##################################
# NO CUT ENZYMES ( 82 enzymes) #
##################################
AatII Acc65I AccII AclI AgeI
AhdI Aor51HI AscI Asp718I AspEI
AspI AviII BalI BanI BbeI
BcgI BglI BlpI Bpu1102I BsiWI
BsmBI BspDI BsrBI BsrFI BssHII
BssSI BstBI BstEII BstPI BstUI
CelII Cfr10I ClaI CpoI DrdI
DsaI Eam1105I Eco47III EcoO65I EcoRV
FseI FspI HaeII KasI KpnI
KspI MluI MluNI MscI MvnI
NaeI NarI NcoI NgoMIV NotI
NruI NspV PacI PaeR7I PflFI
PinAI PmeI PshAI Psp1406I PvuI
RsrII SacI SacII SalI SapI
SbfI ScaI SfiI SfoI SfuI
SgrAI SnaBI Sse8387I StuI SwaI
Tth111I XhoI
###################################
# ONE CUT ENZYMES ( 57 enzymes) #
###################################
AccI 5215 GTATAC GT^[AC][GT]AC
AccIII 574 TCCGGA T^CCGGA
AcyI 59 GACGCC G[GA]^CG[CT]C
AflII 4322 CTTAAG C^TTAAG
Alw44I 5370 GTGCAC G^TGCAC
ApaI 709 GGGCCC GGGCC^C
ApaLI 5370 GTGCAC G^TGCAC
AvrII 4370 CCTAGG C^CTAGG
BamHI 830 GGATCC G^GATCC
BanII 709 GGGCCC G[GA]GC[CT]^C
BbrPI 1452 CACGTG CAC^GTG
BbvCI 5478 GCTGAGG CCTCAGC(-5/-2)
BciVI 3585 GTATCC GTATCC(6/5)
BclI 408 TGATCA T^GATCA
BfrI 4322 CTTAAG C^TTAAG
BglII 4423 AGATCT A^GATCT
BlnI 4370 CCTAGG C^CTAGG
BsaAI 1452 CACGTG [CT]AC^GT[GA]
BsaHI 59 GACGCC G[GA]^CG[CT]C
BsaI 5513 GAGACC GGTCTC(1/5)
BseAI 574 TCCGGA T^CCGGA
BsgI 2171 CTGCAC GTGCAG(16/14)
BsiEI 38 CGGCCG CG[GA][CT]^CG
Bsp1407I 1656 TGTACA T^GTACA
BspEI 574 TCCGGA T^CCGGA
BspMI 1129 GCAGGT ACCTGC(4/8)
BsrGI 1656 TGTACA T^GTACA
Bst1107I 5215 GTATAC GTA^TAC
BstXI 5463 CCAGCAAGTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstZ17I 5215 GTATAC GTA^TAC
DraIII 4012 CACAATGTG CAC[ACGT][ACGT][ACGT]^GTG
EaeI 38 CGGCCG [CT]^GGCC[GA]
EagI 38 CGGCCG C^GGCCG
EclXI 38 CGGCCG C^GGCCG
Eco52I 38 CGGCCG C^GGCCG
EcoNI 5612 CCTGAGGCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoT14I 4370 CCTAGG C^C[AT][AT]GG
EcoT22I 4130 ATGCAT ATGCA^T
FbaI 408 TGATCA T^GATCA
HgaI 59 GACGC GACGC(5/10)
Hin1I 59 GACGCC G[GA]^CG[CT]C
HindIII 3603 AAGCTT A^AGCTT
MroI 574 TCCGGA T^CCGGA
NheI 4952 GCTAGC G^CTAGC
NsiI 4130 ATGCAT ATGCA^T
PmaCI 1452 CACGTG CAC^GTG
PmlI 1452 CACGTG CAC^GTG
PpuMI 4925 GGGACCT [GA]G^G[AT]CC[CT]
SexAI 5219 ACCTGGT A^CC[AT]GGT
SmaI 63 CCCGGG CCC^GGG
SpeI 3680 ACTAGT A^CTAGT
SphI 5583 GCATGC GCATG^C
SspBI 1656 TGTACA T^GTACA
StyI 4370 CCTAGG C^C[AT][AT]GG
XbaI 205 TCTAGA T^CTAGA
XmaCI 63 CCCGGG C^CCGGG
XmaI 63 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 28 enzymes) #
###################################
AflIII 3210 ACATGT A^C[GA][CT]GT
3566 ACATGT
AlwNI 846 CAGTGCCTG CAG[ACGT][ACGT][ACGT]^CTG
1377 CAGTTCCTG
AocI 1088 CCTCAGG CC^T[ACGT]AGG
5612 CCTGAGG
Asp700I 675 GAAACCTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2162 GAAATATTTC
AspHI 2241 GTGCTC G[AT]GC[AT]^C
5370 GTGCAC
AvaI 63 CCCGGG C^[CT]CG[GA]G
903 CCCGAG
BsaBI 118 GATATTTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
4788 GATTTTTATC
BsaWI 574 TCCGGA [AT]^CCGG[AT]
2928 TCCGGT
BsiHKAI 2241 GTGCTC G[AT]GC[AT]^C
5370 GTGCAC
BsoBI 63 CCCGGG C^[CT]CG[GA]G
903 CCCGAG
BspHI 5081 TCATGA T^CATGA
5098 TCATGA
BspLU11I 3210 ACATGT A^CATGT
3566 ACATGT
Bsu36I 1088 CCTCAGG CC^T[ACGT]AGG
5612 CCTGAGG
CfoI 76 GCGC GCG^C
5669 GCGC
Eco81I 1088 CCTCAGG CC^T[ACGT]AGG
5612 CCTGAGG
HhaI 76 GCGC GCG^C
5669 GCGC
HinP1I 76 GCGC G^CGC
5669 GCGC
HpaI 2449 GTTAAC GTT^AAC
2911 GTTAAC
MamI 118 GATATTTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
4788 GATTTTTATC
MfeI 110 CAATTG C^AATTG
3749 CAATTG
MspA1I 90 CAGCTG C[AC]G^C[GT]G
621 CAGCTG
MunI 110 CAATTG C^AATTG
3749 CAATTG
PflMI 608 CCATCTGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
4875 CCACAGACTGG
PvuII 90 CAGCTG CAG^CTG
621 CAGCTG
RcaI 5081 TCATGA T^CATGA
5098 TCATGA
Van91I 608 CCATCTGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
4875 CCACAGACTGG
XcmI 404 CCATTGATCATCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
1955 CCACATAGGCACTGG
XmnI 675 GAAACCTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2162 GAAATATTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 91 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
ApoI AseI AsnI AvaII BbsI
BbvI BcnI BfaI BmyI BpmI
BpuAI BsaJI BseRI BsiYI BslI
BsmAI BsmFI BsmI Bsp1286I BsrDI
BsrI BssKI BstAPI BstF5I BstNI
BstYI Cac8I Cfr13I DdeI DpnII
DraI DraII EarI EcoO109I EcoRI
EcoRII FauI Fnu4HI FokI HaeIII
HapII HincII HindII HinfI HpaII
HphI ItaI Ksp632I MaeI MaeII
MaeIII MboI MboII MflI MnlI
MseI MslI MspI MvaI MwoI
NciI NdeI NdeII NlaIII NlaIV
NspI PleI PshBI PstI RsaI
Sau3AI Sau96I ScrFI SfaNI SfcI
SmlI SspI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI TthHB8I
XhoII