##################################
# NO CUT ENZYMES ( 101 enzymes) #
##################################
AatII Acc65I AccIII AclI AcyI
AflII AflIII AgeI AhdI Alw44I
Aor51HI ApaLI AscI AseI AsnI
Asp700I Asp718I AspEI AspI AvrII
BamHI BbeI BbrPI BciVI BclI
BfrI BglII BlnI BsaAI BsaBI
BsaHI BseAI BsiEI BsiWI Bsp1407I
BspDI BspEI BspLU11I BsrGI BssHII
BstBI ClaI CpoI DraI EagI
Eam1105I EclXI Eco47III Eco52I EcoRI
EcoRV EcoT22I FbaI FseI HaeII
HgaI Hin1I HindIII HpaI KasI
KpnI KspI MamI MluI MroI
NarI NdeI NheI NotI NruI
NsiI NspV PacI PflFI PflMI
PinAI PmaCI PmeI PmlI PshAI
PshBI Psp1406I PvuI RsrII SacII
SalI SbfI ScaI SfoI SfuI
SgrAI SnaBI SpeI Sse8387I SspBI
SspI SwaI Tth111I Van91I XbaI
XmnI
###################################
# ONE CUT ENZYMES ( 29 enzymes) #
###################################
AccII 1017 CGCG CG^CG
AcsI 3061 GAATTT [GA]^AATT[CT]
ApoI 3061 GAATTT [GA]^AATT[CT]
AviII 470 TGCGCA TGC^GCA
BsaWI 166 ACCGGA [AT]^CCGG[AT]
BsmBI 2921 GAGACG CGTCTC(1/5)
BsmI 3030 GAATGC GAATGC(1/-1)
BspHI 1163 TCATGA T^CATGA
Bst1107I 317 GTATAC GTA^TAC
BstEII 2146 GGTCACC G^GT[ACGT]ACC
BstPI 2146 GGTCACC G^GT[ACGT]ACC
BstUI 1017 CGCG CG^CG
BstZ17I 317 GTATAC GTA^TAC
CfoI 471 GCGC GCG^C
DraIII 1937 CACGTCGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 1951 GACCGCTGTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoO65I 2146 GGTCACC G^GT[ACGT]ACC
FspI 470 TGCGCA TGC^GCA
HhaI 471 GCGC GCG^C
HinP1I 471 GCGC G^CGC
MfeI 2899 CAATTG C^AATTG
MunI 2899 CAATTG C^AATTG
MvnI 1017 CGCG CG^CG
NaeI 1575 GCCGGC GCC^GGC
NgoMIV 1575 GCCGGC G^CCGGC
RcaI 1163 TCATGA T^CATGA
SacI 2293 GAGCTC GAGCT^C
SexAI 1975 ACCTGGT A^CC[AT]GGT
SfiI 2565 GGCCCAGCTGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
###################################
# TWO CUT ENZYMES ( 23 enzymes) #
###################################
AccI 317 GTATAC GT^[AC][GT]AC
3037 GTCTAC
AocI 2059 CCTGAGG CC^T[ACGT]AGG
2191 CCTGAGG
BalI 90 TGGCCA TGG^CCA
1046 TGGCCA
BsaI 364 GAGACC GGTCTC(1/5)
1464 GGTCTC
BsrFI 1394 ACCGGC [GA]^CCGG[CT]
1575 GCCGGC
BssSI 568 CACGAG CACGAG(-5/-1)
1694 CACGAG
BstAPI 1113 GCACTGTCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
2788 GCAGGACCTGC
BstXI 2163 CCAGCTCAATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2179 CCAGGATCATGG
BstYI 573 GGATCT [GA]^GATC[CT]
3139 GGATCT
Bsu36I 2059 CCTGAGG CC^T[ACGT]AGG
2191 CCTGAGG
Cfr10I 1394 ACCGGC [GA]^CCGG[CT]
1575 GCCGGC
Eco81I 2059 CCTGAGG CC^T[ACGT]AGG
2191 CCTGAGG
MflI 573 GGATCT [GA]^GATC[CT]
3139 GGATCT
MluNI 90 TGGCCA TGG^CCA
1046 TGGCCA
MscI 90 TGGCCA TGG^CCA
1046 TGGCCA
MseI 3180 TTAA T^TAA
3279 TTAA
PaeR7I 844 CTCGAG C^TCGAG
2509 CTCGAG
SphI 1680 GCATGC GCATG^C
2862 GCATGC
StuI 230 AGGCCT AGG^CCT
2381 AGGCCT
Tru9I 3180 TTAA T^TAA
3279 TTAA
XcmI 1099 CCAGGGGATGATTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
2466 CCAGCCCTTCACTGG
XhoI 844 CTCGAG C^TCGAG
2509 CTCGAG
XhoII 573 GGATCT [GA]^GATC[CT]
3139 GGATCT
#########################################
# 3 OR MORE CUT ENZYMES ( 105 enzymes) #
#########################################
AciI AfaI AluI AlwI AlwNI
ApaI AspHI AvaI AvaII BanI
BanII BbsI BbvCI BbvI BcgI
BcnI BfaI BglI BlpI BmyI
BpmI Bpu1102I BpuAI BsaJI BseRI
BsgI BsiHKAI BsiYI BslI BsmAI
BsmFI BsoBI Bsp1286I BspMI BsrBI
BsrDI BsrI BssKI BstF5I BstNI
Cac8I CelII Cfr13I DdeI DpnII
DraII DsaI EaeI EarI EcoNI
EcoO109I EcoRII EcoT14I FauI Fnu4HI
FokI HaeIII HapII HincII HindII
HinfI HpaII HphI ItaI Ksp632I
MaeI MaeII MaeIII MboI MboII
MnlI MslI MspA1I MspI MvaI
MwoI NciI NcoI NdeII NlaIII
NlaIV NspI PleI PpuMI PstI
PvuII RsaI SapI Sau3AI Sau96I
ScrFI SfaNI SfcI SmaI SmlI
StyI TaqI TfiI TseI Tsp45I
Tsp509I TspRI TthHB8I XmaCI XmaI