##################################
# NO CUT ENZYMES ( 117 enzymes) #
##################################
AatII Acc65I AccII AccIII AclI
AcyI AflII AflIII AgeI AhdI
Alw44I AlwNI AocI Aor51HI ApaI
ApaLI AscI Asp718I AspEI AspHI
AviII AvrII BamHI BanI BbeI
BcgI BciVI BclI BfrI BlnI
BlpI Bpu1102I BsaHI BsaI BsaWI
BseAI BsgI BsiEI BsiHKAI BsiWI
BsmBI BspDI BspEI BspLU11I BspMI
BsrBI BsrFI BssHII BssSI Bst1107I
BstBI BstUI BstZ17I Bsu36I CelII
Cfr10I ClaI CpoI DrdI EagI
Eam1105I EclXI Eco47III Eco52I Eco81I
EcoRI EcoT22I FauI FbaI FseI
FspI HaeII Hin1I HindIII HpaI
KasI KpnI KspI MfeI MluI
MroI MunI MvnI NaeI NarI
NgoMIV NheI NotI NruI NsiI
NspV PacI PflMI PinAI PmeI
PshAI Psp1406I PvuI RsrII SacI
SacII SalI SbfI SexAI SfiI
SfoI SfuI SgrAI SmaI SnaBI
SpeI Sse8387I StuI SwaI Van91I
XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 33 enzymes) #
###################################
Asp700I 3642 GAATGCTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspI 2981 GACATTGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaI 514 CTCGAG C^[CT]CG[GA]G
BanII 3074 GAGCCC G[GA]GC[CT]^C
BbrPI 2213 CACGTG CAC^GTG
BglI 2291 GCCGAGATGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BmyI 3074 GAGCCC G[AGT]GC[ACT]^C
BsaAI 2213 CACGTG [CT]AC^GT[GA]
BsoBI 514 CTCGAG C^[CT]CG[GA]G
Bsp1286I 3074 GAGCCC G[AGT]GC[ACT]^C
BstAPI 908 GCAACACATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 861 GGTAACC G^GT[ACGT]ACC
BstPI 861 GGTAACC G^GT[ACGT]ACC
CfoI 69 GCGC GCG^C
DraIII 3191 CACCAAGTG CAC[ACGT][ACGT][ACGT]^GTG
EcoNI 1295 CCTTACAAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 861 GGTAACC G^GT[ACGT]ACC
HhaI 69 GCGC GCG^C
HinP1I 69 GCGC G^CGC
MslI 3534 CATTTTTGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NdeI 1656 CATATG CA^TATG
PaeR7I 514 CTCGAG C^TCGAG
PflFI 2981 GACATTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PmaCI 2213 CACGTG CAC^GTG
PmlI 2213 CACGTG CAC^GTG
SapI 285 GCTCTTC GCTCTTC(1/4)
ScaI 2034 AGTACT AGT^ACT
SphI 293 GCATGC GCATG^C
SspI 197 AATATT AAT^ATT
Tth111I 2981 GACATTGTC GAC[ACGT]^[ACGT][ACGT]GTC
XbaI 1993 TCTAGA T^CTAGA
XhoI 514 CTCGAG C^TCGAG
XmnI 3642 GAATGCTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 34 enzymes) #
###################################
AccI 1313 GTCTAC GT^[AC][GT]AC
2137 GTCTAC
AciI 3116 CCGC CCGC(-3/-1)
30 GCGG
AseI 324 ATTAAT AT^TAAT
3949 ATTAAT
AsnI 324 ATTAAT AT^TAAT
3949 ATTAAT
BalI 2092 TGGCCA TGG^CCA
2537 TGGCCA
BcnI 2879 CCGGG CC^[GC]GG
3037 CCCGG
BglII 16 AGATCT A^GATCT
3143 AGATCT
BpmI 131 CTCCAG CTGGAG(16/14)
1581 CTGGAG
BsaBI 1494 GATTTGAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
2584 GATATGGATC
BseRI 340 CTCCTC GAGGAG(10/8)
2620 GAGGAG
BsmI 3642 GAATGC GAATGC(1/-1)
3486 GCATTC
Bsp1407I 1956 TGTACA T^GTACA
3520 TGTACA
BspHI 2188 TCATGA T^CATGA
3046 TCATGA
BsrGI 1956 TGTACA T^GTACA
3520 TGTACA
DraI 3690 TTTAAA TTT^AAA
3899 TTTAAA
DsaI 847 CCATGG C^C[GA][CT]GG
997 CCATGG
EaeI 2092 TGGCCA [CT]^GGCC[GA]
2537 TGGCCA
EcoRV 1614 GATATC GAT^ATC
3926 GATATC
HapII 2879 CCGG C^CGG
3038 CCGG
HgaI 120 GACGC GACGC(5/10)
665 GCGTC
HpaII 2879 CCGG C^CGG
3038 CCGG
MamI 1494 GATTTGAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
2584 GATATGGATC
MluNI 2092 TGGCCA TGG^CCA
2537 TGGCCA
MscI 2092 TGGCCA TGG^CCA
2537 TGGCCA
MspA1I 25 CAGCTG C[AC]G^C[GT]G
1937 CAGCTG
MspI 2879 CCGG C^CGG
3038 CCGG
NciI 2879 CCGGG CC^[GC]GG
3037 CCCGG
NcoI 847 CCATGG C^CATGG
997 CCATGG
PshBI 324 ATTAAT AT^TAAT
3949 ATTAAT
PstI 1439 CTGCAG CTGCA^G
3092 CTGCAG
PvuII 25 CAGCTG CAG^CTG
1937 CAGCTG
RcaI 2188 TCATGA T^CATGA
3046 TCATGA
SspBI 1956 TGTACA T^GTACA
3520 TGTACA
XcmI 532 CCATAATAAATTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
3394 CCATAAGGCTTCTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 74 enzymes) #
#########################################
AcsI AfaI AluI AlwI ApoI
AvaII BbsI BbvCI BbvI BfaI
BpuAI BsaJI BsiYI BslI BsmAI
BsmFI BsrDI BsrI BssKI BstF5I
BstNI BstXI BstYI Cac8I Cfr13I
DdeI DpnII DraII EarI EcoO109I
EcoRII EcoT14I Fnu4HI FokI HaeIII
HincII HindII HinfI HphI ItaI
Ksp632I MaeI MaeII MaeIII MboI
MboII MflI MnlI MseI MvaI
MwoI NdeII NlaIII NlaIV NspI
PleI PpuMI RsaI Sau3AI Sau96I
ScrFI SfaNI SfcI SmlI StyI
TaqI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI TthHB8I XhoII