##################################
# NO CUT ENZYMES (   58 enzymes) #
##################################

AatII       Acc65I      AccIII      AflII       AgeI        
AocI        Aor51HI     AscI        Asp718I     AvrII       
BbvCI       BciVI       BfrI        BglII       BlnI        
BsaWI       BseAI       BsiWI       BspEI       BsrDI       
BstBI       BstEII      BstPI       Bsu36I      CpoI        
DrdI        Eco47III    Eco81I      EcoO65I     EcoRV       
FseI        KpnI        MfeI        MluI        MroI        
MunI        NaeI        NgoMIV      NheI        NotI        
NruI        NspV        PaeR7I      PinAI       PmeI        
PvuI        RsrII       SalI        SbfI        ScaI        
SexAI       SfiI        SfuI        SgrAI       SnaBI       
Sse8387I    SwaI        XhoI        


###################################
# ONE CUT ENZYMES (   45 enzymes) #
###################################

AhdI         3139  GACAACTAGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
ApaI          174  GGGCCC               GGGCC^C
AseI         3131  ATTAAT               AT^TAAT
AsnI         3131  ATTAAT               AT^TAAT
Asp700I      4900  GAATTCTTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspEI        3139  GACAACTAGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AvaI         1060  CCCGGG               C^[CT]CG[GA]G
AviII         546  TGCGCA               TGC^GCA
BclI         2501  TGATCA               T^GATCA
BlpI         5037  GCTGAGC              GC^T[ACGT]AGC
Bpu1102I     5037  GCTGAGC              GC^T[ACGT]AGC
BsaBI        1711  GATGGACATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsiEI         817  CGGCCG               CG[GA][CT]^CG
BsmBI          76  CGTCTC               CGTCTC(1/5)
BsoBI        1060  CCCGGG               C^[CT]CG[GA]G
BspDI        5250  ATCGAT               AT^CGAT
BspHI        4706  TCATGA               T^CATGA
BssSI        1498  CTCGTG               CACGAG(-5/-1)
Bst1107I     2038  GTATAC               GTA^TAC
BstZ17I      2038  GTATAC               GTA^TAC
CelII        5037  GCTGAGC              GC^T[ACGT]AGC
ClaI         5250  ATCGAT               AT^CGAT
DraIII        231  CACCGGGTG            CAC[ACGT][ACGT][ACGT]^GTG
EagI          817  CGGCCG               C^GGCCG
Eam1105I     3139  GACAACTAGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EclXI         817  CGGCCG               C^GGCCG
Eco52I        817  CGGCCG               C^GGCCG
EcoT22I      2825  ATGCAT               ATGCA^T
FbaI         2501  TGATCA               T^GATCA
FspI          546  TGCGCA               TGC^GCA
HpaI         1741  GTTAAC               GTT^AAC
MamI         1711  GATGGACATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
NsiI         2825  ATGCAT               ATGCA^T
PacI         5536  TTAATTAA             TTAAT^TAA
PshAI        3578  GACTGGAGTC           GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PshBI        3131  ATTAAT               AT^TAAT
RcaI         4706  TCATGA               T^CATGA
SacI         1186  GAGCTC               GAGCT^C
SmaI         1060  CCCGGG               CCC^GGG
SpeI         3143  ACTAGT               A^CTAGT
SphI         5173  GCATGC               GCATG^C
StuI         1321  AGGCCT               AGG^CCT
XmaCI        1060  CCCGGG               C^CCGGG
XmaI         1060  CCCGGG               C^CCGGG
XmnI         4900  GAATTCTTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   34 enzymes) #
###################################

AclI         1979  AACGTT               AA^CGTT
             5111  AACGTT              
Alw44I        603  GTGCAC               G^TGCAC
             5071  GTGCAC              
ApaLI         603  GTGCAC               G^TGCAC
             5071  GTGCAC              
AspI          334  GACCGAGTC            GAC[ACGT]^[ACGT][ACGT]GTC
             5194  GACTCTGTC           
BbeI          526  GGCGCC               GGCGC^C
              754  GGCGCC              
BbrPI        1030  CACGTG               CAC^GTG
             5361  CACGTG              
BcgI          474  GCAGCGCCCTCG         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
             5242  GCACGCCCATCG        
BglI           94  GCCACCCCGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
              746  GCCGCGGGGGC         
BsaAI        1030  CACGTG               [CT]AC^GT[GA]
             5361  CACGTG              
BseRI          88  CTCCTC               GAGGAG(10/8)
              431  CTCCTC              
Bsp1407I     1839  TGTACA               T^GTACA
             2261  TGTACA              
BspLU11I     3560  ACATGT               A^CATGT
             5732  ACATGT              
BsrFI         316  ACCGGC               [GA]^CCGG[CT]
             1303  ACCGGC              
BsrGI        1839  TGTACA               T^GTACA
             2261  TGTACA              
Cfr10I        316  ACCGGC               [GA]^CCGG[CT]
             1303  ACCGGC              
EcoRI        2308  GAATTC               G^AATTC
             4900  GAATTC              
HincII       1741  GTTAAC               GT[CT]^[GA]AC
             3991  GTTGAC              
HindII       1741  GTTAAC               GT[CT]^[GA]AC
             3991  GTTGAC              
HindIII      4667  AAGCTT               A^AGCTT
             5962  AAGCTT              
KasI          526  GGCGCC               G^GCGCC
              754  GGCGCC              
NarI          526  GGCGCC               GG^CGCC
              754  GGCGCC              
NcoI          489  CCATGG               C^CATGG
             4498  CCATGG              
PflFI         334  GACCGAGTC            GAC[ACGT]^[ACGT][ACGT]GTC
             5194  GACTCTGTC           
PflMI        1609  CCAGCCAGTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             3149  CCAAGCACTGG         
PmaCI        1030  CACGTG               CAC^GTG
             5361  CACGTG              
PmlI         1030  CACGTG               CAC^GTG
             5361  CACGTG              
Psp1406I     1979  AACGTT               AA^CGTT
             5111  AACGTT              
SapI         1149  GCTCTTC              GCTCTTC(1/4)
             1188  GCTCTTC             
SfoI          526  GGCGCC               GGC^GCC
              754  GGCGCC              
SmlI         1869  CTTGAG               C^T[CT][GA]AG
             5865  CTCAAG              
SspBI        1839  TGTACA               T^GTACA
             2261  TGTACA              
Tth111I       334  GACCGAGTC            GAC[ACGT]^[ACGT][ACGT]GTC
             5194  GACTCTGTC           
Van91I       1609  CCAGCCAGTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             3149  CCAAGCACTGG         
XbaI         3230  TCTAGA               T^CTAGA
             4133  TCTAGA              


#########################################
# 3 OR MORE CUT ENZYMES (  121 enzymes) #
#########################################

AccI        AccII       AciI        AcsI        AcyI        
AfaI        AflIII      AluI        AlwI        AlwNI       
ApoI        AspHI       AvaII       BalI        BamHI       
BanI        BanII       BbsI        BbvI        BcnI        
BfaI        BmyI        BpmI        BpuAI       BsaHI       
BsaI        BsaJI       BsgI        BsiHKAI     BsiYI       
BslI        BsmAI       BsmFI       BsmI        Bsp1286I    
BspMI       BsrBI       BsrI        BssHII      BssKI       
BstAPI      BstF5I      BstNI       BstUI       BstXI       
BstYI       Cac8I       CfoI        Cfr13I      DdeI        
DpnII       DraI        DraII       DsaI        EaeI        
EarI        EcoNI       EcoO109I    EcoRII      EcoT14I     
FauI        Fnu4HI      FokI        HaeII       HaeIII      
HapII       HgaI        HhaI        Hin1I       HinP1I      
HinfI       HpaII       HphI        ItaI        Ksp632I     
KspI        MaeI        MaeII       MaeIII      MboI        
MboII       MflI        MluNI       MnlI        MscI        
MseI        MslI        MspA1I      MspI        MvaI        
MvnI        MwoI        NciI        NdeI        NdeII       
NlaIII      NlaIV       NspI        PleI        PpuMI       
PstI        PvuII       RsaI        SacII       Sau3AI      
Sau96I      ScrFI       SfaNI       SfcI        SspI        
StyI        TaqI        TfiI        Tru9I       TseI        
Tsp45I      Tsp509I     TspRI       TthHB8I     XcmI        
XhoII