##################################
# NO CUT ENZYMES ( 79 enzymes) #
##################################
AatII Acc65I AflII AflIII AgeI
Alw44I ApaLI AscI AseI AsnI
Asp700I Asp718I AvaI AviII AvrII
BbvCI BcgI BfrI BlnI BlpI
Bpu1102I BsaBI BsiEI BsiWI BsoBI
BspDI BspLU11I BsrBI BsrFI BssHII
BssSI BstBI BstXI CelII Cfr10I
ClaI CpoI DraIII EagI EclXI
Eco52I EcoRV EcoT22I FseI FspI
HindIII KpnI KspI MamI MluI
NaeI NdeI NgoMIV NheI NotI
NruI NsiI NspV PacI PaeR7I
PinAI PshBI PvuI RsrII SacII
SalI ScaI SfiI SfuI SgrAI
SmaI SnaBI SpeI SphI SwaI
XhoI XmaCI XmaI XmnI
###################################
# ONE CUT ENZYMES ( 49 enzymes) #
###################################
AccIII 3060 TCCGGA T^CCGGA
AhdI 1232 GACTTGGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Aor51HI 376 AGCGCT AGC^GCT
AspEI 1232 GACTTGGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BbeI 1095 GGCGCC GGCGC^C
BbrPI 716 CACGTG CAC^GTG
BciVI 2338 GGATAC GTATCC(6/5)
BclI 978 TGATCA T^GATCA
BglI 120 GCCCCACAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 1352 AGATCT A^GATCT
BseAI 3060 TCCGGA T^CCGGA
BsmBI 742 CGTCTC CGTCTC(1/5)
Bsp1407I 162 TGTACA T^GTACA
BspEI 3060 TCCGGA T^CCGGA
BsrDI 1080 GCAATG GCAATG(2/0)
BsrGI 162 TGTACA T^GTACA
Bst1107I 1247 GTATAC GTA^TAC
BstAPI 565 GCAGGTGGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 1003 GGTCACC G^GT[ACGT]ACC
BstPI 1003 GGTCACC G^GT[ACGT]ACC
BstZ17I 1247 GTATAC GTA^TAC
DsaI 2930 CCATGG C^C[GA][CT]GG
Eam1105I 1232 GACTTGGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 376 AGCGCT AGC^GCT
EcoO65I 1003 GGTCACC G^GT[ACGT]ACC
FbaI 978 TGATCA T^GATCA
HincII 1852 GTTAAC GT[CT]^[GA]AC
HindII 1852 GTTAAC GT[CT]^[GA]AC
HpaI 1852 GTTAAC GTT^AAC
KasI 1095 GGCGCC G^GCGCC
MfeI 1739 CAATTG C^AATTG
MroI 3060 TCCGGA T^CCGGA
MunI 1739 CAATTG C^AATTG
NarI 1095 GGCGCC GG^CGCC
NcoI 2930 CCATGG C^CATGG
NspI 267 ACATGC [GA]CATG^[CT]
PmaCI 716 CACGTG CAC^GTG
PmeI 1932 GTTTAAAC GTTT^AAAC
PmlI 716 CACGTG CAC^GTG
SacI 431 GAGCTC GAGCT^C
SapI 649 GAAGAGC GCTCTTC(1/4)
SbfI 1225 CCTGCAGG CCTGCA^GG
SfoI 1095 GGCGCC GGC^GCC
SmlI 2872 CTCAAG C^T[CT][GA]AG
Sse8387I 1225 CCTGCAGG CCTGCA^GG
SspBI 162 TGTACA T^GTACA
SspI 257 AATATT AAT^ATT
TfiI 902 GATTC G^A[AT]TC
XbaI 1345 TCTAGA T^CTAGA
###################################
# TWO CUT ENZYMES ( 23 enzymes) #
###################################
AccI 1178 GTCTAC GT^[AC][GT]AC
1247 GTATAC
AclI 2113 AACGTT AA^CGTT
3097 AACGTT
AocI 1 CCTGAGG CC^T[ACGT]AGG
205 CCTGAGG
BalI 525 TGGCCA TGG^CCA
618 TGGCCA
BamHI 1191 GGATCC G^GATCC
1536 GGATCC
BsaWI 389 ACCGGA [AT]^CCGG[AT]
3060 TCCGGA
BsmI 1788 GCATTC GAATGC(1/-1)
2977 GCATTC
BspHI 645 TCATGA T^CATGA
2861 TCATGA
Bsu36I 1 CCTGAGG CC^T[ACGT]AGG
205 CCTGAGG
DraI 1933 TTTAAA TTT^AAA
2186 TTTAAA
DrdI 2291 GACTCATGGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
2508 GACTTATAAGTC
Eco81I 1 CCTGAGG CC^T[ACGT]AGG
205 CCTGAGG
EcoRI 1046 GAATTC G^AATTC
2262 GAATTC
FauI 289 CCCGC CCCGC(4/6)
306 CCCGC
HaeII 376 AGCGCT [GA]GCGC^[CT]
1095 GGCGCC
HgaI 466 GACGC GACGC(5/10)
1295 GACGC
MluNI 525 TGGCCA TGG^CCA
618 TGGCCA
MscI 525 TGGCCA TGG^CCA
618 TGGCCA
PshAI 997 GACACAGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
2037 GACAGCTGTC
Psp1406I 2113 AACGTT AA^CGTT
3097 AACGTT
RcaI 645 TCATGA T^CATGA
2861 TCATGA
TaqI 1282 TCGA T^CGA
2910 TCGA
TthHB8I 1282 TCGA T^CGA
2910 TCGA
#########################################
# 3 OR MORE CUT ENZYMES ( 107 enzymes) #
#########################################
AccII AciI AcsI AcyI AfaI
AluI AlwI AlwNI ApaI ApoI
AspHI AspI AvaII BanI BanII
BbsI BbvI BcnI BfaI BmyI
BpmI BpuAI BsaAI BsaHI BsaI
BsaJI BseRI BsgI BsiHKAI BsiYI
BslI BsmAI BsmFI Bsp1286I BspMI
BsrI BssKI BstF5I BstNI BstUI
BstYI Cac8I CfoI Cfr13I DdeI
DpnII DraII EaeI EarI EcoNI
EcoO109I EcoRII EcoT14I Fnu4HI FokI
HaeIII HapII HhaI Hin1I HinP1I
HinfI HpaII HphI ItaI Ksp632I
MaeI MaeII MaeIII MboI MboII
MflI MnlI MseI MslI MspA1I
MspI MvaI MvnI MwoI NciI
NdeII NlaIII NlaIV PflFI PflMI
PleI PpuMI PstI PvuII RsaI
Sau3AI Sau96I ScrFI SexAI SfaNI
SfcI StuI StyI Tru9I TseI
Tsp45I Tsp509I TspRI Tth111I Van91I
XcmI XhoII