################################## # NO CUT ENZYMES ( 54 enzymes) # ################################## AatII AccIII AclI AflIII AgeI AhdI Aor51HI AscI AseI AsnI AspEI BsaBI BseAI BsiWI BspDI BspEI BspLU11I BssHII Bst1107I BstBI BstZ17I ClaI CpoI DraI DrdI Eam1105I Eco47III EcoRV EcoT22I HindIII HpaI MamI MfeI MluI MroI MunI NdeI NruI NsiI NspV PacI PinAI PmeI PshAI PshBI Psp1406I PvuI RsrII SalI ScaI SfuI SnaBI SspI SwaI ################################### # ONE CUT ENZYMES ( 42 enzymes) # ################################### Acc65I 2516 GGTACC G^GTACC AccI 3691 GTCTAC GT^[AC][GT]AC AflII 6380 CTTAAG C^TTAAG Alw44I 6141 GTGCAC G^TGCAC ApaLI 6141 GTGCAC G^TGCAC Asp718I 2516 GGTACC G^GTACC AspI 6372 GACTTTGTC GAC[ACGT]^[ACGT][ACGT]GTC AviII 5115 TGCGCA TGC^GCA AvrII 3443 CCTAGG C^CTAGG BamHI 2107 GGATCC G^GATCC BbeI 3516 GGCGCC GGCGC^C BciVI 2470 GGATAC GTATCC(6/5) BclI 1217 TGATCA T^GATCA BfrI 6380 CTTAAG C^TTAAG BlnI 3443 CCTAGG C^CTAGG BsaI 4476 GAGACC GGTCTC(1/5) BsaWI 1898 TCCGGT [AT]^CCGG[AT] BsmI 6387 GCATTC GAATGC(1/-1) BsrDI 6261 GCAATG GCAATG(2/0) BstEII 1035 GGTCACC G^GT[ACGT]ACC BstPI 1035 GGTCACC G^GT[ACGT]ACC EcoO65I 1035 GGTCACC G^GT[ACGT]ACC EcoRI 1990 GAATTC G^AATTC FbaI 1217 TGATCA T^GATCA FseI 5486 GGCCGGCC GGCCGG^CC FspI 5115 TGCGCA TGC^GCA HincII 1662 GTTGAC GT[CT]^[GA]AC HindII 1662 GTTGAC GT[CT]^[GA]AC KasI 3516 GGCGCC G^GCGCC KpnI 2516 GGTACC GGTAC^C NarI 3516 GGCGCC GG^CGCC NheI 2556 GCTAGC G^CTAGC NotI 1 GCGGCCGC GC^GGCCGC PaeR7I 3673 CTCGAG C^TCGAG PflFI 6372 GACTTTGTC GAC[ACGT]^[ACGT][ACGT]GTC SfiI 5486 GGCCGGCCAGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC SfoI 3516 GGCGCC GGC^GCC SgrAI 3653 CGCCGGTG C[GA]^CCGG[CT]G StuI 4024 AGGCCT AGG^CCT Tth111I 6372 GACTTTGTC GAC[ACGT]^[ACGT][ACGT]GTC XbaI 2075 TCTAGA T^CTAGA XhoI 3673 CTCGAG C^TCGAG ################################### # TWO CUT ENZYMES ( 29 enzymes) # ################################### AcsI 655 AAATTC [GA]^AATT[CT] 1990 GAATTC AcyI 3192 GGCGTC G[GA]^CG[CT]C 3516 GGCGCC ApoI 655 AAATTC [GA]^AATT[CT] 1990 GAATTC BbrPI 3009 CACGTG CAC^GTG 6014 CACGTG BcgI 5127 CGACAGTCCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) 3665 GCACCTCCCTCG BglII 2833 AGATCT A^GATCT 2842 AGATCT BlpI 1434 GCTCAGC GC^T[ACGT]AGC 4921 GCTGAGC Bpu1102I 1434 GCTCAGC GC^T[ACGT]AGC 4921 GCTGAGC BsaAI 3009 CACGTG [CT]AC^GT[GA] 6014 CACGTG BsaHI 3192 GGCGTC G[GA]^CG[CT]C 3516 GGCGCC BspHI 1220 TCATGA T^CATGA 1698 TCATGA BsrBI 83 CCGCTC CCGCTC(-3/-3) 776 GAGCGG BstAPI 1596 GCAGCCCTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC 1647 GCATCCTGTGC CelII 1434 GCTCAGC GC^T[ACGT]AGC 4921 GCTGAGC DraIII 1386 CACACGGTG CAC[ACGT][ACGT][ACGT]^GTG 3942 CACGATGTG HaeII 3516 GGCGCC [GA]GCGC^[CT] 3629 AGCGCC Hin1I 3192 GGCGTC G[GA]^CG[CT]C 3516 GGCGCC KspI 1846 CCGCGG CCGC^GG 2885 CCGCGG NaeI 142 GCCGGC GCC^GGC 5487 GCCGGC NgoMIV 142 GCCGGC G^CCGGC 5487 GCCGGC PmaCI 3009 CACGTG CAC^GTG 6014 CACGTG PmlI 3009 CACGTG CAC^GTG 6014 CACGTG RcaI 1220 TCATGA T^CATGA 1698 TCATGA SacII 1846 CCGCGG CCGC^GG 2885 CCGCGG SapI 4556 GAAGAGC GCTCTTC(1/4) 1605 GCTCTTC SexAI 1174 ACCTGGT A^CC[AT]GGT 3988 ACCAGGT SpeI 4155 ACTAGT A^CTAGT 5195 ACTAGT TaqI 1237 TCGA T^CGA 3674 TCGA TthHB8I 1237 TCGA T^CGA 3674 TCGA ######################################### # 3 OR MORE CUT ENZYMES ( 133 enzymes) # ######################################### AccII AciI AfaI AluI AlwI AlwNI AocI ApaI Asp700I AspHI AvaI AvaII BalI BanI BanII BbsI BbvCI BbvI BcnI BfaI BglI BmyI BpmI BpuAI BsaJI BseRI BsgI BsiEI BsiHKAI BsiYI BslI BsmAI BsmBI BsmFI BsoBI Bsp1286I Bsp1407I BspMI BsrFI BsrGI BsrI BssKI BssSI BstF5I BstNI BstUI BstXI BstYI Bsu36I Cac8I CfoI Cfr10I Cfr13I DdeI DpnII DraII DsaI EaeI EagI EarI EclXI Eco52I Eco81I EcoNI EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeIII HapII HgaI HhaI HinP1I HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MluNI MnlI MscI MseI MslI MspA1I MspI MvaI MvnI MwoI NciI NcoI NdeII NlaIII NlaIV NspI PflMI PleI PpuMI PstI PvuII RsaI SacI Sau3AI Sau96I SbfI ScrFI SfaNI SfcI SmaI SmlI SphI Sse8387I SspBI StyI TfiI Tru9I TseI Tsp45I Tsp509I TspRI Van91I XcmI XhoII XmaCI XmaI XmnI