 2957 GTTGAC MamI 739 GATCAAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC 1647 GATGGGCATC MluNI 293 TGGCCA TGG^CCA 3755 TGGCCA MscI 293 TGGCCA TGG^CCA 3755 TGGCCA SacI 2327 GAGCTC GAGCT^C 4073 GAGCTC SphI 630 GCATGC GCATG^C 2033 GCATGC XbaI 3046 TCTAGA T^CTAGA 3855 TCTAGA ######################################### # 3 OR MORE CUT ENZYMES ( 129 enzymes) # ######################################### AccII AciI AcsI AcyI AfaI AluI AlwI AlwNI ApaI ApoI AspHI AvaI AvaII AviII BanI BanII BbeI BbsI BbvI BclI BcnI BfaI BglI BmyI BpmI BpuAI BsaHI BsaI BsaJI BseRI BsgI BsiEI BsiHKAI BsiYI BslI BsmAI BsmFI BsoBI Bsp1286I BspMI BsrBI BsrFI BsrI BssHII BssKI BssSI BstF5I BstNI BstUI BstXI BstYI Cac8I CfoI Cfr10I Cfr13I DdeI DpnII DraI DraII DsaI EaeI EcoO109I EcoRII EcoT14I FauI FbaI Fnu4HI FokI FspI HaeII HaeIII HapII HgaI HhaI Hin1I HinP1I HinfI HpaII HphI ItaI KasI KspI MaeI MaeII MaeIII MboI MboII MflI MnlI MseI MslI MspA1I MspI MvaI MvnI MwoI NaeI NarI NciI NcoI NdeII NgoMIV NlaIII NlaIV NspI PleI PpuMI PstI PvuII RsaI SacII Sau3AI Sau96I ScrFI SfaNI SfcI SfoI SmaI StyI TaqI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I XhoII XmaCI XmaI