##################################
# NO CUT ENZYMES ( 100 enzymes) #
##################################
AatII Acc65I AccIII AclI AcyI
AflIII AgeI Alw44I AocI Aor51HI
ApaI ApaLI AscI AseI AsnI
Asp718I AspI AvrII BbeI BbrPI
BbsI BcgI BclI BlnI BpuAI
BsaAI BsaBI BsaHI BseAI BsiEI
BsiWI Bsp1407I BspEI BspHI BspLU11I
BspMI BsrFI BsrGI BssHII Bst1107I
BstAPI BstEII BstPI BstZ17I Bsu36I
Cfr10I CpoI EagI EclXI Eco47III
Eco52I Eco81I EcoNI EcoO65I EcoRV
FbaI FseI HaeII Hin1I HpaI
KasI KpnI KspI MamI MluI
MroI NaeI NarI NgoMIV NotI
NruI PacI PaeR7I PflFI PinAI
PmaCI PmeI PmlI PshAI PshBI
Psp1406I PstI PvuI RcaI RsrII
SacII SalI SbfI ScaI SexAI
SfoI SgrAI SnaBI SpeI Sse8387I
SspBI SspI SwaI Tth111I XhoI
###################################
# ONE CUT ENZYMES ( 50 enzymes) #
###################################
AccI 2238 GTCTAC GT^[AC][GT]AC
AccII 3215 CGCG CG^CG
AflII 1479 CTTAAG C^TTAAG
AhdI 7 GACAGCTTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspEI 7 GACAGCTTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AvaI 3183 CCCGGG C^[CT]CG[GA]G
AviII 860 TGCGCA TGC^GCA
BbvCI 3026 GCTGAGG CCTCAGC(-5/-2)
BciVI 891 GTATCC GTATCC(6/5)
BfrI 1479 CTTAAG C^TTAAG
BglI 1963 GCCACGCAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlpI 2698 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 2698 GCTCAGC GC^T[ACGT]AGC
BsaI 3059 GAGACC GGTCTC(1/5)
BsaWI 1999 ACCGGA [AT]^CCGG[AT]
BsmBI 1607 GAGACG CGTCTC(1/5)
BsoBI 3183 CCCGGG C^[CT]CG[GA]G
BspDI 865 ATCGAT AT^CGAT
BsrBI 537 CCGCTC CCGCTC(-3/-3)
BsrDI 45 GCAATG GCAATG(2/0)
BstBI 809 TTCGAA TT^CGAA
BstUI 3215 CGCG CG^CG
CelII 2698 GCTCAGC GC^T[ACGT]AGC
ClaI 865 ATCGAT AT^CGAT
DraI 264 TTTAAA TTT^AAA
DraII 2801 GGGACCT [GA]G^G[ACGT]CC[CT]
DrdI 720 GACATTTCCGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eam1105I 7 GACAGCTTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoO109I 2801 GGGACCT [GA]G^G[ACGT]CC[CT]
EcoT22I 1728 ATGCAT ATGCA^T
FspI 860 TGCGCA TGC^GCA
HgaI 401 GACGC GACGC(5/10)
HindIII 452 AAGCTT A^AGCTT
MspA1I 116 CAGCTG C[AC]G^C[GT]G
MvnI 3215 CGCG CG^CG
NdeI 2871 CATATG CA^TATG
NheI 3071 GCTAGC G^CTAGC
NsiI 1728 ATGCAT ATGCA^T
NspV 809 TTCGAA TT^CGAA
PpuMI 2801 GGGACCT [GA]G^G[AT]CC[CT]
PvuII 116 CAGCTG CAG^CTG
SacI 2245 GAGCTC GAGCT^C
SfcI 668 CTACAG C^T[GA][CT]AG
SfiI 1962 GGCCACGCAGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfuI 809 TTCGAA TT^CGAA
SmaI 3183 CCCGGG CCC^GGG
SphI 3128 GCATGC GCATG^C
XbaI 2787 TCTAGA T^CTAGA
XmaCI 3183 CCCGGG C^CCGGG
XmaI 3183 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 22 enzymes) #
###################################
AlwNI 1696 CAGCCCCTG CAG[ACGT][ACGT][ACGT]^CTG
1718 CAGTGGCTG
AspHI 2245 GAGCTC G[AT]GC[AT]^C
2926 GTGCTC
BalI 1739 TGGCCA TGG^CCA
1961 TGGCCA
BamHI 2002 GGATCC G^GATCC
2449 GGATCC
BanI 1215 GGCACC G^G[CT][GA]CC
1618 GGTGCC
BpmI 838 CTCCAG CTGGAG(16/14)
1543 CTGGAG
BsgI 23 GTGCAG GTGCAG(16/14)
1310 GTGCAG
BsiHKAI 2245 GAGCTC G[AT]GC[AT]^C
2926 GTGCTC
DraIII 1207 CACTTTGTG CAC[ACGT][ACGT][ACGT]^GTG
2920 CACTCAGTG
EaeI 1739 TGGCCA [CT]^GGCC[GA]
1961 TGGCCA
FauI 705 GCGGG CCCGC(4/6)
3032 GCGGG
HincII 427 GTCAAC GT[CT]^[GA]AC
879 GTCAAC
HindII 427 GTCAAC GT[CT]^[GA]AC
879 GTCAAC
MfeI 155 CAATTG C^AATTG
1562 CAATTG
MluNI 1739 TGGCCA TGG^CCA
1961 TGGCCA
MscI 1739 TGGCCA TGG^CCA
1961 TGGCCA
MslI 547 CATGGAGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
957 CATCCTGGTG
MunI 155 CAATTG C^AATTG
1562 CAATTG
PflMI 1742 CCAACCACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2356 CCAAGGTTTGG
SapI 1524 GAAGAGC GCTCTTC(1/4)
2247 GCTCTTC
StuI 1787 AGGCCT AGG^CCT
2552 AGGCCT
Van91I 1742 CCAACCACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2356 CCAAGGTTTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 86 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
ApoI Asp700I AvaII BanII BbvI
BcnI BfaI BglII BmyI BsaJI
BseRI BsiYI BslI BsmAI BsmFI
BsmI Bsp1286I BsrI BssKI BssSI
BstF5I BstNI BstXI BstYI Cac8I
CfoI Cfr13I DdeI DpnII DsaI
EarI EcoRI EcoRII EcoT14I Fnu4HI
FokI HaeIII HapII HhaI HinP1I
HinfI HpaII HphI ItaI Ksp632I
MaeI MaeII MaeIII MboI MboII
MflI MnlI MseI MspI MvaI
MwoI NciI NcoI NdeII NlaIII
NlaIV NspI PleI RsaI Sau3AI
Sau96I ScrFI SfaNI SmlI StyI
TaqI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI TthHB8I XcmI XhoII
XmnI