##################################
# NO CUT ENZYMES ( 70 enzymes) #
##################################
AatII Acc65I AclI AcsI AgeI
Alw44I Aor51HI ApaLI ApoI AscI
AseI AsnI Asp700I Asp718I AvrII
BciVI BlnI BlpI Bpu1102I BsaBI
BsiWI Bsp1407I BspDI BsrBI BsrGI
BssHII Bst1107I BstAPI BstBI BstZ17I
CelII ClaI CpoI DraI Eco47III
EcoRI EcoRV EcoT22I FseI HindIII
HpaI KpnI MamI MluI NheI
NruI NsiI NspV PacI PaeR7I
PinAI PmeI PshAI PshBI Psp1406I
PvuI RsrII SalI SbfI ScaI
SfuI SnaBI SpeI SphI Sse8387I
SspBI SwaI XbaI XhoI XmnI
###################################
# ONE CUT ENZYMES ( 54 enzymes) #
###################################
AccI 790 GTAGAC GT^[AC][GT]AC
AccIII 154 TCCGGA T^CCGGA
AflII 3066 CTTAAG C^TTAAG
AflIII 3142 ACATGT A^C[GA][CT]GT
AviII 1891 TGCGCA TGC^GCA
BamHI 603 GGATCC G^GATCC
BbeI 2889 GGCGCC GGCGC^C
BbrPI 1228 CACGTG CAC^GTG
BcgI 3461 GCAGGAGAATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI 3084 TGATCA T^GATCA
BfrI 3066 CTTAAG C^TTAAG
BglII 818 AGATCT A^GATCT
BsaAI 1228 CACGTG [CT]AC^GT[GA]
BsaWI 154 TCCGGA [AT]^CCGG[AT]
BseAI 154 TCCGGA T^CCGGA
BsiEI 2 CGGCCG CG[GA][CT]^CG
BspEI 154 TCCGGA T^CCGGA
BspHI 2798 TCATGA T^CATGA
BspLU11I 3142 ACATGT A^CATGT
BsrDI 709 CATTGC GCAATG(2/0)
BssSI 2323 CACGAG CACGAG(-5/-1)
BstEII 220 GGTTACC G^GT[ACGT]ACC
BstPI 220 GGTTACC G^GT[ACGT]ACC
EagI 2 CGGCCG C^GGCCG
EclXI 2 CGGCCG C^GGCCG
Eco52I 2 CGGCCG C^GGCCG
EcoO65I 220 GGTTACC G^GT[ACGT]ACC
FbaI 3084 TGATCA T^GATCA
FspI 1891 TGCGCA TGC^GCA
HgaI 1560 GACGC GACGC(5/10)
HincII 3148 GTTGAC GT[CT]^[GA]AC
HindII 3148 GTTGAC GT[CT]^[GA]AC
KasI 2889 GGCGCC G^GCGCC
KspI 5 CCGCGG CCGC^GG
MroI 154 TCCGGA T^CCGGA
NarI 2889 GGCGCC GG^CGCC
NdeI 1268 CATATG CA^TATG
NotI 1 GCGGCCGC GC^GGCCGC
PmaCI 1228 CACGTG CAC^GTG
PmlI 1228 CACGTG CAC^GTG
PpuMI 3316 AGGTCCC [GA]G^G[AT]CC[CT]
RcaI 2798 TCATGA T^CATGA
SacI 2730 GAGCTC GAGCT^C
SacII 5 CCGCGG CCGC^GG
SexAI 1986 ACCTGGT A^CC[AT]GGT
SfiI 66 GGCCATGGGGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfoI 2889 GGCGCC GGC^GCC
SgrAI 2035 CGCCGGCG C[GA]^CCGG[CT]G
SmaI 2941 CCCGGG CCC^GGG
SspI 1144 AATATT AAT^ATT
StuI 3103 AGGCCT AGG^CCT
XcmI 1601 CCAGCAGCAGCTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmaCI 2941 CCCGGG C^CCGGG
XmaI 2941 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 24 enzymes) #
###################################
AcyI 1560 GACGCC G[GA]^CG[CT]C
2889 GGCGCC
AlwNI 2114 CAGCCTCTG CAG[ACGT][ACGT][ACGT]^CTG
2166 CAGCTGCTG
AocI 2422 CCTTAGG CC^T[ACGT]AGG
3119 CCTAAGG
AspI 793 GACGGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
1657 GACACCGTC
BsaHI 1560 GACGCC G[GA]^CG[CT]C
2889 GGCGCC
BspMI 1286 ACCTGC ACCTGC(4/8)
2520 ACCTGC
BsrFI 86 GCCGGC [GA]^CCGG[CT]
2036 GCCGGC
Bsu36I 2422 CCTTAGG CC^T[ACGT]AGG
3119 CCTAAGG
Cfr10I 86 GCCGGC [GA]^CCGG[CT]
2036 GCCGGC
DraIII 595 CACACCGTG CAC[ACGT][ACGT][ACGT]^GTG
3171 CACTGGGTG
DrdI 1560 GACGCCTCTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
2008 GACAATGCCGTC
Eco81I 2422 CCTTAGG CC^T[ACGT]AGG
3119 CCTAAGG
Hin1I 1560 GACGCC G[GA]^CG[CT]C
2889 GGCGCC
MfeI 2511 CAATTG C^AATTG
3216 CAATTG
MunI 2511 CAATTG C^AATTG
3216 CAATTG
NaeI 86 GCCGGC GCC^GGC
2036 GCCGGC
NgoMIV 86 GCCGGC G^CCGGC
2036 GCCGGC
PflFI 793 GACGGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
1657 GACACCGTC
PflMI 594 CCACACCGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3166 CCAAGCACTGG
SapI 381 GAAGAGC GCTCTTC(1/4)
1123 GAAGAGC
TaqI 2814 TCGA T^CGA
3552 TCGA
Tth111I 793 GACGGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
1657 GACACCGTC
TthHB8I 2814 TCGA T^CGA
3552 TCGA
Van91I 594 CCACACCGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3166 CCAAGCACTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 110 enzymes) #
#########################################
AccII AciI AfaI AhdI AluI
AlwI ApaI AspEI AspHI AvaI
AvaII BalI BanI BanII BbsI
BbvCI BbvI BcnI BfaI BglI
BmyI BpmI BpuAI BsaI BsaJI
BseRI BsgI BsiHKAI BsiYI BslI
BsmAI BsmBI BsmFI BsmI BsoBI
Bsp1286I BsrI BssKI BstF5I BstNI
BstUI BstXI BstYI Cac8I CfoI
Cfr13I DdeI DpnII DraII DsaI
EaeI Eam1105I EarI EcoNI EcoO109I
EcoRII EcoT14I FauI Fnu4HI FokI
HaeII HaeIII HapII HhaI HinP1I
HinfI HpaII HphI ItaI Ksp632I
MaeI MaeII MaeIII MboI MboII
MflI MluNI MnlI MscI MseI
MslI MspA1I MspI MvaI MvnI
MwoI NciI NcoI NdeII NlaIII
NlaIV NspI PleI PstI PvuII
RsaI Sau3AI Sau96I ScrFI SfaNI
SfcI SmlI StyI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI XhoII