##################################
# NO CUT ENZYMES ( 67 enzymes) #
##################################
AatII Acc65I AccI AclI AhdI
Alw44I Aor51HI ApaLI AscI Asp718I
AspEI AspI BcgI BsaBI BsiWI
BsmI Bsp1407I BspDI BspLU11I BsrGI
BssHII Bst1107I BstBI BstEII BstPI
BstZ17I ClaI CpoI DraI DrdI
Eam1105I Eco47III EcoO65I EcoT22I FseI
KpnI KspI MamI NdeI NotI
NruI NsiI NspV PaeR7I PflFI
PmeI PshAI Psp1406I PvuI RsrII
SacII SalI SbfI ScaI SfiI
SfuI SgrAI SnaBI SpeI SphI
Sse8387I SspBI SspI SwaI Tth111I
XbaI XhoI
###################################
# ONE CUT ENZYMES ( 48 enzymes) #
###################################
AccIII 1935 TCCGGA T^CCGGA
AcsI 2941 GAATTC [GA]^AATT[CT]
AflII 3979 CTTAAG C^TTAAG
AgeI 1652 ACCGGT A^CCGGT
ApoI 2941 GAATTC [GA]^AATT[CT]
AseI 4215 ATTAAT AT^TAAT
AsnI 4215 ATTAAT AT^TAAT
Asp700I 1878 GAAGGTTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AviII 2509 TGCGCA TGC^GCA
AvrII 91 CCTAGG C^CTAGG
BamHI 950 GGATCC G^GATCC
BbrPI 785 CACGTG CAC^GTG
BbsI 3261 GAAGAC GAAGAC(2/6)
BclI 2535 TGATCA T^GATCA
BfrI 3979 CTTAAG C^TTAAG
BlnI 91 CCTAGG C^CTAGG
BlpI 695 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 695 GCTCAGC GC^T[ACGT]AGC
BpuAI 3261 GAAGAC GAAGAC(2/6)
BsaAI 785 CACGTG [CT]AC^GT[GA]
BsaI 3713 GGTCTC GGTCTC(1/5)
BseAI 1935 TCCGGA T^CCGGA
BspEI 1935 TCCGGA T^CCGGA
BspHI 821 TCATGA T^CATGA
BsrDI 1745 GCAATG GCAATG(2/0)
BssSI 2080 CACGAG CACGAG(-5/-1)
BstAPI 1278 GCAAGAACTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
CelII 695 GCTCAGC GC^T[ACGT]AGC
EcoRI 2941 GAATTC G^AATTC
EcoRV 1756 GATATC GAT^ATC
FbaI 2535 TGATCA T^GATCA
FspI 2509 TGCGCA TGC^GCA
MfeI 3695 CAATTG C^AATTG
MluI 550 ACGCGT A^CGCGT
MroI 1935 TCCGGA T^CCGGA
MunI 3695 CAATTG C^AATTG
NaeI 1459 GCCGGC GCC^GGC
NgoMIV 1459 GCCGGC G^CCGGC
NspI 4320 GCATGT [GA]CATG^[CT]
PacI 4212 TTAATTAA TTAAT^TAA
PinAI 1652 ACCGGT A^CCGGT
PmaCI 785 CACGTG CAC^GTG
PmlI 785 CACGTG CAC^GTG
PshBI 4215 ATTAAT AT^TAAT
RcaI 821 TCATGA T^CATGA
SapI 333 GCTCTTC GCTCTTC(1/4)
XcmI 1416 CCAGAAGGGAGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmnI 1878 GAAGGTTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 20 enzymes) #
###################################
AflIII 550 ACGCGT A^C[GA][CT]GT
786 ACGTGT
AocI 1761 CCTGAGG CC^T[ACGT]AGG
3209 CCTTAGG
BciVI 3043 GGATAC GTATCC(6/5)
3443 GTATCC
BglII 671 AGATCT A^GATCT
3232 AGATCT
BsaWI 1652 ACCGGT [AT]^CCGG[AT]
1935 TCCGGA
BsgI 274 CTGCAC GTGCAG(16/14)
1314 CTGCAC
BsiEI 180 CGGCCG CG[GA][CT]^CG
1114 CGGCCG
BsmBI 1475 GAGACG CGTCTC(1/5)
2322 GAGACG
Bsu36I 1761 CCTGAGG CC^T[ACGT]AGG
3209 CCTTAGG
EagI 180 CGGCCG C^GGCCG
1114 CGGCCG
EclXI 180 CGGCCG C^GGCCG
1114 CGGCCG
Eco52I 180 CGGCCG C^GGCCG
1114 CGGCCG
Eco81I 1761 CCTGAGG CC^T[ACGT]AGG
3209 CCTTAGG
HpaI 790 GTTAAC GTT^AAC
1582 GTTAAC
MaeII 786 ACGT A^CGT
2499 ACGT
NcoI 380 CCATGG C^CATGG
3448 CCATGG
NheI 735 GCTAGC G^CTAGC
1983 GCTAGC
PleI 3328 GACTC GAGTC(4/5)
4061 GAGTC
PvuII 767 CAGCTG CAG^CTG
3256 CAGCTG
SexAI 959 ACCAGGT A^CC[AT]GGT
2288 ACCAGGT
#########################################
# 3 OR MORE CUT ENZYMES ( 123 enzymes) #
#########################################
AccII AciI AcyI AfaI AluI
AlwI AlwNI ApaI AspHI AvaI
AvaII BalI BanI BanII BbeI
BbvCI BbvI BcnI BfaI BglI
BmyI BpmI BsaHI BsaJI BseRI
BsiHKAI BsiYI BslI BsmAI BsmFI
BsoBI Bsp1286I BspMI BsrBI BsrFI
BsrI BssKI BstF5I BstNI BstUI
BstXI BstYI Cac8I CfoI Cfr10I
Cfr13I DdeI DpnII DraII DraIII
DsaI EaeI EarI EcoNI EcoO109I
EcoRII EcoT14I FauI Fnu4HI FokI
HaeII HaeIII HapII HgaI HhaI
Hin1I HinP1I HincII HindII HindIII
HinfI HpaII HphI ItaI KasI
Ksp632I MaeI MaeIII MboI MboII
MflI MluNI MnlI MscI MseI
MslI MspA1I MspI MvaI MvnI
MwoI NarI NciI NdeII NlaIII
NlaIV PflMI PpuMI PstI RsaI
SacI Sau3AI Sau96I ScrFI SfaNI
SfcI SfoI SmaI SmlI StuI
StyI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I Van91I
XhoII XmaCI XmaI