##################################
# NO CUT ENZYMES ( 71 enzymes) #
##################################
AatII AccII AccIII AclI AcyI
AgeI AhdI Alw44I ApaI ApaLI
AscI AspEI AvrII BanII BbeI
BbrPI BlnI BsaBI BsaHI BseAI
BsiWI BspEI BspLU11I BsrBI BssHII
BstUI CpoI DraIII DrdI Eam1105I
EcoRV FseI Hin1I KasI KspI
MamI MluI MroI MvnI NarI
NdeI NotI NruI PacI PaeR7I
PflMI PinAI PmaCI PmeI PmlI
PshAI Psp1406I PvuI RsrII SacI
SacII SalI SbfI SexAI SfiI
SfoI SgrAI SnaBI SpeI SphI
Sse8387I StuI SwaI Van91I XcmI
XhoI
###################################
# ONE CUT ENZYMES ( 52 enzymes) #
###################################
Acc65I 2331 GGTACC G^GTACC
AflII 884 CTTAAG C^TTAAG
AflIII 3684 ACGTGT A^C[GA][CT]GT
Aor51HI 2652 AGCGCT AGC^GCT
Asp700I 3765 GAAGCAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 2331 GGTACC G^GTACC
AviII 311 TGCGCA TGC^GCA
BalI 1959 TGGCCA TGG^CCA
BfrI 884 CTTAAG C^TTAAG
BsaAI 3683 TACGTG [CT]AC^GT[GA]
BsaWI 3376 TCCGGT [AT]^CCGG[AT]
BseRI 1202 CTCCTC GAGGAG(10/8)
BsiEI 2300 CGGCCG CG[GA][CT]^CG
BsmBI 1777 GAGACG CGTCTC(1/5)
BsmI 996 GAATGC GAATGC(1/-1)
Bsp1407I 4441 TGTACA T^GTACA
BspDI 4435 ATCGAT AT^CGAT
BspHI 2749 TCATGA T^CATGA
BsrGI 4441 TGTACA T^GTACA
BssSI 1986 CTCGTG CACGAG(-5/-1)
Bst1107I 2305 GTATAC GTA^TAC
BstAPI 1836 GCAAGCTCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstBI 3154 TTCGAA TT^CGAA
BstEII 4224 GGTTACC G^GT[ACGT]ACC
BstPI 4224 GGTTACC G^GT[ACGT]ACC
BstZ17I 2305 GTATAC GTA^TAC
ClaI 4435 ATCGAT AT^CGAT
EagI 2300 CGGCCG C^GGCCG
EclXI 2300 CGGCCG C^GGCCG
Eco47III 2652 AGCGCT AGC^GCT
Eco52I 2300 CGGCCG C^GGCCG
EcoNI 4148 CCTGCCCAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 4224 GGTTACC G^GT[ACGT]ACC
EcoT22I 4124 ATGCAT ATGCA^T
FspI 311 TGCGCA TGC^GCA
HaeII 2652 AGCGCT [GA]GCGC^[CT]
KpnI 2331 GGTACC GGTAC^C
MluNI 1959 TGGCCA TGG^CCA
MscI 1959 TGGCCA TGG^CCA
NaeI 351 GCCGGC GCC^GGC
NcoI 3988 CCATGG C^CATGG
NgoMIV 351 GCCGGC G^CCGGC
NheI 802 GCTAGC G^CTAGC
NsiI 4124 ATGCAT ATGCA^T
NspV 3154 TTCGAA TT^CGAA
PpuMI 4156 AGGACCT [GA]G^G[AT]CC[CT]
RcaI 2749 TCATGA T^CATGA
SapI 2672 GCTCTTC GCTCTTC(1/4)
SfuI 3154 TTCGAA TT^CGAA
SspBI 4441 TGTACA T^GTACA
XbaI 3520 TCTAGA T^CTAGA
XmnI 3765 GAAGCAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 40 enzymes) #
###################################
AocI 322 CCTGAGG CC^T[ACGT]AGG
4250 CCTCAGG
AseI 704 ATTAAT AT^TAAT
2470 ATTAAT
AsnI 704 ATTAAT AT^TAAT
2470 ATTAAT
AspI 1779 GACGGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
1817 GACGCTGTC
BamHI 2641 GGATCC G^GATCC
3716 GGATCC
BbsI 2775 GAAGAC GAAGAC(2/6)
3151 GTCTTC
BcgI 445 GCAGGACAATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
2560 GCAATATCGTCG
BciVI 4175 GGATAC GTATCC(6/5)
2403 GTATCC
BclI 1553 TGATCA T^GATCA
1629 TGATCA
BlpI 3275 GCTCAGC GC^T[ACGT]AGC
3617 GCTCAGC
Bpu1102I 3275 GCTCAGC GC^T[ACGT]AGC
3617 GCTCAGC
BpuAI 2775 GAAGAC GAAGAC(2/6)
3151 GTCTTC
BsaI 339 GAGACC GGTCTC(1/5)
1969 GGTCTC
BspMI 4716 ACCTGC ACCTGC(4/8)
923 GCAGGT
BsrDI 1281 CATTGC GCAATG(2/0)
3750 GCAATG
BstXI 2011 CCAGAGTGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
4153 CCAAGGACCTGG
Bsu36I 322 CCTGAGG CC^T[ACGT]AGG
4250 CCTCAGG
CelII 3275 GCTCAGC GC^T[ACGT]AGC
3617 GCTCAGC
DraII 2941 AGGCCCT [GA]G^G[ACGT]CC[CT]
4156 AGGACCT
DsaI 3988 CCATGG C^C[GA][CT]GG
4029 CCACGG
Eco81I 322 CCTGAGG CC^T[ACGT]AGG
4250 CCTCAGG
EcoO109I 2941 AGGCCCT [GA]G^G[ACGT]CC[CT]
4156 AGGACCT
EcoRI 3171 GAATTC G^AATTC
3456 GAATTC
FauI 408 GCGGG CCCGC(4/6)
556 GCGGG
FbaI 1553 TGATCA T^GATCA
1629 TGATCA
HgaI 1817 GACGC GACGC(5/10)
3338 GCGTC
HindIII 1038 AAGCTT A^AGCTT
1723 AAGCTT
HpaI 819 GTTAAC GTT^AAC
4462 GTTAAC
MfeI 1557 CAATTG C^AATTG
1706 CAATTG
MunI 1557 CAATTG C^AATTG
1706 CAATTG
NspI 2628 ACATGC [GA]CATG^[CT]
3544 GCATGT
PflFI 1779 GACGGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
1817 GACGCTGTC
PshBI 704 ATTAAT AT^TAAT
2470 ATTAAT
PstI 2502 CTGCAG CTGCA^G
2601 CTGCAG
ScaI 3839 AGTACT AGT^ACT
4043 AGTACT
SmaI 463 CCCGGG CCC^GGG
1849 CCCGGG
SspI 746 AATATT AAT^ATT
2228 AATATT
Tth111I 1779 GACGGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
1817 GACGCTGTC
XmaCI 463 CCCGGG C^CCGGG
1849 CCCGGG
XmaI 463 CCCGGG C^CCGGG
1849 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 95 enzymes) #
#########################################
AccI AciI AcsI AfaI AluI
AlwI AlwNI ApoI AspHI AvaI
AvaII BanI BbvCI BbvI BcnI
BfaI BglI BglII BmyI BpmI
BsaJI BsgI BsiHKAI BsiYI BslI
BsmAI BsmFI BsoBI Bsp1286I BsrFI
BsrI BssKI BstF5I BstNI BstYI
Cac8I CfoI Cfr10I Cfr13I DdeI
DpnII DraI EaeI EarI EcoRII
EcoT14I Fnu4HI FokI HaeIII HapII
HhaI HinP1I HincII HindII HinfI
HpaII HphI ItaI Ksp632I MaeI
MaeII MaeIII MboI MboII MflI
MnlI MseI MslI MspA1I MspI
MvaI MwoI NciI NdeII NlaIII
NlaIV PleI PvuII RsaI Sau3AI
Sau96I ScrFI SfaNI SfcI SmlI
StyI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I XhoII