##################################
# NO CUT ENZYMES ( 65 enzymes) #
##################################
AatII Acc65I AclI AgeI Aor51HI
AscI AseI AsnI Asp700I Asp718I
AviII BciVI BlpI Bpu1102I BsaBI
BsiWI BsmBI BspDI BspLU11I Bst1107I
BstBI BstEII BstPI BstZ17I CelII
ClaI CpoI DrdI Eco47III EcoO65I
EcoRV EcoT22I FseI FspI HpaI
KpnI KspI MamI MfeI MluI
MunI NdeI NruI NsiI NspV
PacI PinAI PmeI PshBI Psp1406I
PvuI RsrII SacII SalI SbfI
SexAI SfiI SfuI SgrAI SnaBI
SpeI Sse8387I SwaI XbaI XmnI
###################################
# ONE CUT ENZYMES ( 35 enzymes) #
###################################
AccIII 3918 TCCGGA T^CCGGA
AflII 2737 CTTAAG C^TTAAG
AhdI 651 GACCTGGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspEI 651 GACCTGGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 4555 GACAGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
BamHI 101 GGATCC G^GATCC
BbrPI 705 CACGTG CAC^GTG
BcgI 1511 CGAGTTCAATGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI 4430 TGATCA T^GATCA
BfrI 2737 CTTAAG C^TTAAG
BglII 823 AGATCT A^GATCT
BseAI 3918 TCCGGA T^CCGGA
BspEI 3918 TCCGGA T^CCGGA
BspHI 5202 TCATGA T^CATGA
BssHII 223 GCGCGC G^CGCGC
Eam1105I 651 GACCTGGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
FbaI 4430 TGATCA T^GATCA
HincII 2655 GTTGAC GT[CT]^[GA]AC
HindII 2655 GTTGAC GT[CT]^[GA]AC
HindIII 950 AAGCTT A^AGCTT
MroI 3918 TCCGGA T^CCGGA
NaeI 3928 GCCGGC GCC^GGC
NgoMIV 3928 GCCGGC G^CCGGC
NheI 4142 GCTAGC G^CTAGC
NotI 1 GCGGCCGC GC^GGCCGC
PflFI 4555 GACAGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
PmaCI 705 CACGTG CAC^GTG
PmlI 705 CACGTG CAC^GTG
RcaI 5202 TCATGA T^CATGA
SapI 1010 GAAGAGC GCTCTTC(1/4)
ScaI 3690 AGTACT AGT^ACT
SmaI 3946 CCCGGG CCC^GGG
Tth111I 4555 GACAGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
XmaCI 3946 CCCGGG C^CCGGG
XmaI 3946 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 22 enzymes) #
###################################
BglI 1095 GCCGAGCAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
3964 GCCAGAGGGGC
BsaAI 705 CACGTG [CT]AC^GT[GA]
1956 TACGTG
BsaWI 2154 TCCGGT [AT]^CCGG[AT]
3918 TCCGGA
BsiEI 2 CGGCCG CG[GA][CT]^CG
1093 CGGCCG
BsmI 2285 GAATGC GAATGC(1/-1)
2577 GCATTC
BsrDI 550 GCAATG GCAATG(2/0)
880 GCAATG
BsrFI 2412 ACCGGC [GA]^CCGG[CT]
3928 GCCGGC
Cfr10I 2412 ACCGGC [GA]^CCGG[CT]
3928 GCCGGC
EagI 2 CGGCCG C^GGCCG
1093 CGGCCG
EarI 1010 GAAGAG CTCTTC(1/4)
3562 GAAGAG
EclXI 2 CGGCCG C^GGCCG
1093 CGGCCG
Eco52I 2 CGGCCG C^GGCCG
1093 CGGCCG
EcoNI 1885 CCTGCCCAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
2063 CCTTTGCAAGG
EcoRI 4009 GAATTC G^AATTC
5167 GAATTC
Ksp632I 1010 GAAGAG CTCTTC(1/4)
3562 GAAGAG
MslI 2293 CATATTTGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
2848 CACTCGTGTG
PflMI 1673 CCAGTCTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2378 CCATGAGATGG
PshAI 635 GACCAACGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
3526 GACGCGGGTC
SphI 2046 GCATGC GCATG^C
4511 GCATGC
SspI 94 AATATT AAT^ATT
4121 AATATT
Van91I 1673 CCAGTCTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2378 CCATGAGATGG
XcmI 3835 CCACGGGAAAGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
4221 CCAGAGCCTAGGTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 136 enzymes) #
#########################################
AccI AccII AciI AcsI AcyI
AfaI AflIII AluI Alw44I AlwI
AlwNI AocI ApaI ApaLI ApoI
AspHI AvaI AvaII AvrII BalI
BanI BanII BbeI BbsI BbvCI
BbvI BcnI BfaI BlnI BmyI
BpmI BpuAI BsaHI BsaI BsaJI
BseRI BsgI BsiHKAI BsiYI BslI
BsmAI BsmFI BsoBI Bsp1286I Bsp1407I
BspMI BsrBI BsrGI BsrI BssKI
BssSI BstAPI BstF5I BstNI BstUI
BstXI BstYI Bsu36I Cac8I CfoI
Cfr13I DdeI DpnII DraI DraII
DraIII DsaI EaeI Eco81I EcoO109I
EcoRII EcoT14I FauI Fnu4HI FokI
HaeII HaeIII HapII HgaI HhaI
Hin1I HinP1I HinfI HpaII HphI
ItaI KasI MaeI MaeII MaeIII
MboI MboII MflI MluNI MnlI
MscI MseI MspA1I MspI MvaI
MvnI MwoI NarI NciI NcoI
NdeII NlaIII NlaIV NspI PaeR7I
PleI PpuMI PstI PvuII RsaI
SacI Sau3AI Sau96I ScrFI SfaNI
SfcI SfoI SmlI SspBI StuI
StyI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I XhoI
XhoII