##################################
# NO CUT ENZYMES ( 70 enzymes) #
##################################
AatII Acc65I AccIII AclI AcyI
AgeI AhdI AscI Asp718I AspEI
BamHI BbeI BbrPI BglII BsaHI
BseAI BsiWI BspDI BspEI BssHII
Bst1107I BstEII BstPI BstZ17I ClaI
CpoI EagI Eam1105I EclXI Eco52I
EcoO65I FseI Hin1I HpaI KasI
KpnI KspI MfeI MluI MroI
MunI NarI NdeI NotI NruI
PacI PinAI PmaCI PmeI PmlI
Psp1406I PvuI RsrII SacII SalI
SapI SbfI SexAI SfiI SfoI
SgrAI SmaI SnaBI SpeI SphI
Sse8387I SspI SwaI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 50 enzymes) #
###################################
AccII 2732 CGCG CG^CG
AflII 4378 CTTAAG C^TTAAG
AflIII 2803 ACATGT A^C[GA][CT]GT
Alw44I 496 GTGCAC G^TGCAC
Aor51HI 3324 AGCGCT AGC^GCT
ApaI 4170 GGGCCC GGGCC^C
ApaLI 496 GTGCAC G^TGCAC
AseI 5647 ATTAAT AT^TAAT
AsnI 5647 ATTAAT AT^TAAT
AvaI 3161 CTCGAG C^[CT]CG[GA]G
AviII 2848 TGCGCA TGC^GCA
AvrII 5345 CCTAGG C^CTAGG
BalI 586 TGGCCA TGG^CCA
BanI 3581 GGCACC G^G[CT][GA]CC
BfrI 4378 CTTAAG C^TTAAG
BglI 1883 GCCTCCCCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlnI 5345 CCTAGG C^CTAGG
BsaBI 4512 GATAAATATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaWI 2742 TCCGGT [AT]^CCGG[AT]
BsiEI 1702 CGGTCG CG[GA][CT]^CG
BsmBI 1706 CGTCTC CGTCTC(1/5)
BsoBI 3161 CTCGAG C^[CT]CG[GA]G
Bsp1407I 4894 TGTACA T^GTACA
BspLU11I 2803 ACATGT A^CATGT
BsrGI 4894 TGTACA T^GTACA
BstAPI 2956 GCAGGATGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstBI 3272 TTCGAA TT^CGAA
BstUI 2732 CGCG CG^CG
DrdI 1698 GACACGGTCGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EaeI 586 TGGCCA [CT]^GGCC[GA]
Eco47III 3324 AGCGCT AGC^GCT
EcoRI 4160 GAATTC G^AATTC
EcoRV 3816 GATATC GAT^ATC
FspI 2848 TGCGCA TGC^GCA
HaeII 3324 AGCGCT [GA]GCGC^[CT]
HgaI 2733 GCGTC GACGC(5/10)
MamI 4512 GATAAATATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluNI 586 TGGCCA TGG^CCA
MscI 586 TGGCCA TGG^CCA
MvnI 2732 CGCG CG^CG
NcoI 1711 CCATGG C^CATGG
NheI 2826 GCTAGC G^CTAGC
NspV 3272 TTCGAA TT^CGAA
PaeR7I 3161 CTCGAG C^TCGAG
PflMI 696 CCACTTTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PshBI 5647 ATTAAT AT^TAAT
SfuI 3272 TTCGAA TT^CGAA
SspBI 4894 TGTACA T^GTACA
Van91I 696 CCACTTTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoI 3161 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 35 enzymes) #
###################################
AccI 4444 GTAGAC GT^[AC][GT]AC
5542 GTAGAC
AocI 920 CCTGAGG CC^T[ACGT]AGG
2154 CCTGAGG
AspI 1698 GACACGGTC GAC[ACGT]^[ACGT][ACGT]GTC
3874 GACTGAGTC
BcgI 3150 CGATCACTTTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
3163 CGAGGGAAGTGC
BlpI 2210 GCTGAGC GC^T[ACGT]AGC
4553 GCTAAGC
Bpu1102I 2210 GCTGAGC GC^T[ACGT]AGC
4553 GCTAAGC
BsaAI 1339 CACGTA [CT]AC^GT[GA]
4876 CACGTA
BspHI 2032 TCATGA T^CATGA
2813 TCATGA
BspMI 1615 ACCTGC ACCTGC(4/8)
3584 ACCTGC
BsrBI 4909 CCGCTC CCGCTC(-3/-3)
1856 GAGCGG
BsrDI 737 CATTGC GCAATG(2/0)
5639 CATTGC
BssSI 1232 CACGAG CACGAG(-5/-1)
3113 CTCGTG
BstYI 2622 AGATCC [GA]^GATC[CT]
4045 AGATCC
Bsu36I 920 CCTGAGG CC^T[ACGT]AGG
2154 CCTGAGG
CelII 2210 GCTGAGC GC^T[ACGT]AGC
4553 GCTAAGC
DraIII 697 CACTTTGTG CAC[ACGT][ACGT][ACGT]^GTG
1347 CACAGAGTG
DsaI 595 CCGTGG C^C[GA][CT]GG
1711 CCATGG
Eco81I 920 CCTGAGG CC^T[ACGT]AGG
2154 CCTGAGG
EcoT22I 100 ATGCAT ATGCA^T
3849 ATGCAT
FauI 662 CCCGC CCCGC(4/6)
1858 GCGGG
HincII 1590 GTCAAC GT[CT]^[GA]AC
2184 GTCAAC
HindII 1590 GTCAAC GT[CT]^[GA]AC
2184 GTCAAC
MflI 2622 AGATCC [GA]^GATC[CT]
4045 AGATCC
NaeI 2122 GCCGGC GCC^GGC
2556 GCCGGC
NgoMIV 2122 GCCGGC G^CCGGC
2556 GCCGGC
NsiI 100 ATGCAT ATGCA^T
3849 ATGCAT
PflFI 1698 GACACGGTC GAC[ACGT]^[ACGT][ACGT]GTC
3874 GACTGAGTC
PpuMI 1476 AGGTCCC [GA]G^G[AT]CC[CT]
3121 AGGACCC
PshAI 1471 GACAAAGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
2599 GACAGTCGTC
RcaI 2032 TCATGA T^CATGA
2813 TCATGA
ScaI 547 AGTACT AGT^ACT
3838 AGTACT
Tth111I 1698 GACACGGTC GAC[ACGT]^[ACGT][ACGT]GTC
3874 GACTGAGTC
XbaI 380 TCTAGA T^CTAGA
3770 TCTAGA
XcmI 1493 CCAGTCCAGAAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
4531 CCAAATAGTATGTGG
XhoII 2622 AGATCC [GA]^GATC[CT]
4045 AGATCC
#########################################
# 3 OR MORE CUT ENZYMES ( 103 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
AlwNI ApoI Asp700I AspHI AvaII
BanII BbsI BbvCI BbvI BciVI
BclI BcnI BfaI BmyI BpmI
BpuAI BsaI BsaJI BseRI BsgI
BsiHKAI BsiYI BslI BsmAI BsmFI
BsmI Bsp1286I BsrFI BsrI BssKI
BstF5I BstNI BstXI Cac8I CfoI
Cfr10I Cfr13I DdeI DpnII DraI
DraII EarI EcoNI EcoO109I EcoRII
EcoT14I FbaI Fnu4HI FokI HaeIII
HapII HhaI HinP1I HindIII HinfI
HpaII HphI ItaI Ksp632I MaeI
MaeII MaeIII MboI MboII MnlI
MseI MslI MspA1I MspI MvaI
MwoI NciI NdeII NlaIII NlaIV
NspI PleI PstI PvuII RsaI
SacI Sau3AI Sau96I ScrFI SfaNI
SfcI SmlI StuI StyI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I XmnI