##################################
# NO CUT ENZYMES ( 104 enzymes) #
##################################
AatII Acc65I AccII AccIII AclI
AcyI Aor51HI AscI Asp718I AspI
AviII AvrII BamHI BbeI BbrPI
BcgI BciVI BclI BglI BglII
BlnI BlpI Bpu1102I BsaAI BsaBI
BsaHI BseAI BsiWI BsmBI BspDI
BspEI BssHII BssSI Bst1107I BstEII
BstPI BstUI BstXI BstYI BstZ17I
CelII CfoI ClaI CpoI EagI
EclXI Eco47III Eco52I EcoNI EcoO65I
EcoRI EcoRV FbaI FseI FspI
HaeII HhaI Hin1I HinP1I KasI
KpnI KspI MamI MflI MluI
MroI MvnI NaeI NarI NdeI
NgoMIV NheI NotI NruI PacI
PflFI PflMI PmaCI PmeI PmlI
PshAI Psp1406I PvuI RsrII SacII
SalI SapI SbfI ScaI SexAI
SfiI SfoI SgrAI SmaI SnaBI
SphI Sse8387I SwaI Tth111I Van91I
XbaI XhoII XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 33 enzymes) #
###################################
AflII 4611 CTTAAG C^TTAAG
AgeI 211 ACCGGT A^CCGGT
AhdI 1729 GACCCCCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 2398 GTGCAC G^TGCAC
ApaI 2185 GGGCCC GGGCC^C
ApaLI 2398 GTGCAC G^TGCAC
AspEI 1729 GACCCCCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BalI 1823 TGGCCA TGG^CCA
BbvCI 1744 GCTGAGG CCTCAGC(-5/-2)
BcnI 1865 CCCGG CC^[GC]GG
BfrI 4611 CTTAAG C^TTAAG
BsaWI 211 ACCGGT [AT]^CCGG[AT]
BsiEI 387 CGACCG CG[GA][CT]^CG
BsrDI 2687 CATTGC GCAATG(2/0)
BstAPI 1474 GCAGCTGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstBI 3644 TTCGAA TT^CGAA
DrdI 2140 GACAATGTTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
DsaI 4406 CCATGG C^C[GA][CT]GG
Eam1105I 1729 GACCCCCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoT22I 4233 ATGCAT ATGCA^T
MluNI 1823 TGGCCA TGG^CCA
MscI 1823 TGGCCA TGG^CCA
NciI 1865 CCCGG CC^[GC]GG
NcoI 4406 CCATGG C^CATGG
NsiI 4233 ATGCAT ATGCA^T
NspV 3644 TTCGAA TT^CGAA
PaeR7I 863 CTCGAG C^TCGAG
PinAI 211 ACCGGT A^CCGGT
PpuMI 1001 AGGTCCT [GA]G^G[AT]CC[CT]
SacI 403 GAGCTC GAGCT^C
SfuI 3644 TTCGAA TT^CGAA
SpeI 4114 ACTAGT A^CTAGT
XhoI 863 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 27 enzymes) #
###################################
AccI 3297 GTAGAC GT^[AC][GT]AC
4262 GTCTAC
AlwNI 1475 CAGCTGCTG CAG[ACGT][ACGT][ACGT]^CTG
2601 CAGCTTCTG
AocI 1196 CCTTAGG CC^T[ACGT]AGG
4038 CCTAAGG
AseI 1561 ATTAAT AT^TAAT
3860 ATTAAT
AsnI 1561 ATTAAT AT^TAAT
3860 ATTAAT
AvaI 731 CCCGAG C^[CT]CG[GA]G
863 CTCGAG
BpmI 689 CTCCAG CTGGAG(16/14)
1331 CTCCAG
BsaI 1213 GAGACC GGTCTC(1/5)
3142 GGTCTC
BsgI 1876 CTGCAC GTGCAG(16/14)
2241 GTGCAG
BsoBI 731 CCCGAG C^[CT]CG[GA]G
863 CTCGAG
BspHI 4597 TCATGA T^CATGA
4790 TCATGA
BspLU11I 481 ACATGT A^CATGT
3638 ACATGT
BsrBI 876 CCGCTC CCGCTC(-3/-3)
1304 CCGCTC
BsrFI 211 ACCGGT [GA]^CCGG[CT]
389 ACCGGC
Bsu36I 1196 CCTTAGG CC^T[ACGT]AGG
4038 CCTAAGG
Cfr10I 211 ACCGGT [GA]^CCGG[CT]
389 ACCGGC
DraIII 1312 CACCCAGTG CAC[ACGT][ACGT][ACGT]^GTG
2581 CACACAGTG
EaeI 383 TGGCCG [CT]^GGCC[GA]
1823 TGGCCA
Eco81I 1196 CCTTAGG CC^T[ACGT]AGG
4038 CCTAAGG
HgaI 1390 GACGC GACGC(5/10)
4512 GACGC
HindIII 931 AAGCTT A^AGCTT
4723 AAGCTT
MaeII 1030 ACGT A^CGT
2177 ACGT
MfeI 245 CAATTG C^AATTG
2385 CAATTG
MunI 245 CAATTG C^AATTG
2385 CAATTG
PshBI 1561 ATTAAT AT^TAAT
3860 ATTAAT
RcaI 4597 TCATGA T^CATGA
4790 TCATGA
StuI 2033 AGGCCT AGG^CCT
4167 AGGCCT
#########################################
# 3 OR MORE CUT ENZYMES ( 94 enzymes) #
#########################################
AciI AcsI AfaI AflIII AluI
AlwI ApoI Asp700I AspHI AvaII
BanI BanII BbsI BbvI BfaI
BmyI BpuAI BsaJI BseRI BsiHKAI
BsiYI BslI BsmAI BsmFI BsmI
Bsp1286I Bsp1407I BspMI BsrGI BsrI
BssKI BstF5I BstNI Cac8I Cfr13I
DdeI DpnII DraI DraII EarI
EcoO109I EcoRII EcoT14I FauI Fnu4HI
FokI HaeIII HapII HincII HindII
HinfI HpaI HpaII HphI ItaI
Ksp632I MaeI MaeIII MboI MboII
MnlI MseI MslI MspA1I MspI
MvaI MwoI NdeII NlaIII NlaIV
NspI PleI PstI PvuII RsaI
Sau3AI Sau96I ScrFI SfaNI SfcI
SmlI SspBI SspI StyI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I XcmI XmnI