##################################
# NO CUT ENZYMES ( 67 enzymes) #
##################################
AatII AccI AclI AflII AgeI
Alw44I ApaLI AscI AseI AsnI
Asp700I BciVI BclI BfrI BsaBI
BsiWI Bsp1407I BspDI BsrGI Bst1107I
BstBI BstEII BstPI BstZ17I ClaI
DraIII DrdI EcoO65I EcoRI EcoRV
EcoT22I FbaI FseI HindIII HpaI
KspI MamI MfeI MluI MunI
NdeI NheI NotI NruI NsiI
NspV PacI PinAI PmeI PshBI
Psp1406I PvuI SacII SalI SbfI
SexAI SfiI SfuI SgrAI SnaBI
SpeI SphI Sse8387I SspBI SspI
SwaI XmnI
###################################
# ONE CUT ENZYMES ( 29 enzymes) #
###################################
AcsI 3008 AAATTT [GA]^AATT[CT]
AhdI 5778 GACTTTGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
ApoI 3008 AAATTT [GA]^AATT[CT]
AspEI 5778 GACTTTGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AviII 1968 TGCGCA TGC^GCA
BamHI 462 GGATCC G^GATCC
BbsI 3187 GAAGAC GAAGAC(2/6)
BcgI 4533 CGATTCTCTTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BlpI 2700 GCTTAGC GC^T[ACGT]AGC
Bpu1102I 2700 GCTTAGC GC^T[ACGT]AGC
BpuAI 3187 GAAGAC GAAGAC(2/6)
BspHI 852 TCATGA T^CATGA
BspLU11I 564 ACATGT A^CATGT
BssSI 5031 CTCGTG CACGAG(-5/-1)
CelII 2700 GCTTAGC GC^T[ACGT]AGC
CpoI 252 CGGTCCG CG^G[AT]CCG
EagI 662 CGGCCG C^GGCCG
Eam1105I 5778 GACTTTGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EclXI 662 CGGCCG C^GGCCG
Eco52I 662 CGGCCG C^GGCCG
FspI 1968 TGCGCA TGC^GCA
HincII 545 GTCAAC GT[CT]^[GA]AC
HindII 545 GTCAAC GT[CT]^[GA]AC
PaeR7I 3066 CTCGAG C^TCGAG
RcaI 852 TCATGA T^CATGA
RsrII 252 CGGTCCG CG^G[AT]CCG
ScaI 690 AGTACT AGT^ACT
XbaI 616 TCTAGA T^CTAGA
XhoI 3066 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 18 enzymes) #
###################################
Acc65I 2187 GGTACC G^GTACC
4358 GGTACC
Asp718I 2187 GGTACC G^GTACC
4358 GGTACC
AvrII 4838 CCTAGG C^CTAGG
5813 CCTAGG
BbrPI 136 CACGTG CAC^GTG
901 CACGTG
BglII 678 AGATCT A^GATCT
1473 AGATCT
BlnI 4838 CCTAGG C^CTAGG
5813 CCTAGG
BsiEI 610 CGACCG CG[GA][CT]^CG
662 CGGCCG
BsmBI 5672 CGTCTC CGTCTC(1/5)
4615 GAGACG
BsmI 1930 GAATGC GAATGC(1/-1)
3102 GCATTC
BsrDI 4239 CATTGC GCAATG(2/0)
2889 GCAATG
DraI 3005 TTTAAA TTT^AAA
3213 TTTAAA
KpnI 2187 GGTACC GGTAC^C
4358 GGTACC
NaeI 3836 GCCGGC GCC^GGC
5586 GCCGGC
NgoMIV 3836 GCCGGC G^CCGGC
5586 GCCGGC
NspI 564 ACATGT [GA]CATG^[CT]
1260 ACATGC
PmaCI 136 CACGTG CAC^GTG
901 CACGTG
PmlI 136 CACGTG CAC^GTG
901 CACGTG
PshAI 3553 GACAGGAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
3862 GACATTAGTC
#########################################
# 3 OR MORE CUT ENZYMES ( 144 enzymes) #
#########################################
AccII AccIII AciI AcyI AfaI
AflIII AluI AlwI AlwNI AocI
Aor51HI ApaI AspHI AspI AvaI
AvaII BalI BanI BanII BbeI
BbvCI BbvI BcnI BfaI BglI
BmyI BpmI BsaAI BsaHI BsaI
BsaJI BsaWI BseAI BseRI BsgI
BsiHKAI BsiYI BslI BsmAI BsmFI
BsoBI Bsp1286I BspEI BspMI BsrBI
BsrFI BsrI BssHII BssKI BstAPI
BstF5I BstNI BstUI BstXI BstYI
Bsu36I Cac8I CfoI Cfr10I Cfr13I
DdeI DpnII DraII DsaI EaeI
EarI Eco47III Eco81I EcoNI EcoO109I
EcoRII EcoT14I FauI Fnu4HI FokI
HaeII HaeIII HapII HgaI HhaI
Hin1I HinP1I HinfI HpaII HphI
ItaI KasI Ksp632I MaeI MaeII
MaeIII MboI MboII MflI MluNI
MnlI MroI MscI MseI MslI
MspA1I MspI MvaI MvnI MwoI
NarI NciI NcoI NdeII NlaIII
NlaIV PflFI PflMI PleI PpuMI
PstI PvuII RsaI SacI SapI
Sau3AI Sau96I ScrFI SfaNI SfcI
SfoI SmaI SmlI StuI StyI
TaqI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI Tth111I TthHB8I Van91I
XcmI XhoII XmaCI XmaI