##################################
# NO CUT ENZYMES (  131 enzymes) #
##################################

AatII       Acc65I      AccII       AccIII      AclI        
AcyI        AflII       AgeI        Alw44I      Aor51HI     
ApaI        ApaLI       AscI        Asp718I     AspHI       
AspI        AvaI        AviII       AvrII       BanI        
BbeI        BbrPI       BbvCI       BcgI        BcnI        
BfrI        BglI        BlnI        BlpI        Bpu1102I    
BsaBI       BsaHI       BsaI        BsaWI       BseAI       
BsgI        BsiEI       BsiHKAI     BsiWI       BsmBI       
BsoBI       Bsp1407I    BspDI       BspEI       BspHI       
BsrBI       BsrFI       BsrGI       BssHII      BssSI       
Bst1107I    BstAPI      BstEII      BstPI       BstUI       
BstXI       BstZ17I     CelII       CfoI        Cfr10I      
ClaI        CpoI        DraII       EagI        EarI        
EclXI       Eco47III    Eco52I      EcoO109I    EcoO65I     
EcoRI       FseI        FspI        HaeII       HapII       
HgaI        HhaI        Hin1I       HinP1I      HincII      
HindII      HpaI        HpaII       KasI        KpnI        
Ksp632I     KspI        MamI        MluI        MroI        
MspI        MvnI        NaeI        NarI        NciI        
NdeI        NgoMIV      NheI        NotI        NruI        
PacI        PaeR7I      PflFI       PinAI       PmaCI       
PmeI        PmlI        PpuMI       PshAI       Psp1406I    
PvuI        RcaI        RsrII       SacI        SacII       
SalI        SapI        SexAI       SfiI        SfoI        
SgrAI       SmaI        SpeI        SphI        SspBI       
StuI        Tth111I     XbaI        XhoI        XmaCI       
XmaI        


###################################
# ONE CUT ENZYMES (   48 enzymes) #
###################################

AccI         1913  GTAGAC               GT^[AC][GT]AC
AflIII       1695  ACATGT               A^C[GA][CT]GT
AhdI         1101  GACTAAAGGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AocI         2000  CCTAAGG              CC^T[ACGT]AGG
AspEI        1101  GACTAAAGGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AvaII        1283  GGTCC                G^G[AT]CC
BalI         1442  TGGCCA               TGG^CCA
BamHI        1675  GGATCC               G^GATCC
BanII        2802  GAGCCC               G[GA]GC[CT]^C
BclI         2783  TGATCA               T^GATCA
BsmAI        1379  GAGAC                GTCTC(1/5)
BsmI          166  GCATTC               GAATGC(1/-1)
BspLU11I     1695  ACATGT               A^CATGT
BssKI        1351  CCAGG                ^CC[ACGT]GG
BstBI        1710  TTCGAA               TT^CGAA
BstNI        1351  CCAGG                CC^[AT]GG
Bsu36I       2000  CCTAAGG              CC^T[ACGT]AGG
Cfr13I       1283  GGTCC                G^G[ACGT]CC
DraIII       2766  CACTAGGTG            CAC[ACGT][ACGT][ACGT]^GTG
DrdI         1275  GACTATTGGGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EaeI         1442  TGGCCA               [CT]^GGCC[GA]
Eam1105I     1101  GACTAAAGGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I       2000  CCTAAGG              CC^T[ACGT]AGG
EcoNI        3050  CCTTGCAAAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRII       1351  CCAGG                ^CC[AT]GG
EcoT22I      2613  ATGCAT               ATGCA^T
FauI         1735  GCGGG                CCCGC(4/6)
FbaI         2783  TGATCA               T^GATCA
MfeI          768  CAATTG               C^AATTG
MluNI        1442  TGGCCA               TGG^CCA
MscI         1442  TGGCCA               TGG^CCA
MspA1I        192  CAGCTG               C[AC]G^C[GT]G
MunI          768  CAATTG               C^AATTG
MvaI         1351  CCAGG                CC^[AT]GG
NsiI         2613  ATGCAT               ATGCA^T
NspV         1710  TTCGAA               TT^CGAA
PflMI        2765  CCACTAGGTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PvuII         192  CAGCTG               CAG^CTG
Sau96I       1283  GGTCC                G^G[ACGT]CC
SbfI         1586  CCTGCAGG             CCTGCA^GG
ScaI          206  AGTACT               AGT^ACT
ScrFI        1351  CCAGG                CC^[ACGT]GG
SfaNI         535  GATGC                GCATC(5/9)
SfuI         1710  TTCGAA               TT^CGAA
SmlI         2088  CTCAAG               C^T[CT][GA]AG
Sse8387I     1586  CCTGCAGG             CCTGCA^GG
SwaI          267  ATTTAAAT             ATTT^AAAT
Van91I       2765  CCACTAGGTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG


###################################
# TWO CUT ENZYMES (   24 enzymes) #
###################################

AlwNI         909  CAGTAGCTG            CAG[ACGT][ACGT][ACGT]^CTG
             1773  CAGAGGCTG           
AseI          110  ATTAAT               AT^TAAT
             1313  ATTAAT              
AsnI          110  ATTAAT               AT^TAAT
             1313  ATTAAT              
BbsI         1567  GAAGAC               GAAGAC(2/6)
             2910  GAAGAC              
BciVI         761  GGATAC               GTATCC(6/5)
              128  GTATCC              
BglII         291  AGATCT               A^GATCT
             1266  AGATCT              
BmyI         2802  GAGCCC               G[AGT]GC[ACT]^C
             3059  GGGCAC              
BpmI          295  CTGGAG               CTGGAG(16/14)
             2305  CTGGAG              
BpuAI        1567  GAAGAC               GAAGAC(2/6)
             2910  GAAGAC              
BsaAI        2571  TACGTA               [CT]AC^GT[GA]
             2930  TACGTA              
BsmFI        2241  GGGAC                GGGAC(10/14)
             2017  GTCCC               
Bsp1286I     2802  GAGCCC               G[AGT]GC[ACT]^C
             3059  GGGCAC              
BspMI         930  ACCTGC               ACCTGC(4/8)
             1585  ACCTGC              
EcoRV          56  GATATC               GAT^ATC
              503  GATATC              
EcoT14I       405  CCATGG               C^C[AT][AT]GG
              893  CCATGG              
HaeIII       1349  GGCC                 GG^CC
             1443  GGCC                
NcoI          405  CCATGG               C^CATGG
              893  CCATGG              
PleI           50  GACTC                GAGTC(4/5)
             2951  GACTC               
PshBI         110  ATTAAT               AT^TAAT
             1313  ATTAAT              
PstI          932  CTGCAG               CTGCA^G
             1587  CTGCAG              
SnaBI        2571  TACGTA               TAC^GTA
             2930  TACGTA              
SspI         1316  AATATT               AAT^ATT
             3087  AATATT              
StyI          405  CCATGG               C^C[AT][AT]GG
              893  CCATGG              
XcmI          470  CCATCTGCATATTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
             1752  CCACTCACTATATGG     


#########################################
# 3 OR MORE CUT ENZYMES (   55 enzymes) #
#########################################

AciI        AcsI        AfaI        AluI        AlwI        
ApoI        Asp700I     BbvI        BfaI        BsaJI       
BseRI       BsiYI       BslI        BsrDI       BsrI        
BstF5I      BstYI       Cac8I       DdeI        DpnII       
DraI        DsaI        Fnu4HI      FokI        HindIII     
HinfI       HphI        ItaI        MaeI        MaeII       
MaeIII      MboI        MboII       MflI        MnlI        
MseI        MslI        MwoI        NdeII       NlaIII      
NlaIV       NspI        RsaI        Sau3AI      SfcI        
TaqI        TfiI        Tru9I       TseI        Tsp45I      
Tsp509I     TspRI       TthHB8I     XhoII       XmnI