##################################
# NO CUT ENZYMES (   78 enzymes) #
##################################

AatII       Acc65I      AccIII      AclI        AcyI        
AgeI        AhdI        Alw44I      ApaI        ApaLI       
AscI        Asp718I     AspEI       AvrII       BbeI        
BcgI        BglI        BlnI        BsaHI       BseAI       
BsiEI       BsiWI       BspDI       BspEI       BsrBI       
BsrFI       BssHII      BssSI       Bst1107I    BstZ17I     
Cfr10I      ClaI        CpoI        EagI        Eam1105I    
EclXI       Eco52I      EcoNI       EcoT22I     FseI        
Hin1I       KasI        KpnI        KspI        MluI        
MroI        NaeI        NarI        NcoI        NgoMIV      
NheI        NotI        NruI        NsiI        PacI        
PinAI       PmeI        PshAI       Psp1406I    PvuI        
RsrII       SacII       SalI        SbfI        SexAI       
SfiI        SfoI        SgrAI       SmaI        SnaBI       
SpeI        SphI        Sse8387I    SspI        SwaI        
XbaI        XmaCI       XmaI        


###################################
# ONE CUT ENZYMES (   50 enzymes) #
###################################

AccI         4229  GTAGAC               GT^[AC][GT]AC
AccII        2827  CGCG                 CG^CG
AflII        1667  CTTAAG               C^TTAAG
AocI          503  CCTCAGG              CC^T[ACGT]AGG
AseI         1118  ATTAAT               AT^TAAT
AsnI         1118  ATTAAT               AT^TAAT
AvaI            7  CTCGAG               C^[CT]CG[GA]G
AviII        3865  TGCGCA               TGC^GCA
BamHI        1889  GGATCC               G^GATCC
BbsI          290  GAAGAC               GAAGAC(2/6)
BbvCI        4238  GCTGAGG              CCTCAGC(-5/-2)
BciVI        1638  GTATCC               GTATCC(6/5)
BcnI         2740  CCGGG                CC^[GC]GG
BfrI         1667  CTTAAG               C^TTAAG
BpuAI         290  GAAGAC               GAAGAC(2/6)
BsaBI        4135  GATTTTTATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaWI        2610  ACCGGA               [AT]^CCGG[AT]
BsmBI         183  GAGACG               CGTCTC(1/5)
BsoBI           7  CTCGAG               C^[CT]CG[GA]G
BspHI        1293  TCATGA               T^CATGA
BspLU11I     4594  ACATGT               A^CATGT
BstAPI       1201  GCATTCAGTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstBI        1578  TTCGAA               TT^CGAA
BstUI        2827  CGCG                 CG^CG
BstXI        3984  CCAGCTGTATGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I        503  CCTCAGG              CC^T[ACGT]AGG
DraI         3668  TTTAAA               TTT^AAA
DrdI         4668  GACTATAATGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
DsaI         2812  CCACGG               C^C[GA][CT]GG
Eco81I        503  CCTCAGG              CC^T[ACGT]AGG
EcoRV        1409  GATATC               GAT^ATC
FspI         3865  TGCGCA               TGC^GCA
HgaI          415  GACGC                GACGC(5/10)
HpaI         3656  GTTAAC               GTT^AAC
MamI         4135  GATTTTTATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI         2596  CAATTG               C^AATTG
MunI         2596  CAATTG               C^AATTG
MvnI         2827  CGCG                 CG^CG
NciI         2740  CCGGG                CC^[GC]GG
NdeI         2340  CATATG               CA^TATG
NspV         1578  TTCGAA               TT^CGAA
PaeR7I          7  CTCGAG               C^TCGAG
PpuMI        1985  GGGTCCC              [GA]G^G[AT]CC[CT]
PshBI        1118  ATTAAT               AT^TAAT
RcaI         1293  TCATGA               T^CATGA
ScaI         4299  AGTACT               AGT^ACT
SfuI         1578  TTCGAA               TT^CGAA
StuI         3045  AGGCCT               AGG^CCT
XcmI         1146  CCACTTCCACCCTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI            7  CTCGAG               C^TCGAG


###################################
# TWO CUT ENZYMES (   35 enzymes) #
###################################

AflIII       3970  ACACGT               A^C[GA][CT]GT
             4594  ACATGT              
Aor51HI        11  AGCGCT               AGC^GCT
              334  AGCGCT              
AspI         3950  GACCCTGTC            GAC[ACGT]^[ACGT][ACGT]GTC
             3976  GACCGAGTC           
BanI         1850  GGCACC               G^G[CT][GA]CC
             3012  GGCACC              
BbrPI        3908  CACGTG               CAC^GTG
             3971  CACGTG              
BfaI          279  CTAG                 C^TAG
             1184  CTAG                
BglII         354  AGATCT               A^GATCT
             1963  AGATCT              
BlpI         3493  GCTGAGC              GC^T[ACGT]AGC
             3901  GCTCAGC             
Bpu1102I     3493  GCTGAGC              GC^T[ACGT]AGC
             3901  GCTCAGC             
BsaAI        3908  CACGTG               [CT]AC^GT[GA]
             3971  CACGTG              
Bsp1407I     4401  TGTACA               T^GTACA
             4439  TGTACA              
BsrGI        4401  TGTACA               T^GTACA
             4439  TGTACA              
BstEII        655  GGTCACC              G^GT[ACGT]ACC
              700  GGTGACC             
BstPI         655  GGTCACC              G^GT[ACGT]ACC
              700  GGTGACC             
CelII        3493  GCTGAGC              GC^T[ACGT]AGC
             3901  GCTCAGC             
DraIII       1908  CACTTGGTG            CAC[ACGT][ACGT][ACGT]^GTG
             3014  CACCATGTG           
Eco47III       11  AGCGCT               AGC^GCT
              334  AGCGCT              
EcoO65I       655  GGTCACC              G^GT[ACGT]ACC
              700  GGTGACC             
FauI         3086  CCCGC                CCCGC(4/6)
             3092  CCCGC               
HaeII          11  AGCGCT               [GA]GCGC^[CT]
              334  AGCGCT              
HapII        2611  CCGG                 C^CGG
             2740  CCGG                
HincII       1235  GTCAAC               GT[CT]^[GA]AC
             3656  GTTAAC              
HindII       1235  GTCAAC               GT[CT]^[GA]AC
             3656  GTTAAC              
HindIII      1882  AAGCTT               A^AGCTT
             3541  AAGCTT              
HpaII        2611  CCGG                 C^CGG
             2740  CCGG                
MaeI          279  CTAG                 C^TAG
             1184  CTAG                
MaeII        3909  ACGT                 A^CGT
             3972  ACGT                
MspI         2611  CCGG                 C^CGG
             2740  CCGG                
NspI         3103  GCATGT               [GA]CATG^[CT]
             4594  ACATGT              
PflFI        3950  GACCCTGTC            GAC[ACGT]^[ACGT][ACGT]GTC
             3976  GACCGAGTC           
PmaCI        3908  CACGTG               CAC^GTG
             3971  CACGTG              
PmlI         3908  CACGTG               CAC^GTG
             3971  CACGTG              
SapI         1095  GAAGAGC              GCTCTTC(1/4)
             3130  GCTCTTC             
SspBI        4401  TGTACA               T^GTACA
             4439  TGTACA              
Tth111I      3950  GACCCTGTC            GAC[ACGT]^[ACGT][ACGT]GTC
             3976  GACCGAGTC           


#########################################
# 3 OR MORE CUT ENZYMES (   95 enzymes) #
#########################################

AciI        AcsI        AfaI        AluI        AlwI        
AlwNI       ApoI        Asp700I     AspHI       AvaII       
BalI        BanII       BbvI        BclI        BmyI        
BpmI        BsaI        BsaJI       BseRI       BsgI        
BsiHKAI     BsiYI       BslI        BsmAI       BsmFI       
BsmI        Bsp1286I    BspMI       BsrDI       BsrI        
BssKI       BstF5I      BstNI       BstYI       Cac8I       
CfoI        Cfr13I      DdeI        DpnII       DraII       
EaeI        EarI        EcoO109I    EcoRI       EcoRII      
EcoT14I     FbaI        Fnu4HI      FokI        HaeIII      
HhaI        HinP1I      HinfI       HphI        ItaI        
Ksp632I     MaeIII      MboI        MboII       MflI        
MluNI       MnlI        MscI        MseI        MslI        
MspA1I      MvaI        MwoI        NdeII       NlaIII      
NlaIV       PflMI       PleI        PstI        PvuII       
RsaI        SacI        Sau3AI      Sau96I      ScrFI       
SfaNI       SfcI        SmlI        StyI        TaqI        
TfiI        Tru9I       TseI        Tsp45I      Tsp509I     
TspRI       TthHB8I     Van91I      XhoII       XmnI