##################################
# NO CUT ENZYMES ( 105 enzymes) #
##################################
AatII AccII AccIII AclI AcyI
AgeI AhdI Alw44I Aor51HI ApaI
ApaLI AscI AseI AsnI AspEI
AviII BalI BamHI BbeI BbrPI
BbvCI BcgI BciVI BglI BsaAI
BsaBI BsaHI BsaWI BseAI BsgI
BsiEI BsiWI BsmBI BspEI BsrBI
BssHII BssSI BstAPI BstBI BstUI
CfoI CpoI DrdI EaeI EagI
Eam1105I EclXI Eco47III Eco52I EcoNI
EcoRI FseI FspI HaeII HhaI
Hin1I HinP1I HincII HindII HpaI
KasI KspI MamI MfeI MluI
MluNI MroI MscI MslI MunI
MvnI NarI NheI NotI NruI
NspV PacI PaeR7I PinAI PmaCI
PmeI PmlI PshAI PshBI Psp1406I
PstI PvuI RsrII SacII SalI
SapI SbfI ScaI SfiI SfoI
SfuI SgrAI SmaI SnaBI Sse8387I
SwaI XcmI XhoI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 47 enzymes) #
###################################
Acc65I 176 GGTACC G^GTACC
AccI 305 GTATAC GT^[AC][GT]AC
AflII 3349 CTTAAG C^TTAAG
AocI 1401 CCTCAGG CC^T[ACGT]AGG
Asp718I 176 GGTACC G^GTACC
AspI 3089 GACTAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaI 1670 CCCGAG C^[CT]CG[GA]G
BanI 176 GGTACC G^G[CT][GA]CC
BfrI 3349 CTTAAG C^TTAAG
BlpI 1961 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 1961 GCTGAGC GC^T[ACGT]AGC
BsoBI 1670 CCCGAG C^[CT]CG[GA]G
Bsp1407I 160 TGTACA T^GTACA
BspHI 3036 TCATGA T^CATGA
BsrDI 128 CATTGC GCAATG(2/0)
BsrFI 2442 GCCGGC [GA]^CCGG[CT]
BsrGI 160 TGTACA T^GTACA
Bst1107I 305 GTATAC GTA^TAC
BstEII 1407 GGTCACC G^GT[ACGT]ACC
BstPI 1407 GGTCACC G^GT[ACGT]ACC
BstZ17I 305 GTATAC GTA^TAC
Bsu36I 1401 CCTCAGG CC^T[ACGT]AGG
CelII 1961 GCTGAGC GC^T[ACGT]AGC
Cfr10I 2442 GCCGGC [GA]^CCGG[CT]
DraIII 2519 CACAGTGTG CAC[ACGT][ACGT][ACGT]^GTG
Eco81I 1401 CCTCAGG CC^T[ACGT]AGG
EcoO65I 1407 GGTCACC G^GT[ACGT]ACC
EcoRV 1613 GATATC GAT^ATC
EcoT22I 330 ATGCAT ATGCA^T
FauI 989 CCCGC CCCGC(4/6)
HgaI 1958 GACGC GACGC(5/10)
KpnI 176 GGTACC GGTAC^C
NaeI 2442 GCCGGC GCC^GGC
NcoI 2353 CCATGG C^CATGG
NdeI 170 CATATG CA^TATG
NgoMIV 2442 GCCGGC G^CCGGC
NsiI 330 ATGCAT ATGCA^T
PflFI 3089 GACTAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 1206 CCAGCTCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PvuII 858 CAGCTG CAG^CTG
RcaI 3036 TCATGA T^CATGA
SpeI 2096 ACTAGT A^CTAGT
SphI 1744 GCATGC GCATG^C
SspBI 160 TGTACA T^GTACA
StuI 1037 AGGCCT AGG^CCT
Tth111I 3089 GACTAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 1206 CCAGCTCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
###################################
# TWO CUT ENZYMES ( 15 enzymes) #
###################################
AspHI 1388 GAGCTC G[AT]GC[AT]^C
1951 GAGCTC
AvrII 974 CCTAGG C^CTAGG
1034 CCTAGG
BlnI 974 CCTAGG C^CTAGG
1034 CCTAGG
BsiHKAI 1388 GAGCTC G[AT]GC[AT]^C
1951 GAGCTC
BspDI 1637 ATCGAT AT^CGAT
3434 ATCGAT
ClaI 1637 ATCGAT AT^CGAT
3434 ATCGAT
DsaI 1492 CCGTGG C^C[GA][CT]GG
2353 CCATGG
HindIII 1424 AAGCTT A^AGCTT
2145 AAGCTT
SacI 1388 GAGCTC GAGCT^C
1951 GAGCTC
SexAI 1570 ACCAGGT A^CC[AT]GGT
3355 ACCAGGT
SfcI 575 CTACAG C^T[GA][CT]AG
2123 CTACAG
SmlI 1931 CTCAAG C^T[CT][GA]AG
3349 CTTAAG
TaqI 1638 TCGA T^CGA
3435 TCGA
TthHB8I 1638 TCGA T^CGA
3435 TCGA
XbaI 2111 TCTAGA T^CTAGA
3012 TCTAGA
#########################################
# 3 OR MORE CUT ENZYMES ( 91 enzymes) #
#########################################
AciI AcsI AfaI AflIII AluI
AlwI AlwNI ApoI Asp700I AvaII
BanII BbsI BbvI BclI BcnI
BfaI BglII BmyI BpmI BpuAI
BsaI BsaJI BseRI BsiYI BslI
BsmAI BsmFI BsmI Bsp1286I BspLU11I
BspMI BsrI BssKI BstF5I BstNI
BstXI BstYI Cac8I Cfr13I DdeI
DpnII DraI DraII EarI EcoO109I
EcoRII EcoT14I FbaI Fnu4HI FokI
HaeIII HapII HinfI HpaII HphI
ItaI Ksp632I MaeI MaeII MaeIII
MboI MboII MflI MnlI MseI
MspA1I MspI MvaI MwoI NciI
NdeII NlaIII NlaIV NspI PleI
PpuMI RsaI Sau3AI Sau96I ScrFI
SfaNI SspI StyI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI XhoII
XmnI