##################################
# NO CUT ENZYMES ( 96 enzymes) #
##################################
AatII AccII AclI AcyI AgeI
AhdI Aor51HI AscI AspEI AspI
AviII AvrII BbeI BbrPI BcgI
BciVI BlnI BlpI Bpu1102I BsaAI
BsaBI BsaHI BsaI BsiEI BsiWI
Bsp1407I BspDI BsrBI BsrGI BssHII
BssSI BstBI BstUI BstXI CelII
ClaI CpoI EagI Eam1105I EclXI
Eco47III Eco52I EcoRI EcoRV EcoT22I
FseI FspI HgaI Hin1I HpaI
KasI KspI MamI MfeI MluI
MunI MvnI NaeI NarI NgoMIV
NheI NotI NruI NsiI NspV
PacI PaeR7I PflFI PinAI PmaCI
PmeI PmlI Psp1406I PvuI RsrII
SacII SalI SapI SbfI SexAI
SfiI SfoI SfuI SgrAI SmaI
SnaBI SphI Sse8387I SspBI SspI
SwaI Tth111I XbaI XhoI XmaCI
XmaI
###################################
# ONE CUT ENZYMES ( 43 enzymes) #
###################################
AccI 2043 GTATAC GT^[AC][GT]AC
AccIII 1833 TCCGGA T^CCGGA
AflII 3092 CTTAAG C^TTAAG
AflIII 326 ACATGT A^C[GA][CT]GT
Alw44I 269 GTGCAC G^TGCAC
ApaI 741 GGGCCC GGGCC^C
ApaLI 269 GTGCAC G^TGCAC
AvaI 701 CCCGAG C^[CT]CG[GA]G
BamHI 435 GGATCC G^GATCC
BbsI 628 GAAGAC GAAGAC(2/6)
BbvCI 2712 GCTGAGG CCTCAGC(-5/-2)
BclI 978 TGATCA T^GATCA
BfrI 3092 CTTAAG C^TTAAG
BglII 2556 AGATCT A^GATCT
BpuAI 628 GAAGAC GAAGAC(2/6)
BsaWI 1833 TCCGGA [AT]^CCGG[AT]
BseAI 1833 TCCGGA T^CCGGA
BsmBI 1502 CGTCTC CGTCTC(1/5)
BsoBI 701 CCCGAG C^[CT]CG[GA]G
BspEI 1833 TCCGGA T^CCGGA
BspHI 2416 TCATGA T^CATGA
BspLU11I 326 ACATGT A^CATGT
BsrFI 507 GCCGGT [GA]^CCGG[CT]
Bst1107I 2043 GTATAC GTA^TAC
BstZ17I 2043 GTATAC GTA^TAC
CfoI 515 GCGC GCG^C
Cfr10I 507 GCCGGT [GA]^CCGG[CT]
DraIII 812 CACCGGGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 1982 GACGGGTATGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoNI 1063 CCTGCGGCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
FbaI 978 TGATCA T^GATCA
HaeII 514 GGCGCT [GA]GCGC^[CT]
HhaI 515 GCGC GCG^C
HinP1I 515 GCGC G^CGC
MroI 1833 TCCGGA T^CCGGA
NdeI 3052 CATATG CA^TATG
PshAI 1777 GACTGCAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
RcaI 2416 TCATGA T^CATGA
SacI 2166 GAGCTC GAGCT^C
ScaI 936 AGTACT AGT^ACT
SpeI 3045 ACTAGT A^CTAGT
TaqI 174 TCGA T^CGA
TthHB8I 174 TCGA T^CGA
###################################
# TWO CUT ENZYMES ( 32 enzymes) #
###################################
Acc65I 298 GGTACC G^GTACC
2512 GGTACC
AocI 440 CCTGAGG CC^T[ACGT]AGG
908 CCTGAGG
AseI 2197 ATTAAT AT^TAAT
2238 ATTAAT
AsnI 2197 ATTAAT AT^TAAT
2238 ATTAAT
Asp700I 3000 GAAGAACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
3082 GAATGGATTC
Asp718I 298 GGTACC G^GTACC
2512 GGTACC
BalI 2938 TGGCCA TGG^CCA
2955 TGGCCA
BanI 298 GGTACC G^G[CT][GA]CC
2512 GGTACC
BglI 2132 GCCCTTTGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
2582 GCCCTCAAGGC
BsgI 726 CTGCAC GTGCAG(16/14)
523 GTGCAG
BstAPI 525 GCAGCCTCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
832 GCAGAAGCTGC
BstEII 1105 GGTGACC G^GT[ACGT]ACC
2464 GGTTACC
BstPI 1105 GGTGACC G^GT[ACGT]ACC
2464 GGTTACC
Bsu36I 440 CCTGAGG CC^T[ACGT]AGG
908 CCTGAGG
DsaI 90 CCATGG C^C[GA][CT]GG
1546 CCATGG
EaeI 2938 TGGCCA [CT]^GGCC[GA]
2955 TGGCCA
Eco81I 440 CCTGAGG CC^T[ACGT]AGG
908 CCTGAGG
EcoO65I 1105 GGTGACC G^GT[ACGT]ACC
2464 GGTTACC
FauI 1110 CCCGC CCCGC(4/6)
1018 GCGGG
HincII 872 GTCAAC GT[CT]^[GA]AC
959 GTCAAC
HindII 872 GTCAAC GT[CT]^[GA]AC
959 GTCAAC
HindIII 1175 AAGCTT A^AGCTT
2631 AAGCTT
KpnI 298 GGTACC GGTAC^C
2512 GGTACC
MaeII 417 ACGT A^CGT
1501 ACGT
MluNI 2938 TGGCCA TGG^CCA
2955 TGGCCA
MscI 2938 TGGCCA TGG^CCA
2955 TGGCCA
NcoI 90 CCATGG C^CATGG
1546 CCATGG
NspI 326 ACATGT [GA]CATG^[CT]
1239 ACATGC
PshBI 2197 ATTAAT AT^TAAT
2238 ATTAAT
PvuII 310 CAGCTG CAG^CTG
1049 CAGCTG
StuI 163 AGGCCT AGG^CCT
276 AGGCCT
XmnI 3000 GAAGAACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
3082 GAATGGATTC
#########################################
# 3 OR MORE CUT ENZYMES ( 87 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
AlwNI ApoI AspHI AvaII BanII
BbvI BcnI BfaI BmyI BpmI
BsaJI BseRI BsiHKAI BsiYI BslI
BsmAI BsmFI BsmI Bsp1286I BspMI
BsrDI BsrI BssKI BstF5I BstNI
BstYI Cac8I Cfr13I DdeI DpnII
DraI DraII EarI EcoO109I EcoRII
EcoT14I Fnu4HI FokI HaeIII HapII
HinfI HpaII HphI ItaI Ksp632I
MaeI MaeIII MboI MboII MflI
MnlI MseI MslI MspA1I MspI
MvaI MwoI NciI NdeII NlaIII
NlaIV PflMI PleI PpuMI PstI
RsaI Sau3AI Sau96I ScrFI SfaNI
SfcI SmlI StyI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI Van91I
XcmI XhoII