##################################
# NO CUT ENZYMES ( 61 enzymes) #
##################################
AatII Acc65I AcyI AflII AgeI
Alw44I Aor51HI ApaLI AscI Asp718I
AspI AvrII BbeI BcgI BciVI
BfrI BglI BglII BlnI BsaHI
BsiWI BspDI BssHII BssSI Bst1107I
BstZ17I ClaI CpoI DraIII Eco47III
FseI HaeII Hin1I KasI KpnI
KspI MluI NarI NheI NotI
NruI PacI PflFI PinAI PmeI
PvuI RsrII SacII SalI SbfI
SfiI SfoI SgrAI SmaI SnaBI
Sse8387I SwaI Tth111I XbaI XmaCI
XmaI
###################################
# ONE CUT ENZYMES ( 54 enzymes) #
###################################
AccII 3599 CGCG CG^CG
AccIII 1473 TCCGGA T^CCGGA
AclI 3039 AACGTT AA^CGTT
AhdI 2140 GACAAGGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AocI 5632 CCTGAGG CC^T[ACGT]AGG
ApaI 2389 GGGCCC GGGCC^C
AseI 3973 ATTAAT AT^TAAT
AsnI 3973 ATTAAT AT^TAAT
Asp700I 3081 GAAATGTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspEI 2140 GACAAGGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AviII 6066 TGCGCA TGC^GCA
BanI 4728 GGTGCC G^G[CT][GA]CC
BbrPI 1940 CACGTG CAC^GTG
BbvCI 5134 CCTCAGC CCTCAGC(-5/-2)
BseAI 1473 TCCGGA T^CCGGA
BsgI 4916 CTGCAC GTGCAG(16/14)
BsiEI 3417 CGGCCG CG[GA][CT]^CG
BsmBI 1246 CGTCTC CGTCTC(1/5)
BspEI 1473 TCCGGA T^CCGGA
BsrBI 4318 GAGCGG CCGCTC(-3/-3)
BsrFI 4830 GCCGGC [GA]^CCGG[CT]
BstAPI 4467 GCAACAGATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstBI 2766 TTCGAA TT^CGAA
BstUI 3599 CGCG CG^CG
Bsu36I 5632 CCTGAGG CC^T[ACGT]AGG
Cfr10I 4830 GCCGGC [GA]^CCGG[CT]
DrdI 4949 GACAGACATGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EagI 3417 CGGCCG C^GGCCG
Eam1105I 2140 GACAAGGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EclXI 3417 CGGCCG C^GGCCG
Eco52I 3417 CGGCCG C^GGCCG
Eco81I 5632 CCTGAGG CC^T[ACGT]AGG
EcoRV 2242 GATATC GAT^ATC
FspI 6066 TGCGCA TGC^GCA
HgaI 3922 GACGC GACGC(5/10)
HindIII 3436 AAGCTT A^AGCTT
HpaI 3036 GTTAAC GTT^AAC
MroI 1473 TCCGGA T^CCGGA
MvnI 3599 CGCG CG^CG
NaeI 4830 GCCGGC GCC^GGC
NdeI 5381 CATATG CA^TATG
NgoMIV 4830 GCCGGC G^CCGGC
NspV 2766 TTCGAA TT^CGAA
PflMI 4767 CCAGTATGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PmaCI 1940 CACGTG CAC^GTG
PmlI 1940 CACGTG CAC^GTG
PshAI 3546 GACTGCTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PshBI 3973 ATTAAT AT^TAAT
Psp1406I 3039 AACGTT AA^CGTT
PstI 5881 CTGCAG CTGCA^G
SfuI 2766 TTCGAA TT^CGAA
SpeI 628 ACTAGT A^CTAGT
Van91I 4767 CCAGTATGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XmnI 3081 GAAATGTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 29 enzymes) #
###################################
AvaI 2825 CTCGAG C^[CT]CG[GA]G
5246 CTCGAG
BsaAI 1860 CACGTA [CT]AC^GT[GA]
1940 CACGTG
BsaBI 658 GATTTATATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
3775 GATCTTGATC
BsaWI 1473 TCCGGA [AT]^CCGG[AT]
5519 ACCGGA
BsmI 2540 GAATGC GAATGC(1/-1)
3856 GAATGC
BsoBI 2825 CTCGAG C^[CT]CG[GA]G
5246 CTCGAG
BspMI 256 ACCTGC ACCTGC(4/8)
4206 ACCTGC
BsrDI 1958 GCAATG GCAATG(2/0)
5648 GCAATG
CfoI 6053 GCGC GCG^C
6067 GCGC
DsaI 1076 CCATGG C^C[GA][CT]GG
1289 CCATGG
EcoNI 862 CCTGATTCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
4107 CCTAAAGGAGG
EcoT22I 192 ATGCAT ATGCA^T
1103 ATGCAT
FauI 4530 CCCGC CCCGC(4/6)
4320 GCGGG
HhaI 6053 GCGC GCG^C
6067 GCGC
HinP1I 6053 GCGC G^CGC
6067 GCGC
HincII 3036 GTTAAC GT[CT]^[GA]AC
5889 GTCAAC
HindII 3036 GTTAAC GT[CT]^[GA]AC
5889 GTCAAC
MamI 658 GATTTATATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
3775 GATCTTGATC
MfeI 3102 CAATTG C^AATTG
5006 CAATTG
MspA1I 2940 CAGCTG C[AC]G^C[GT]G
3847 CAGCTG
MunI 3102 CAATTG C^AATTG
5006 CAATTG
NcoI 1076 CCATGG C^CATGG
1289 CCATGG
NsiI 192 ATGCAT ATGCA^T
1103 ATGCAT
PaeR7I 2825 CTCGAG C^TCGAG
5246 CTCGAG
PpuMI 2417 GGGACCC [GA]G^G[AT]CC[CT]
5636 AGGACCT
PvuII 2940 CAGCTG CAG^CTG
3847 CAGCTG
SexAI 3251 ACCAGGT A^CC[AT]GGT
4557 ACCAGGT
StuI 4574 AGGCCT AGG^CCT
5402 AGGCCT
XhoI 2825 CTCGAG C^TCGAG
5246 CTCGAG
#########################################
# 3 OR MORE CUT ENZYMES ( 114 enzymes) #
#########################################
AccI AciI AcsI AfaI AflIII
AluI AlwI AlwNI ApoI AspHI
AvaII BalI BamHI BanII BbsI
BbvI BclI BcnI BfaI BlpI
BmyI BpmI Bpu1102I BpuAI BsaI
BsaJI BseRI BsiHKAI BsiYI BslI
BsmAI BsmFI Bsp1286I Bsp1407I BspHI
BspLU11I BsrGI BsrI BssKI BstEII
BstF5I BstNI BstPI BstXI BstYI
Cac8I CelII Cfr13I DdeI DpnII
DraI DraII EaeI EarI EcoO109I
EcoO65I EcoRI EcoRII EcoT14I FbaI
Fnu4HI FokI HaeIII HapII HinfI
HpaII HphI ItaI Ksp632I MaeI
MaeII MaeIII MboI MboII MflI
MluNI MnlI MscI MseI MslI
MspI MvaI MwoI NciI NdeII
NlaIII NlaIV NspI PleI RcaI
RsaI SacI SapI Sau3AI Sau96I
ScaI ScrFI SfaNI SfcI SmlI
SphI SspBI SspI StyI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I XcmI XhoII