##################################
# NO CUT ENZYMES ( 89 enzymes) #
##################################
AatII Acc65I AccIII AclI AcsI
AflII AflIII Alw44I Aor51HI ApaLI
ApoI AscI AseI AsnI Asp718I
AspI AviII AvrII BfrI BlnI
BsaBI BseAI BsiWI BsmI Bsp1407I
BspDI BspEI BspLU11I BsrDI BsrGI
BstBI ClaI CpoI EarI Eco47III
EcoRI EcoRV EcoT22I FseI FspI
HincII HindII HpaI KpnI Ksp632I
MamI MfeI MluI MroI MslI
MunI NcoI NdeI NheI NotI
NruI NsiI NspV PacI PaeR7I
PflFI PmeI PshAI PshBI Psp1406I
PvuI RsrII SalI SapI SbfI
ScaI SexAI SfiI SfuI SgrAI
SmaI SmlI SnaBI SpeI Sse8387I
SspBI SwaI TaqI Tsp509I Tth111I
TthHB8I XhoI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 33 enzymes) #
###################################
AgeI 3310 ACCGGT A^CCGGT
Asp700I 2682 GAACCCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
BbeI 12 GGCGCC GGCGC^C
BbrPI 3011 CACGTG CAC^GTG
BbvCI 2762 CCTCAGC CCTCAGC(-5/-2)
BclI 2565 TGATCA T^GATCA
BsaAI 3011 CACGTG [CT]AC^GT[GA]
BspHI 173 TCATGA T^CATGA
BsrBI 62 CCGCTC CCGCTC(-3/-3)
BssHII 2449 GCGCGC G^CGCGC
BstEII 3306 GGTGACC G^GT[ACGT]ACC
BstPI 3306 GGTGACC G^GT[ACGT]ACC
DraI 3370 TTTAAA TTT^AAA
DrdI 2335 GACCAAGCCGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoO65I 3306 GGTGACC G^GT[ACGT]ACC
FbaI 2565 TGATCA T^GATCA
HindIII 2937 AAGCTT A^AGCTT
KasI 12 GGCGCC G^GCGCC
KspI 2475 CCGCGG CCGC^GG
MseI 3371 TTAA T^TAA
NaeI 2470 GCCGGC GCC^GGC
NarI 12 GGCGCC GG^CGCC
NgoMIV 2470 GCCGGC G^CCGGC
PinAI 3310 ACCGGT A^CCGGT
PmaCI 3011 CACGTG CAC^GTG
PmlI 3011 CACGTG CAC^GTG
RcaI 173 TCATGA T^CATGA
SacII 2475 CCGCGG CCGC^GG
SfoI 12 GGCGCC GGC^GCC
SphI 3077 GCATGC GCATG^C
SspI 3507 AATATT AAT^ATT
Tru9I 3371 TTAA T^TAA
XmnI 2682 GAACCCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 22 enzymes) #
###################################
AccI 859 GTATAC GT^[AC][GT]AC
3490 GTATAC
AfaI 2528 GTAC GT^AC
3198 GTAC
ApaI 1058 GGGCCC GGGCC^C
3290 GGGCCC
BalI 1351 TGGCCA TGG^CCA
2707 TGGCCA
BcgI 2507 CGAGTGATCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
2370 GCAAAGCCCTCG
BsaWI 2880 TCCGGT [AT]^CCGG[AT]
3310 ACCGGT
BsrFI 2470 GCCGGC [GA]^CCGG[CT]
3310 ACCGGT
BssSI 2711 CACGAG CACGAG(-5/-1)
2703 CTCGTG
Bst1107I 859 GTATAC GTA^TAC
3490 GTATAC
BstAPI 338 GCAAGCCCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
2459 GCAGAGTGTGC
BstZ17I 859 GTATAC GTA^TAC
3490 GTATAC
Cfr10I 2470 GCCGGC [GA]^CCGG[CT]
3310 ACCGGT
DraIII 3041 CACGCAGTG CAC[ACGT][ACGT][ACGT]^GTG
3433 CACTCAGTG
HaeII 12 GGCGCC [GA]GCGC^[CT]
2123 GGCGCT
MluNI 1351 TGGCCA TGG^CCA
2707 TGGCCA
MscI 1351 TGGCCA TGG^CCA
2707 TGGCCA
NspI 1226 ACATGC [GA]CATG^[CT]
3077 GCATGC
PflMI 272 CCACAGGATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3040 CCACGCAGTGG
RsaI 2528 GTAC GT^AC
3198 GTAC
SacI 1481 GAGCTC GAGCT^C
3343 GAGCTC
StuI 2178 AGGCCT AGG^CCT
2605 AGGCCT
Van91I 272 CCACAGGATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3040 CCACGCAGTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 114 enzymes) #
#########################################
AccII AciI AcyI AhdI AluI
AlwI AlwNI AocI AspEI AspHI
AvaI AvaII BamHI BanI BanII
BbsI BbvI BciVI BcnI BfaI
BglI BglII BlpI BmyI BpmI
Bpu1102I BpuAI BsaHI BsaI BsaJI
BseRI BsgI BsiEI BsiHKAI BsiYI
BslI BsmAI BsmBI BsmFI BsoBI
Bsp1286I BspMI BsrI BssKI BstF5I
BstNI BstUI BstXI BstYI Bsu36I
Cac8I CelII CfoI Cfr13I DdeI
DpnII DraII DsaI EaeI EagI
Eam1105I EclXI Eco52I Eco81I EcoNI
EcoO109I EcoRII EcoT14I FauI Fnu4HI
FokI HaeIII HapII HgaI HhaI
Hin1I HinP1I HinfI HpaII HphI
ItaI MaeI MaeII MaeIII MboI
MboII MflI MnlI MspA1I MspI
MvaI MvnI MwoI NciI NdeII
NlaIII NlaIV PleI PpuMI PstI
PvuII Sau3AI Sau96I ScrFI SfaNI
SfcI StyI TfiI TseI Tsp45I
TspRI XbaI XcmI XhoII