##################################
# NO CUT ENZYMES ( 60 enzymes) #
##################################
AatII AccI AccIII AflII AflIII
AgeI AscI AseI AsnI AvrII
BfrI BglII BlnI BsaBI BseAI
BsiWI BspDI BspEI BspLU11I BsrDI
Bst1107I BstBI BstEII BstPI BstZ17I
ClaI CpoI DraI EcoO65I EcoRV
EcoT22I FseI HindIII KspI MamI
MfeI MluI MroI MunI NdeI
NotI NruI NsiI NspV PacI
PinAI PmeI PshAI PshBI PvuI
RsrII SacII SalI ScaI SfiI
SfuI SgrAI SnaBI SwaI XbaI
###################################
# ONE CUT ENZYMES ( 40 enzymes) #
###################################
Acc65I 1421 GGTACC G^GTACC
AclI 3189 AACGTT AA^CGTT
AhdI 4788 GACAAGGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Asp700I 2251 GAAAGAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 1421 GGTACC G^GTACC
AspEI 4788 GACAAGGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AviII 275 TGCGCA TGC^GCA
BbrPI 271 CACGTG CAC^GTG
BcgI 3061 CGACGGCCATGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 1849 GGATAC GTATCC(6/5)
BsaAI 271 CACGTG [CT]AC^GT[GA]
BsmI 4996 GAATGC GAATGC(1/-1)
Bsp1407I 4781 TGTACA T^GTACA
BspHI 72 TCATGA T^CATGA
BsrGI 4781 TGTACA T^GTACA
BssHII 1225 GCGCGC G^CGCGC
DrdI 76 GACCTATGTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EagI 614 CGGCCG C^GGCCG
Eam1105I 4788 GACAAGGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EclXI 614 CGGCCG C^GGCCG
Eco52I 614 CGGCCG C^GGCCG
EcoRI 5109 GAATTC G^AATTC
FspI 275 TGCGCA TGC^GCA
HpaI 4250 GTTAAC GTT^AAC
KpnI 1421 GGTACC GGTAC^C
NheI 4038 GCTAGC G^CTAGC
PaeR7I 3513 CTCGAG C^TCGAG
PmaCI 271 CACGTG CAC^GTG
PmlI 271 CACGTG CAC^GTG
Psp1406I 3189 AACGTT AA^CGTT
RcaI 72 TCATGA T^CATGA
SapI 2759 GCTCTTC GCTCTTC(1/4)
SbfI 569 CCTGCAGG CCTGCA^GG
SpeI 1480 ACTAGT A^CTAGT
SphI 4192 GCATGC GCATG^C
Sse8387I 569 CCTGCAGG CCTGCA^GG
SspBI 4781 TGTACA T^GTACA
SspI 4681 AATATT AAT^ATT
XhoI 3513 CTCGAG C^TCGAG
XmnI 2251 GAAAGAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 16 enzymes) #
###################################
Alw44I 296 GTGCAC G^TGCAC
3155 GTGCAC
Aor51HI 309 AGCGCT AGC^GCT
1306 AGCGCT
ApaLI 296 GTGCAC G^TGCAC
3155 GTGCAC
BclI 2241 TGATCA T^GATCA
5431 TGATCA
BlpI 1309 GCTCAGC GC^T[ACGT]AGC
2684 GCTGAGC
Bpu1102I 1309 GCTCAGC GC^T[ACGT]AGC
2684 GCTGAGC
BssSI 1973 CACGAG CACGAG(-5/-1)
2381 CACGAG
CelII 1309 GCTCAGC GC^T[ACGT]AGC
2684 GCTGAGC
DraIII 299 CACTTGGTG CAC[ACGT][ACGT][ACGT]^GTG
3617 CACCTGGTG
Eco47III 309 AGCGCT AGC^GCT
1306 AGCGCT
FbaI 2241 TGATCA T^GATCA
5431 TGATCA
HincII 3783 GTTGAC GT[CT]^[GA]AC
4250 GTTAAC
HindII 3783 GTTGAC GT[CT]^[GA]AC
4250 GTTAAC
NaeI 1863 GCCGGC GCC^GGC
4553 GCCGGC
NgoMIV 1863 GCCGGC G^CCGGC
4553 GCCGGC
SexAI 771 ACCTGGT A^CC[AT]GGT
3618 ACCTGGT
#########################################
# 3 OR MORE CUT ENZYMES ( 142 enzymes) #
#########################################
AccII AciI AcsI AcyI AfaI
AluI AlwI AlwNI AocI ApaI
ApoI AspHI AspI AvaI AvaII
BalI BamHI BanI BanII BbeI
BbsI BbvCI BbvI BcnI BfaI
BglI BmyI BpmI BpuAI BsaHI
BsaI BsaJI BsaWI BseRI BsgI
BsiEI BsiHKAI BsiYI BslI BsmAI
BsmBI BsmFI BsoBI Bsp1286I BspMI
BsrBI BsrFI BsrI BssKI BstAPI
BstF5I BstNI BstUI BstXI BstYI
Bsu36I Cac8I CfoI Cfr10I Cfr13I
DdeI DpnII DraII DsaI EaeI
EarI Eco81I EcoNI EcoO109I EcoRII
EcoT14I FauI Fnu4HI FokI HaeII
HaeIII HapII HgaI HhaI Hin1I
HinP1I HinfI HpaII HphI ItaI
KasI Ksp632I MaeI MaeII MaeIII
MboI MboII MflI MluNI MnlI
MscI MseI MslI MspA1I MspI
MvaI MvnI MwoI NarI NciI
NcoI NdeII NlaIII NlaIV NspI
PflFI PflMI PleI PpuMI PstI
PvuII RsaI SacI Sau3AI Sau96I
ScrFI SfaNI SfcI SfoI SmaI
SmlI StuI StyI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
Tth111I TthHB8I Van91I XcmI XhoII
XmaCI XmaI