##################################
# NO CUT ENZYMES ( 79 enzymes) #
##################################
AatII Acc65I AccIII AclI AcyI
AgeI AhdI AscI Asp700I Asp718I
AspEI AviII BbeI BbrPI BcgI
BglII BlpI Bpu1102I BsaHI BsaWI
BseAI BsiEI BspDI BspEI BspMI
BsrBI BsrFI BssHII BssSI BstBI
CelII Cfr10I ClaI CpoI EagI
Eam1105I EclXI Eco52I EcoRV FseI
FspI Hin1I HpaI KasI KpnI
KspI MfeI MluI MroI MunI
NaeI NarI NgoMIV NotI NruI
NspV PaeR7I PinAI PmaCI PmeI
PmlI PshAI Psp1406I PvuI RsrII
SacII SalI SexAI SfiI SfoI
SfuI SgrAI SmaI SwaI XbaI
XhoI XmaCI XmaI XmnI
###################################
# ONE CUT ENZYMES ( 37 enzymes) #
###################################
Alw44I 1490 GTGCAC G^TGCAC
ApaLI 1490 GTGCAC G^TGCAC
BanI 137 GGCACC G^G[CT][GA]CC
BciVI 1630 GGATAC GTATCC(6/5)
BclI 445 TGATCA T^GATCA
BglI 2162 GCCCCCCAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaAI 170 TACGTA [CT]AC^GT[GA]
BsaBI 3383 GATGATTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsmBI 4021 CGTCTC CGTCTC(1/5)
BstEII 3444 GGTCACC G^GT[ACGT]ACC
BstPI 3444 GGTCACC G^GT[ACGT]ACC
DraIII 1660 CACCGTGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 1534 GACTGTTTTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
DsaI 1972 CCATGG C^C[GA][CT]GG
EcoNI 3155 CCTTCCTGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 3444 GGTCACC G^GT[ACGT]ACC
EcoT22I 1281 ATGCAT ATGCA^T
FbaI 445 TGATCA T^GATCA
HincII 788 GTCAAC GT[CT]^[GA]AC
HindII 788 GTCAAC GT[CT]^[GA]AC
MamI 3383 GATGATTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
NcoI 1972 CCATGG C^CATGG
NdeI 3392 CATATG CA^TATG
NheI 2983 GCTAGC G^CTAGC
NsiI 1281 ATGCAT ATGCA^T
PacI 1859 TTAATTAA TTAAT^TAA
PleI 3182 GAGTC GAGTC(4/5)
PpuMI 2437 GGGTCCT [GA]G^G[AT]CC[CT]
SacI 1913 GAGCTC GAGCT^C
SapI 3665 GCTCTTC GCTCTTC(1/4)
SbfI 1072 CCTGCAGG CCTGCA^GG
SnaBI 170 TACGTA TAC^GTA
SpeI 4285 ACTAGT A^CTAGT
SphI 3896 GCATGC GCATG^C
Sse8387I 1072 CCTGCAGG CCTGCA^GG
SspI 4416 AATATT AAT^ATT
StuI 4319 AGGCCT AGG^CCT
###################################
# TWO CUT ENZYMES ( 43 enzymes) #
###################################
AccI 3542 GTATAC GT^[AC][GT]AC
4931 GTATAC
AccII 75 CGCG CG^CG
77 CGCG
AflII 725 CTTAAG C^TTAAG
4226 CTTAAG
AlwNI 684 CAGTCACTG CAG[ACGT][ACGT][ACGT]^CTG
2269 CAGTGGCTG
AocI 179 CCTGAGG CC^T[ACGT]AGG
3159 CCTGAGG
Aor51HI 867 AGCGCT AGC^GCT
2025 AGCGCT
ApaI 2000 GGGCCC GGGCC^C
2019 GGGCCC
AspI 2955 GACCGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
4016 GACCTCGTC
AvaI 53 CCCGAG C^[CT]CG[GA]G
3917 CTCGGG
AvrII 2059 CCTAGG C^CTAGG
2332 CCTAGG
BamHI 3677 GGATCC G^GATCC
3937 GGATCC
BfrI 725 CTTAAG C^TTAAG
4226 CTTAAG
BlnI 2059 CCTAGG C^CTAGG
2332 CCTAGG
BsaI 3825 GAGACC GGTCTC(1/5)
4754 GAGACC
BsiWI 168 CGTACG C^GTACG
172 CGTACG
BsmI 1279 GAATGC GAATGC(1/-1)
1764 GAATGC
BsoBI 53 CCCGAG C^[CT]CG[GA]G
3917 CTCGGG
Bsp1407I 4084 TGTACA T^GTACA
4667 TGTACA
BspHI 2507 TCATGA T^CATGA
4613 TCATGA
BspLU11I 3696 ACATGT A^CATGT
4206 ACATGT
BsrDI 1704 CATTGC GCAATG(2/0)
4363 CATTGC
BsrGI 4084 TGTACA T^GTACA
4667 TGTACA
Bst1107I 3542 GTATAC GTA^TAC
4931 GTATAC
BstAPI 1483 GCAGCTGGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
3067 GCACTCCCTGC
BstUI 75 CGCG CG^CG
77 CGCG
BstXI 3171 CCATTCCTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3775 CCAGCACTTTGG
BstZ17I 3542 GTATAC GTA^TAC
4931 GTATAC
Bsu36I 179 CCTGAGG CC^T[ACGT]AGG
3159 CCTGAGG
Eco47III 867 AGCGCT AGC^GCT
2025 AGCGCT
Eco81I 179 CCTGAGG CC^T[ACGT]AGG
3159 CCTGAGG
EcoRI 1514 GAATTC G^AATTC
4673 GAATTC
FauI 2202 CCCGC CCCGC(4/6)
3128 GCGGG
HaeII 867 AGCGCT [GA]GCGC^[CT]
2025 AGCGCT
HindIII 2280 AAGCTT A^AGCTT
3235 AAGCTT
MvnI 75 CGCG CG^CG
77 CGCG
PflFI 2955 GACCGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
4016 GACCTCGTC
PflMI 2268 CCAGTGGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2723 CCAGTCATTGG
PstI 1073 CTGCAG CTGCA^G
1339 CTGCAG
RcaI 2507 TCATGA T^CATGA
4613 TCATGA
ScaI 274 AGTACT AGT^ACT
1130 AGTACT
SspBI 4084 TGTACA T^GTACA
4667 TGTACA
Tth111I 2955 GACCGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
4016 GACCTCGTC
Van91I 2268 CCAGTGGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2723 CCAGTCATTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 99 enzymes) #
#########################################
AciI AcsI AfaI AflIII AluI
AlwI ApoI AseI AsnI AspHI
AvaII BalI BanII BbsI BbvCI
BbvI BcnI BfaI BmyI BpmI
BpuAI BsaJI BseRI BsgI BsiHKAI
BsiYI BslI BsmAI BsmFI Bsp1286I
BsrI BssKI BstF5I BstNI BstYI
Cac8I CfoI Cfr13I DdeI DpnII
DraI DraII EaeI EarI EcoO109I
EcoRII EcoT14I Fnu4HI FokI HaeIII
HapII HgaI HhaI HinP1I HinfI
HpaII HphI ItaI Ksp632I MaeI
MaeII MaeIII MboI MboII MflI
MluNI MnlI MscI MseI MslI
MspA1I MspI MvaI MwoI NciI
NdeII NlaIII NlaIV NspI PshBI
PvuII RsaI Sau3AI Sau96I ScrFI
SfaNI SfcI SmlI StyI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I XcmI XhoII