##################################
# NO CUT ENZYMES ( 88 enzymes) #
##################################
Acc65I AccII AccIII AhdI Aor51HI
ApaI AscI Asp718I AspEI AviII
BamHI BbeI BbrPI BcgI BcnI
BglII BlpI Bpu1102I BseAI BsiEI
BsiWI BsmBI BspDI BspEI BspMI
BsrBI BssHII BstBI BstEII BstPI
BstUI CelII ClaI CpoI DraIII
DsaI EagI Eam1105I EclXI Eco47III
Eco52I EcoO65I FseI FspI HaeII
KasI KpnI KspI MluI MroI
MvnI NaeI NarI NciI NcoI
NdeI NgoMIV NheI NotI NruI
NspV PacI PaeR7I PflMI PmaCI
PmeI PmlI PvuI RsrII SacII
SalI SbfI SexAI SfiI SfoI
SfuI SgrAI SmaI SnaBI SpeI
SphI Sse8387I StuI SwaI Van91I
XhoI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 60 enzymes) #
###################################
AatII 1964 GACGTC GACGT^C
AclI 3138 AACGTT AA^CGTT
AflII 3127 CTTAAG C^TTAAG
AgeI 3548 ACCGGT A^CCGGT
Alw44I 3022 GTGCAC G^TGCAC
AocI 3082 CCTCAGG CC^T[ACGT]AGG
ApaLI 3022 GTGCAC G^TGCAC
AspI 2009 GACTGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaI 531 CTCGGG C^[CT]CG[GA]G
AvrII 1364 CCTAGG C^CTAGG
BanII 1536 GAGCTC G[GA]GC[CT]^C
BfrI 3127 CTTAAG C^TTAAG
BlnI 1364 CCTAGG C^CTAGG
BpmI 2327 CTCCAG CTGGAG(16/14)
BsaAI 1119 CACGTA [CT]AC^GT[GA]
BsaBI 2633 GATTTGAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaI 3853 GGTCTC GGTCTC(1/5)
BsaWI 3548 ACCGGT [AT]^CCGG[AT]
BsgI 1678 GTGCAG GTGCAG(16/14)
BsoBI 531 CTCGGG C^[CT]CG[GA]G
Bsp1407I 775 TGTACA T^GTACA
BsrDI 562 CATTGC GCAATG(2/0)
BsrFI 3548 ACCGGT [GA]^CCGG[CT]
BsrGI 775 TGTACA T^GTACA
BssSI 1848 CTCGTG CACGAG(-5/-1)
Bst1107I 1007 GTATAC GTA^TAC
BstAPI 1680 GCAGCTTATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstXI 817 CCAAGGTCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstZ17I 1007 GTATAC GTA^TAC
Bsu36I 3082 CCTCAGG CC^T[ACGT]AGG
Cfr10I 3548 ACCGGT [GA]^CCGG[CT]
DrdI 1958 GACCTTGACGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I 3082 CCTCAGG CC^T[ACGT]AGG
EcoNI 140 CCTGAAGAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRI 1631 GAATTC G^AATTC
EcoRV 2141 GATATC GAT^ATC
FauI 3049 GCGGG CCCGC(4/6)
HapII 3549 CCGG C^CGG
HgaI 1454 GACGC GACGC(5/10)
HindIII 119 AAGCTT A^AGCTT
HpaII 3549 CCGG C^CGG
MaeIII 1956 GTGAC ^GT[ACGT]AC
MamI 2633 GATTTGAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI 2100 CAATTG C^AATTG
MspI 3549 CCGG C^CGG
MunI 2100 CAATTG C^AATTG
PflFI 2009 GACTGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PinAI 3548 ACCGGT A^CCGGT
PshAI 390 GACAGGTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
Psp1406I 3138 AACGTT AA^CGTT
PstI 330 CTGCAG CTGCA^G
SacI 1536 GAGCTC GAGCT^C
ScaI 3983 AGTACT AGT^ACT
SfcI 330 CTGCAG C^T[GA][CT]AG
SspBI 775 TGTACA T^GTACA
TaqI 2827 TCGA T^CGA
Tsp45I 1956 GTGAC ^GT[GC]AC
Tth111I 2009 GACTGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
TthHB8I 2827 TCGA T^CGA
XbaI 2276 TCTAGA T^CTAGA
###################################
# TWO CUT ENZYMES ( 32 enzymes) #
###################################
AccI 1007 GTATAC GT^[AC][GT]AC
3785 GTAGAC
AcyI 1454 GACGCC G[GA]^CG[CT]C
1964 GACGTC
AlwNI 2478 CAGAATCTG CAG[ACGT][ACGT][ACGT]^CTG
3054 CAGCTGCTG
AseI 400 ATTAAT AT^TAAT
1979 ATTAAT
AsnI 400 ATTAAT AT^TAAT
1979 ATTAAT
BalI 180 TGGCCA TGG^CCA
3300 TGGCCA
BanI 361 GGCACC G^G[CT][GA]CC
2095 GGTGCC
BbvCI 3243 CCTCAGC CCTCAGC(-5/-2)
1997 GCTGAGG
BciVI 963 GTATCC GTATCC(6/5)
1800 GTATCC
BclI 1081 TGATCA T^GATCA
2428 TGATCA
BsaHI 1454 GACGCC G[GA]^CG[CT]C
1964 GACGTC
BsmI 718 GAATGC GAATGC(1/-1)
3179 GAATGC
CfoI 15 GCGC GCG^C
559 GCGC
DraII 820 AGGTCCT [GA]G^G[ACGT]CC[CT]
3254 AGGACCC
EaeI 180 TGGCCA [CT]^GGCC[GA]
3300 TGGCCA
EcoO109I 820 AGGTCCT [GA]G^G[ACGT]CC[CT]
3254 AGGACCC
EcoT22I 2271 ATGCAT ATGCA^T
2298 ATGCAT
FbaI 1081 TGATCA T^GATCA
2428 TGATCA
HhaI 15 GCGC GCG^C
559 GCGC
Hin1I 1454 GACGCC G[GA]^CG[CT]C
1964 GACGTC
HinP1I 15 GCGC G^CGC
559 GCGC
HpaI 977 GTTAAC GTT^AAC
2788 GTTAAC
MluNI 180 TGGCCA TGG^CCA
3300 TGGCCA
MscI 180 TGGCCA TGG^CCA
3300 TGGCCA
MspA1I 840 CAGCTG C[AC]G^C[GT]G
3054 CAGCTG
NsiI 2271 ATGCAT ATGCA^T
2298 ATGCAT
PpuMI 820 AGGTCCT [GA]G^G[AT]CC[CT]
3254 AGGACCC
PshBI 400 ATTAAT AT^TAAT
1979 ATTAAT
PvuII 840 CAGCTG CAG^CTG
3054 CAGCTG
SapI 674 GCTCTTC GCTCTTC(1/4)
3198 GCTCTTC
SmlI 1568 CTCAAG C^T[CT][GA]AG
3127 CTTAAG
XcmI 2116 CCATTTGAGAAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
3092 CCAGTAGAAAAATGG
#########################################
# 3 OR MORE CUT ENZYMES ( 78 enzymes) #
#########################################
AciI AcsI AfaI AflIII AluI
AlwI ApoI Asp700I AspHI AvaII
BbsI BbvI BfaI BglI BmyI
BpuAI BsaJI BseRI BsiHKAI BsiYI
BslI BsmAI BsmFI Bsp1286I BspHI
BspLU11I BsrI BssKI BstF5I BstNI
BstYI Cac8I Cfr13I DdeI DpnII
DraI EarI EcoRII EcoT14I Fnu4HI
FokI HaeIII HincII HindII HinfI
HphI ItaI Ksp632I MaeI MaeII
MboI MboII MflI MnlI MseI
MslI MvaI MwoI NdeII NlaIII
NlaIV NspI PleI RcaI RsaI
Sau3AI Sau96I ScrFI SfaNI SspI
StyI TfiI Tru9I TseI Tsp509I
TspRI XhoII XmnI