##################################
# NO CUT ENZYMES ( 72 enzymes) #
##################################
AatII AccII AccIII AclI AflII
Aor51HI ApaI AscI AviII BanII
BbsI BcgI BfrI BpuAI BsaI
BseAI BsiWI BsmBI Bsp1407I BspDI
BspEI BsrBI BsrGI BssHII BstAPI
BstBI BstEII BstPI BstUI ClaI
CpoI DsaI EagI EclXI Eco47III
Eco52I EcoO65I EcoRV FseI FspI
KspI MluI MroI MvnI NaeI
NcoI NgoMIV NheI NotI NruI
NspV PacI PaeR7I PmeI Psp1406I
PvuI RsrII SacI SacII SapI
SbfI ScaI SfiI SfuI SgrAI
SmaI SphI Sse8387I SspBI XhoI
XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 36 enzymes) #
###################################
Acc65I 1587 GGTACC G^GTACC
AgeI 1158 ACCGGT A^CCGGT
Asp718I 1587 GGTACC G^GTACC
AspI 5186 GACCGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaI 1717 CCCGAG C^[CT]CG[GA]G
BamHI 1686 GGATCC G^GATCC
BbeI 1370 GGCGCC GGCGC^C
BbvCI 1926 GCTGAGG CCTCAGC(-5/-2)
BlpI 6837 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 6837 GCTGAGC GC^T[ACGT]AGC
BsaWI 1158 ACCGGT [AT]^CCGG[AT]
BsiEI 5185 CGACCG CG[GA][CT]^CG
BsoBI 1717 CCCGAG C^[CT]CG[GA]G
BspHI 1794 TCATGA T^CATGA
BspMI 1350 ACCTGC ACCTGC(4/8)
BssSI 1459 CACGAG CACGAG(-5/-1)
CelII 6837 GCTGAGC GC^T[ACGT]AGC
CfoI 1371 GCGC GCG^C
EcoNI 5922 CCTAGGGCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
FauI 1530 CCCGC CCCGC(4/6)
HaeII 1370 GGCGCC [GA]GCGC^[CT]
HhaI 1371 GCGC GCG^C
HinP1I 1371 GCGC G^CGC
HpaI 7032 GTTAAC GTT^AAC
KasI 1370 GGCGCC G^GCGCC
KpnI 1587 GGTACC GGTAC^C
NarI 1370 GGCGCC GG^CGCC
PflFI 5186 GACCGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
PinAI 1158 ACCGGT A^CCGGT
PpuMI 1445 AGGTCCT [GA]G^G[AT]CC[CT]
RcaI 1794 TCATGA T^CATGA
SalI 977 GTCGAC G^TCGAC
SfoI 1370 GGCGCC GGC^GCC
SnaBI 150 TACGTA TAC^GTA
StuI 1540 AGGCCT AGG^CCT
Tth111I 5186 GACCGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
###################################
# TWO CUT ENZYMES ( 33 enzymes) #
###################################
AhdI 4296 GACTATTTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
4451 GACAGTTTGTC
Alw44I 5672 GTGCAC G^TGCAC
7421 GTGCAC
AlwNI 549 CAGTGGCTG CAG[ACGT][ACGT][ACGT]^CTG
5731 CAGATTCTG
AocI 872 CCTCAGG CC^T[ACGT]AGG
2907 CCTGAGG
ApaLI 5672 GTGCAC G^TGCAC
7421 GTGCAC
AspEI 4296 GACTATTTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
4451 GACAGTTTGTC
AvrII 1438 CCTAGG C^CTAGG
5922 CCTAGG
BbrPI 5712 CACGTG CAC^GTG
7455 CACGTG
BclI 4594 TGATCA T^GATCA
6798 TGATCA
BlnI 1438 CCTAGG C^CTAGG
5922 CCTAGG
BsrDI 4306 CATTGC GCAATG(2/0)
840 GCAATG
Bst1107I 3789 GTATAC GTA^TAC
5807 GTATAC
BstZ17I 3789 GTATAC GTA^TAC
5807 GTATAC
Bsu36I 872 CCTCAGG CC^T[ACGT]AGG
2907 CCTGAGG
DraII 1445 AGGTCCT [GA]G^G[ACGT]CC[CT]
3054 GGGGCCT
DraIII 2035 CACATGGTG CAC[ACGT][ACGT][ACGT]^GTG
3760 CACTGTGTG
DrdI 968 GACTCACCTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
3252 GACTGTTGCGTC
Eam1105I 4296 GACTATTTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
4451 GACAGTTTGTC
Eco81I 872 CCTCAGG CC^T[ACGT]AGG
2907 CCTGAGG
EcoO109I 1445 AGGTCCT [GA]G^G[ACGT]CC[CT]
3054 GGGGCCT
FbaI 4594 TGATCA T^GATCA
6798 TGATCA
MfeI 2247 CAATTG C^AATTG
4834 CAATTG
MunI 2247 CAATTG C^AATTG
4834 CAATTG
PmaCI 5712 CACGTG CAC^GTG
7455 CACGTG
PmlI 5712 CACGTG CAC^GTG
7455 CACGTG
PshAI 712 GACACAAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
3113 GACTAAGGTC
SexAI 767 ACCAGGT A^CC[AT]GGT
7462 ACCAGGT
SmlI 5534 CTCAAG C^T[CT][GA]AG
6143 CTCAAG
SpeI 6653 ACTAGT A^CTAGT
7610 ACTAGT
SspI 510 AATATT AAT^ATT
7408 AATATT
SwaI 3513 ATTTAAAT ATTT^AAAT
4230 ATTTAAAT
XbaI 575 TCTAGA T^CTAGA
3546 TCTAGA
XcmI 750 CCAAAAACAAATTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
3934 CCAGAAATAATATGG
#########################################
# 3 OR MORE CUT ENZYMES ( 117 enzymes) #
#########################################
AccI AciI AcsI AcyI AfaI
AflIII AluI AlwI ApoI AseI
AsnI Asp700I AspHI AvaII BalI
BanI BbvI BciVI BcnI BfaI
BglI BglII BmyI BpmI BsaAI
BsaBI BsaHI BsaJI BseRI BsgI
BsiHKAI BsiYI BslI BsmAI BsmFI
BsmI Bsp1286I BspLU11I BsrFI BsrI
BssKI BstF5I BstNI BstXI BstYI
Cac8I Cfr10I Cfr13I DdeI DpnII
DraI EaeI EarI EcoRI EcoRII
EcoT14I EcoT22I Fnu4HI FokI HaeIII
HapII HgaI Hin1I HincII HindII
HindIII HinfI HpaII HphI ItaI
Ksp632I MaeI MaeII MaeIII MamI
MboI MboII MflI MluNI MnlI
MscI MseI MslI MspA1I MspI
MvaI MwoI NciI NdeI NdeII
NlaIII NlaIV NsiI NspI PflMI
PleI PshBI PstI PvuII RsaI
Sau3AI Sau96I ScrFI SfaNI SfcI
StyI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I Van91I
XhoII XmnI