##################################
# NO CUT ENZYMES ( 97 enzymes) #
##################################
AatII AccIII AclI AcyI AgeI
Alw44I Aor51HI ApaI ApaLI AscI
AseI AsnI AspI AvaI AviII
BbeI BbrPI BcgI BlpI Bpu1102I
BsaAI BsaBI BsaHI BsaI BseAI
BseRI BsgI BsiWI BsmBI BsoBI
BspEI BsrBI BsrFI BssHII BssSI
Bst1107I BstBI BstZ17I CelII Cfr10I
CpoI DrdI EagI EclXI Eco47III
Eco52I FseI FspI HaeII HgaI
Hin1I HpaI KasI KspI MamI
MfeI MluI MroI MunI NaeI
NarI NdeI NgoMIV NotI NruI
NspV PacI PaeR7I PflFI PinAI
PmaCI PmeI PmlI PshBI Psp1406I
PvuI RsrII SacII SalI SbfI
ScaI SexAI SfiI SfoI SfuI
SgrAI SmaI SnaBI Sse8387I SspI
StuI SwaI Tth111I XbaI XhoI
XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 51 enzymes) #
###################################
Acc65I 2778 GGTACC G^GTACC
AccI 1339 GTCTAC GT^[AC][GT]AC
AccII 781 CGCG CG^CG
AflII 2051 CTTAAG C^TTAAG
AocI 3061 CCTCAGG CC^T[ACGT]AGG
Asp700I 424 GAAGAAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 2778 GGTACC G^GTACC
AvrII 3069 CCTAGG C^CTAGG
BalI 1491 TGGCCA TGG^CCA
BamHI 1415 GGATCC G^GATCC
BanII 562 GAGCTC G[GA]GC[CT]^C
BbsI 1223 GTCTTC GAAGAC(2/6)
BbvCI 28 GCTGAGG CCTCAGC(-5/-2)
BclI 825 TGATCA T^GATCA
BfrI 2051 CTTAAG C^TTAAG
BglI 708 GCCTGTCTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 503 AGATCT A^GATCT
BlnI 3069 CCTAGG C^CTAGG
BpuAI 1223 GTCTTC GAAGAC(2/6)
BsaWI 1412 ACCGGA [AT]^CCGG[AT]
BsiEI 474 CGACCG CG[GA][CT]^CG
BspHI 1762 TCATGA T^CATGA
BspLU11I 2587 ACATGT A^CATGT
BspMI 1481 ACCTGC ACCTGC(4/8)
BstEII 3073 GGTCACC G^GT[ACGT]ACC
BstPI 3073 GGTCACC G^GT[ACGT]ACC
BstUI 781 CGCG CG^CG
BstXI 878 CCATGCCTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I 3061 CCTCAGG CC^T[ACGT]AGG
DraIII 142 CACGGTGTG CAC[ACGT][ACGT][ACGT]^GTG
Eco81I 3061 CCTCAGG CC^T[ACGT]AGG
EcoNI 450 CCTGCTTCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 3073 GGTCACC G^GT[ACGT]ACC
EcoRV 1233 GATATC GAT^ATC
EcoT22I 2926 ATGCAT ATGCA^T
FbaI 825 TGATCA T^GATCA
HindIII 1369 AAGCTT A^AGCTT
KpnI 2778 GGTACC GGTAC^C
MluNI 1491 TGGCCA TGG^CCA
MscI 1491 TGGCCA TGG^CCA
MvnI 781 CGCG CG^CG
NsiI 2926 ATGCAT ATGCA^T
PpuMI 3065 AGGACCT [GA]G^G[AT]CC[CT]
PshAI 3067 GACCTAGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PstI 1327 CTGCAG CTGCA^G
PvuII 253 CAGCTG CAG^CTG
RcaI 1762 TCATGA T^CATGA
SacI 562 GAGCTC GAGCT^C
SpeI 2948 ACTAGT A^CTAGT
XcmI 1494 CCAAAATGAATCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmnI 424 GAAGAAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 23 enzymes) #
###################################
AhdI 984 GACTGCCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
2341 GACCTTGGGTC
AspEI 984 GACTGCCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
2341 GACCTTGGGTC
AspHI 562 GAGCTC G[AT]GC[AT]^C
1820 GTGCTC
AvaII 728 GGACC G^G[AT]CC
3066 GGACC
BmyI 562 GAGCTC G[AGT]GC[ACT]^C
1820 GTGCTC
BsiHKAI 562 GAGCTC G[AT]GC[AT]^C
1820 GTGCTC
BsmFI 920 GGGAC GGGAC(10/14)
624 GTCCC
BsmI 834 GAATGC GAATGC(1/-1)
2017 GCATTC
Bsp1286I 562 GAGCTC G[AGT]GC[ACT]^C
1820 GTGCTC
BspDI 376 ATCGAT AT^CGAT
2101 ATCGAT
BstAPI 2 GCATCCTCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
1053 GCATTTTGTGC
ClaI 376 ATCGAT AT^CGAT
2101 ATCGAT
DraII 674 AGGCCCT [GA]G^G[ACGT]CC[CT]
3065 AGGACCT
EaeI 963 TGGCCG [CT]^GGCC[GA]
1491 TGGCCA
Eam1105I 984 GACTGCCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
2341 GACCTTGGGTC
EcoO109I 674 AGGCCCT [GA]G^G[ACGT]CC[CT]
3065 AGGACCT
FauI 1004 CCCGC CCCGC(4/6)
238 GCGGG
HincII 667 GTCAAC GT[CT]^[GA]AC
1935 GTCAAC
HindII 667 GTCAAC GT[CT]^[GA]AC
1935 GTCAAC
MspA1I 236 CAGCGG C[AC]G^C[GT]G
253 CAGCTG
NheI 231 GCTAGC G^CTAGC
513 GCTAGC
TaqI 377 TCGA T^CGA
2102 TCGA
TthHB8I 377 TCGA T^CGA
2102 TCGA
#########################################
# 3 OR MORE CUT ENZYMES ( 87 enzymes) #
#########################################
AciI AcsI AfaI AflIII AluI
AlwI AlwNI ApoI BanI BbvI
BciVI BcnI BfaI BpmI BsaJI
BsiYI BslI BsmAI Bsp1407I BsrDI
BsrGI BsrI BssKI BstF5I BstNI
BstYI Cac8I CfoI Cfr13I DdeI
DpnII DraI DsaI EarI EcoRI
EcoRII EcoT14I Fnu4HI FokI HaeIII
HapII HhaI HinP1I HinfI HpaII
HphI ItaI Ksp632I MaeI MaeII
MaeIII MboI MboII MflI MnlI
MseI MslI MspI MvaI MwoI
NciI NcoI NdeII NlaIII NlaIV
NspI PflMI PleI RsaI SapI
Sau3AI Sau96I ScrFI SfaNI SfcI
SmlI SphI SspBI StyI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
Van91I XhoII