##################################
# NO CUT ENZYMES ( 72 enzymes) #
##################################
AclI AflII AhdI Alw44I Aor51HI
ApaLI AscI AseI AsnI Asp700I
AspEI AspI AvrII BbrPI BclI
BfrI BlnI BsiWI Bsp1407I BspDI
BspHI BsrGI Bst1107I BstBI BstZ17I
ClaI CpoI DraI Eam1105I Eco47III
EcoRI EcoT22I FbaI FseI HpaI
MfeI MluI MunI NdeI NheI
NotI NsiI NspV PacI PaeR7I
PflFI PmaCI PmeI PmlI PshAI
PshBI Psp1406I PvuI RcaI RsrII
SalI SbfI ScaI SfiI SfuI
SgrAI SnaBI SpeI Sse8387I SspBI
SspI SwaI Tth111I XbaI XcmI
XhoI XmnI
###################################
# ONE CUT ENZYMES ( 40 enzymes) #
###################################
Acc65I 970 GGTACC G^GTACC
AccIII 401 TCCGGA T^CCGGA
AflIII 1784 ACATGT A^C[GA][CT]GT
AgeI 1933 ACCGGT A^CCGGT
Asp718I 970 GGTACC G^GTACC
BbsI 1927 GTCTTC GAAGAC(2/6)
BcgI 2859 CGACATTTCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 1057 GTATCC GTATCC(6/5)
BlpI 1256 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 1256 GCTGAGC GC^T[ACGT]AGC
BpuAI 1927 GTCTTC GAAGAC(2/6)
BsaAI 3386 CACGTA [CT]AC^GT[GA]
BsaBI 318 GATGCCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BseAI 401 TCCGGA T^CCGGA
BsiEI 21 CGGCCG CG[GA][CT]^CG
BsmI 2882 GAATGC GAATGC(1/-1)
BspEI 401 TCCGGA T^CCGGA
BspLU11I 1784 ACATGT A^CATGT
BsrDI 1877 CATTGC GCAATG(2/0)
BssSI 1128 CTCGTG CACGAG(-5/-1)
BstEII 368 GGTCACC G^GT[ACGT]ACC
BstPI 368 GGTCACC G^GT[ACGT]ACC
CelII 1256 GCTGAGC GC^T[ACGT]AGC
DraIII 3556 CACTCAGTG CAC[ACGT][ACGT][ACGT]^GTG
EagI 21 CGGCCG C^GGCCG
EclXI 21 CGGCCG C^GGCCG
Eco52I 21 CGGCCG C^GGCCG
EcoO65I 368 GGTCACC G^GT[ACGT]ACC
EcoRV 415 GATATC GAT^ATC
HincII 1461 GTCAAC GT[CT]^[GA]AC
HindII 1461 GTCAAC GT[CT]^[GA]AC
KpnI 970 GGTACC GGTAC^C
MamI 318 GATGCCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MroI 401 TCCGGA T^CCGGA
NaeI 19 GCCGGC GCC^GGC
NgoMIV 19 GCCGGC G^CCGGC
NruI 1695 TCGCGA TCG^CGA
PinAI 1933 ACCGGT A^CCGGT
SexAI 2239 ACCTGGT A^CC[AT]GGT
SphI 13 GCATGC GCATG^C
###################################
# TWO CUT ENZYMES ( 26 enzymes) #
###################################
AatII 3087 GACGTC GACGT^C
3906 GACGTC
AccI 3090 GTCTAC GT^[AC][GT]AC
3576 GTCTAC
AcsI 2696 AAATTT [GA]^AATT[CT]
2919 AAATTT
ApoI 2696 AAATTT [GA]^AATT[CT]
2919 AAATTT
AviII 10 TGCGCA TGC^GCA
2258 TGCGCA
BbeI 111 GGCGCC GGCGC^C
3313 GGCGCC
BfaI 458 CTAG C^TAG
2379 CTAG
BsaI 1041 GAGACC GGTCTC(1/5)
2436 GAGACC
BsmBI 175 GAGACG CGTCTC(1/5)
1650 GAGACG
BsrBI 3012 GAGCGG CCGCTC(-3/-3)
3330 GAGCGG
BstXI 663 CCACCATTTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2107 CCAAGGCTGTGG
FspI 10 TGCGCA TGC^GCA
2258 TGCGCA
HgaI 177 GACGC GACGC(5/10)
1652 GACGC
HindIII 1408 AAGCTT A^AGCTT
3720 AAGCTT
KasI 111 GGCGCC G^GCGCC
3313 GGCGCC
KspI 105 CCGCGG CCGC^GG
276 CCGCGG
MaeI 458 CTAG C^TAG
2379 CTAG
MseI 850 TTAA T^TAA
1479 TTAA
NarI 111 GGCGCC GG^CGCC
3313 GGCGCC
NspI 13 GCATGC [GA]CATG^[CT]
1784 ACATGT
SacII 105 CCGCGG CCGC^GG
276 CCGCGG
SfoI 111 GGCGCC GGC^GCC
3313 GGCGCC
SmaI 1945 CCCGGG CCC^GGG
3343 CCCGGG
Tru9I 850 TTAA T^TAA
1479 TTAA
XmaCI 1945 CCCGGG C^CCGGG
3343 CCCGGG
XmaI 1945 CCCGGG C^CCGGG
3343 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 120 enzymes) #
#########################################
AccII AciI AcyI AfaI AluI
AlwI AlwNI AocI ApaI AspHI
AvaI AvaII BalI BamHI BanI
BanII BbvCI BbvI BcnI BglI
BglII BmyI BpmI BsaHI BsaJI
BsaWI BseRI BsgI BsiHKAI BsiYI
BslI BsmAI BsmFI BsoBI Bsp1286I
BspMI BsrFI BsrI BssHII BssKI
BstAPI BstF5I BstNI BstUI BstYI
Bsu36I Cac8I CfoI Cfr10I Cfr13I
DdeI DpnII DraII DrdI DsaI
EaeI EarI Eco81I EcoNI EcoO109I
EcoRII EcoT14I FauI Fnu4HI FokI
HaeII HaeIII HapII HhaI Hin1I
HinP1I HinfI HpaII HphI ItaI
Ksp632I MaeII MaeIII MboI MboII
MflI MluNI MnlI MscI MslI
MspA1I MspI MvaI MvnI MwoI
NciI NcoI NdeII NlaIII NlaIV
PflMI PleI PpuMI PstI PvuII
RsaI SacI SapI Sau3AI Sau96I
ScrFI SfaNI SfcI SmlI StuI
StyI TaqI TfiI TseI Tsp45I
Tsp509I TspRI TthHB8I Van91I XhoII