##################################
# NO CUT ENZYMES (   72 enzymes) #
##################################

AatII       Acc65I      AccI        AccIII      AclI        
AflII       AgeI        Aor51HI     AscI        AseI        
AsnI        Asp700I     Asp718I     AspI        BamHI       
BciVI       BfrI        BsaBI       BsaWI       BseAI       
BsiWI       BsmI        BspDI       BspEI       BspHI       
BspLU11I    Bst1107I    BstBI       BstZ17I     ClaI        
Eco47III    EcoRI       EcoRV       EcoT22I     FseI        
HincII      HindII      HindIII     HpaI        KpnI        
MamI        MfeI        MroI        MunI        NcoI        
NdeI        NotI        NruI        NsiI        NspV        
PacI        PflFI       PinAI       PmeI        PshAI       
PshBI       Psp1406I    PvuI        RcaI        SalI        
SbfI        ScaI        SexAI       SfuI        SgrAI       
SnaBI       SpeI        Sse8387I    SwaI        Tth111I     
XbaI        XmnI        


###################################
# ONE CUT ENZYMES (   35 enzymes) #
###################################

AcsI         4278  AAATTT               [GA]^AATT[CT]
AflIII       1721  ACGCGT               A^C[GA][CT]GT
AhdI          767  GACAAGAAGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
ApoI         4278  AAATTT               [GA]^AATT[CT]
AspEI         767  GACAAGAAGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AviII        2079  TGCGCA               TGC^GCA
BbrPI        3348  CACGTG               CAC^GTG
BbvCI         784  CCTCAGC              CCTCAGC(-5/-2)
BcgI         2352  GCATGAACATCG         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI         1677  TGATCA               T^GATCA
BglII        4218  AGATCT               A^GATCT
BsaAI        3348  CACGTG               [CT]AC^GT[GA]
BsgI         3501  CTGCAC               GTGCAG(16/14)
BsmBI        1915  GAGACG               CGTCTC(1/5)
BsrDI        2552  CATTGC               GCAATG(2/0)
BstAPI       3428  GCACCGTGTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII       1477  GGTGACC              G^GT[ACGT]ACC
BstPI        1477  GGTGACC              G^GT[ACGT]ACC
CpoI          169  CGGTCCG              CG^G[AT]CCG
DraI         3781  TTTAAA               TTT^AAA
DrdI         3289  GACCCCAAAGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eam1105I      767  GACAAGAAGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoNI        1729  CCTGGGGGAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I      1477  GGTGACC              G^GT[ACGT]ACC
FbaI         1677  TGATCA               T^GATCA
FspI         2079  TGCGCA               TGC^GCA
MluI         1721  ACGCGT               A^CGCGT
NheI         3233  GCTAGC               G^CTAGC
PaeR7I       1418  CTCGAG               C^TCGAG
PmaCI        3348  CACGTG               CAC^GTG
PmlI         3348  CACGTG               CAC^GTG
RsrII         169  CGGTCCG              CG^G[AT]CCG
SspI         4262  AATATT               AAT^ATT
StuI         4181  AGGCCT               AGG^CCT
XhoI         1418  CTCGAG               C^TCGAG


###################################
# TWO CUT ENZYMES (   20 enzymes) #
###################################

AlwI          781  GATCC                GGATC(4/5)
              325  GGATC               
AocI         1209  CCTCAGG              CC^T[ACGT]AGG
             4177  CCTGAGG             
AvrII        2242  CCTAGG               C^CTAGG
             3787  CCTAGG              
BbsI         1658  GAAGAC               GAAGAC(2/6)
             2918  GTCTTC              
BlnI         2242  CCTAGG               C^CTAGG
             3787  CCTAGG              
BpuAI        1658  GAAGAC               GAAGAC(2/6)
             2918  GTCTTC              
Bsp1407I      634  TGTACA               T^GTACA
             2548  TGTACA              
BsrGI         634  TGTACA               T^GTACA
             2548  TGTACA              
BssSI        2051  CACGAG               CACGAG(-5/-1)
             3083  CTCGTG              
BstYI         325  GGATCT               [GA]^GATC[CT]
             4218  AGATCT              
Bsu36I       1209  CCTCAGG              CC^T[ACGT]AGG
             4177  CCTGAGG             
DraIII       3429  CACCGTGTG            CAC[ACGT][ACGT][ACGT]^GTG
             3723  CACACTGTG           
Eco81I       1209  CCTCAGG              CC^T[ACGT]AGG
             4177  CCTGAGG             
MflI          325  GGATCT               [GA]^GATC[CT]
             4218  AGATCT              
SapI         1022  GAAGAGC              GCTCTTC(1/4)
             1804  GCTCTTC             
SfaNI        2235  GCATC                GCATC(5/9)
             3644  GCATC               
SfiI         2999  GGCCCTAGTGGCC        GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
             3595  GGCCCCAAGGGCC       
SphI         2717  GCATGC               GCATG^C
             3437  GCATGC              
SspBI         634  TGTACA               T^GTACA
             2548  TGTACA              
XhoII         325  GGATCT               [GA]^GATC[CT]
             4218  AGATCT              


#########################################
# 3 OR MORE CUT ENZYMES (  131 enzymes) #
#########################################

AccII       AciI        AcyI        AfaI        AluI        
Alw44I      AlwNI       ApaI        ApaLI       AspHI       
AvaI        AvaII       BalI        BanI        BanII       
BbeI        BbvI        BcnI        BfaI        BglI        
BlpI        BmyI        BpmI        Bpu1102I    BsaHI       
BsaI        BsaJI       BseRI       BsiEI       BsiHKAI     
BsiYI       BslI        BsmAI       BsmFI       BsoBI       
Bsp1286I    BspMI       BsrBI       BsrFI       BsrI        
BssHII      BssKI       BstF5I      BstNI       BstUI       
BstXI       Cac8I       CelII       CfoI        Cfr10I      
Cfr13I      DdeI        DpnII       DraII       DsaI        
EaeI        EagI        EarI        EclXI       Eco52I      
EcoO109I    EcoRII      EcoT14I     FauI        Fnu4HI      
FokI        HaeII       HaeIII      HapII       HgaI        
HhaI        Hin1I       HinP1I      HinfI       HpaII       
HphI        ItaI        KasI        Ksp632I     KspI        
MaeI        MaeII       MaeIII      MboI        MboII       
MluNI       MnlI        MscI        MseI        MslI        
MspA1I      MspI        MvaI        MvnI        MwoI        
NaeI        NarI        NciI        NdeII       NgoMIV      
NlaIII      NlaIV       NspI        PflMI       PleI        
PpuMI       PstI        PvuII       RsaI        SacI        
SacII       Sau3AI      Sau96I      ScrFI       SfcI        
SfoI        SmaI        SmlI        StyI        TaqI        
TfiI        Tru9I       TseI        Tsp45I      Tsp509I     
TspRI       TthHB8I     Van91I      XcmI        XmaCI       
XmaI