##################################
# NO CUT ENZYMES ( 59 enzymes) #
##################################
AatII AccI AccIII AflII AgeI
Aor51HI AscI Asp700I AspI BamHI
BbrPI BfrI BlpI Bpu1102I BseAI
BsiWI BspDI BspEI BsrDI BssHII
BssSI Bst1107I BstZ17I CelII ClaI
CpoI DraIII DrdI Eco47III EcoT22I
FseI HpaI KspI MfeI MluI
MroI MunI NheI NruI NsiI
PacI PflFI PinAI PmaCI PmeI
PmlI PvuI RsrII SacII SalI
SbfI SexAI SfiI SgrAI SnaBI
Sse8387I SwaI Tth111I XmnI
###################################
# ONE CUT ENZYMES ( 40 enzymes) #
###################################
Acc65I 1208 GGTACC G^GTACC
AclI 693 AACGTT AA^CGTT
AhdI 4942 GACTGTCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 4811 GTGCAC G^TGCAC
ApaLI 4811 GTGCAC G^TGCAC
Asp718I 1208 GGTACC G^GTACC
AspEI 4942 GACTGTCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AviII 3465 TGCGCA TGC^GCA
AvrII 3733 CCTAGG C^CTAGG
BcgI 262 GCAGTCGCCTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 4691 GTATCC GTATCC(6/5)
BglII 1650 AGATCT A^GATCT
BlnI 3733 CCTAGG C^CTAGG
BsaAI 4429 CACGTA [CT]AC^GT[GA]
BsaBI 155 GATGTTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaWI 542 ACCGGA [AT]^CCGG[AT]
BsmI 4170 GAATGC GAATGC(1/-1)
BstBI 1502 TTCGAA TT^CGAA
BstEII 4318 GGTAACC G^GT[ACGT]ACC
BstPI 4318 GGTAACC G^GT[ACGT]ACC
DraI 730 TTTAAA TTT^AAA
Eam1105I 4942 GACTGTCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoO65I 4318 GGTAACC G^GT[ACGT]ACC
EcoRV 312 GATATC GAT^ATC
FspI 3465 TGCGCA TGC^GCA
KpnI 1208 GGTACC GGTAC^C
MamI 155 GATGTTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
NotI 5157 GCGGCCGC GC^GGCCGC
NspV 1502 TTCGAA TT^CGAA
PaeR7I 4355 CTCGAG C^TCGAG
PflMI 1438 CCACTTAATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PshAI 2746 GACCTGAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
Psp1406I 693 AACGTT AA^CGTT
SacI 1427 GAGCTC GAGCT^C
SapI 1998 GAAGAGC GCTCTTC(1/4)
SfuI 1502 TTCGAA TT^CGAA
SspI 2698 AATATT AAT^ATT
Van91I 1438 CCACTTAATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XbaI 1644 TCTAGA T^CTAGA
XhoI 4355 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 27 enzymes) #
###################################
ApaI 1101 GGGCCC GGGCC^C
3155 GGGCCC
AseI 1831 ATTAAT AT^TAAT
1860 ATTAAT
AsnI 1831 ATTAAT AT^TAAT
1860 ATTAAT
BbvCI 4695 CCTCAGC CCTCAGC(-5/-2)
4756 CCTCAGC
BglI 1103 GCCCCGGCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
2887 GCCCCCAGGGC
BsiEI 1121 CGGCCG CG[GA][CT]^CG
5158 CGGCCG
BsmBI 818 CGTCTC CGTCTC(1/5)
4536 GAGACG
Bsp1407I 492 TGTACA T^GTACA
3524 TGTACA
BspHI 2930 TCATGA T^CATGA
3128 TCATGA
BspLU11I 2028 ACATGT A^CATGT
2309 ACATGT
BsrGI 492 TGTACA T^GTACA
3524 TGTACA
BstXI 1452 CCAGCAGAATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3435 CCAGAACCCTGG
EagI 1121 CGGCCG C^GGCCG
5158 CGGCCG
EclXI 1121 CGGCCG C^GGCCG
5158 CGGCCG
Eco52I 1121 CGGCCG C^GGCCG
5158 CGGCCG
NaeI 1306 GCCGGC GCC^GGC
4601 GCCGGC
NdeI 478 CATATG CA^TATG
2495 CATATG
NgoMIV 1306 GCCGGC G^CCGGC
4601 GCCGGC
PshBI 1831 ATTAAT AT^TAAT
1860 ATTAAT
RcaI 2930 TCATGA T^CATGA
3128 TCATGA
ScaI 350 AGTACT AGT^ACT
1042 AGTACT
SmaI 915 CCCGGG CCC^GGG
4014 CCCGGG
SpeI 2101 ACTAGT A^CTAGT
3835 ACTAGT
SspBI 492 TGTACA T^GTACA
3524 TGTACA
XcmI 4279 CCAGGGGACGAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
4349 CCACAACTCGAGTGG
XmaCI 915 CCCGGG C^CCGGG
4014 CCCGGG
XmaI 915 CCCGGG C^CCGGG
4014 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 132 enzymes) #
#########################################
AccII AciI AcsI AcyI AfaI
AflIII AluI AlwI AlwNI AocI
ApoI AspHI AvaI AvaII BalI
BanI BanII BbeI BbsI BbvI
BclI BcnI BfaI BmyI BpmI
BpuAI BsaHI BsaI BsaJI BseRI
BsgI BsiHKAI BsiYI BslI BsmAI
BsmFI BsoBI Bsp1286I BspMI BsrBI
BsrFI BsrI BssKI BstAPI BstF5I
BstNI BstUI BstYI Bsu36I Cac8I
CfoI Cfr10I Cfr13I DdeI DpnII
DraII DsaI EaeI EarI Eco81I
EcoNI EcoO109I EcoRI EcoRII EcoT14I
FauI FbaI Fnu4HI FokI HaeII
HaeIII HapII HgaI HhaI Hin1I
HinP1I HincII HindII HindIII HinfI
HpaII HphI ItaI KasI Ksp632I
MaeI MaeII MaeIII MboI MboII
MflI MluNI MnlI MscI MseI
MslI MspA1I MspI MvaI MvnI
MwoI NarI NciI NcoI NdeII
NlaIII NlaIV NspI PleI PpuMI
PstI PvuII RsaI Sau3AI Sau96I
ScrFI SfaNI SfcI SfoI SmlI
SphI StuI StyI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I XhoII