##################################
# NO CUT ENZYMES ( 48 enzymes) #
##################################
AclI AflII AgeI AscI AseI
AsnI BclI BfrI BglII BsaBI
BsiWI BspDI BspHI Bst1107I BstBI
BstZ17I ClaI EcoRV EcoT22I FbaI
HincII HindII HpaI MamI MfeI
MluI MunI NdeI NheI NotI
NruI NsiI NspV PacI PinAI
PmeI PshAI PshBI Psp1406I RcaI
SalI SfiI SfuI SnaBI SpeI
SphI SspI SwaI
###################################
# ONE CUT ENZYMES ( 28 enzymes) #
###################################
AatII 5106 GACGTC GACGT^C
Acc65I 7670 GGTACC G^GTACC
AccIII 8758 TCCGGA T^CCGGA
AhdI 2799 GACTTCCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Asp718I 7670 GGTACC G^GTACC
AspEI 2799 GACTTCCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 8331 GACGGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvrII 6962 CCTAGG C^CTAGG
BlnI 6962 CCTAGG C^CTAGG
BseAI 8758 TCCGGA T^CCGGA
Bsp1407I 5116 TGTACA T^GTACA
BspEI 8758 TCCGGA T^CCGGA
BspLU11I 4987 ACATGT A^CATGT
BsrGI 5116 TGTACA T^GTACA
BssHII 6256 GCGCGC G^CGCGC
CpoI 5143 CGGTCCG CG^G[AT]CCG
DraI 7388 TTTAAA TTT^AAA
Eam1105I 2799 GACTTCCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
KpnI 7670 GGTACC GGTAC^C
MroI 8758 TCCGGA T^CCGGA
PflFI 8331 GACGGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
PvuI 4493 CGATCG CGAT^CG
RsrII 5143 CGGTCCG CG^G[AT]CCG
ScaI 7677 AGTACT AGT^ACT
SexAI 583 ACCTGGT A^CC[AT]GGT
SgrAI 1123 CACCGGCG C[GA]^CCGG[CT]G
SspBI 5116 TGTACA T^GTACA
Tth111I 8331 GACGGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
###################################
# TWO CUT ENZYMES ( 20 enzymes) #
###################################
AcsI 2302 GAATTC [GA]^AATT[CT]
7862 GAATTC
ApoI 2302 GAATTC [GA]^AATT[CT]
7862 GAATTC
AviII 2979 TGCGCA TGC^GCA
5782 TGCGCA
BamHI 4091 GGATCC G^GATCC
4337 GGATCC
BcgI 3620 GCAGCCGCCTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
5212 GCAGCGCTGTCG
BciVI 69 GTATCC GTATCC(6/5)
1200 GTATCC
BlpI 3084 GCTCAGC GC^T[ACGT]AGC
4694 GCTCAGC
Bpu1102I 3084 GCTCAGC GC^T[ACGT]AGC
4694 GCTCAGC
BsmBI 2378 CGTCTC CGTCTC(1/5)
6208 CGTCTC
BsmI 3490 GCATTC GAATGC(1/-1)
7590 GCATTC
CelII 3084 GCTCAGC GC^T[ACGT]AGC
4694 GCTCAGC
EcoRI 2302 GAATTC G^AATTC
7862 GAATTC
FseI 4863 GGCCGGCC GGCCGG^CC
4867 GGCCGGCC
FspI 2979 TGCGCA TGC^GCA
5782 TGCGCA
HindIII 931 AAGCTT A^AGCTT
4100 AAGCTT
PaeR7I 5946 CTCGAG C^TCGAG
6895 CTCGAG
SapI 4133 GCTCTTC GCTCTTC(1/4)
7352 GCTCTTC
SbfI 2014 CCTGCAGG CCTGCA^GG
5036 CCTGCAGG
Sse8387I 2014 CCTGCAGG CCTGCA^GG
5036 CCTGCAGG
XhoI 5946 CTCGAG C^TCGAG
6895 CTCGAG
#########################################
# 3 OR MORE CUT ENZYMES ( 162 enzymes) #
#########################################
AccI AccII AciI AcyI AfaI
AflIII AluI Alw44I AlwI AlwNI
AocI Aor51HI ApaI ApaLI Asp700I
AspHI AvaI AvaII BalI BanI
BanII BbeI BbrPI BbsI BbvCI
BbvI BcnI BfaI BglI BmyI
BpmI BpuAI BsaAI BsaHI BsaI
BsaJI BsaWI BseRI BsgI BsiEI
BsiHKAI BsiYI BslI BsmAI BsmFI
BsoBI Bsp1286I BspMI BsrBI BsrDI
BsrFI BsrI BssKI BssSI BstAPI
BstEII BstF5I BstNI BstPI BstUI
BstXI BstYI Bsu36I Cac8I CfoI
Cfr10I Cfr13I DdeI DpnII DraII
DraIII DrdI DsaI EaeI EagI
EarI EclXI Eco47III Eco52I Eco81I
EcoNI EcoO109I EcoO65I EcoRII EcoT14I
FauI Fnu4HI FokI HaeII HaeIII
HapII HgaI HhaI Hin1I HinP1I
HinfI HpaII HphI ItaI KasI
Ksp632I KspI MaeI MaeII MaeIII
MboI MboII MflI MluNI MnlI
MscI MseI MslI MspA1I MspI
MvaI MvnI MwoI NaeI NarI
NciI NcoI NdeII NgoMIV NlaIII
NlaIV NspI PflMI PleI PmaCI
PmlI PpuMI PstI PvuII RsaI
SacI SacII Sau3AI Sau96I ScrFI
SfaNI SfcI SfoI SmaI SmlI
StuI StyI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI TthHB8I
Van91I XbaI XcmI XhoII XmaCI
XmaI XmnI