##################################
# NO CUT ENZYMES (   93 enzymes) #
##################################

AatII       AccI        AccIII      AclI        AcyI        
AhdI        Aor51HI     ApaI        AscI        AspEI       
AspI        AviII       BbeI        BbrPI       BbvCI       
BcgI        BclI        BglI        BsaAI       BsaBI       
BsaHI       BseAI       BsiEI       BsiWI       Bsp1407I    
BspDI       BspEI       BspMI       BsrBI       BsrGI       
BssSI       Bst1107I    BstBI       BstEII      BstPI       
BstXI       BstZ17I     ClaI        CpoI        DraIII      
DrdI        EagI        Eam1105I    EclXI       Eco47III    
Eco52I      EcoO65I     EcoT22I     FbaI        FseI        
FspI        HaeII       Hin1I       HpaI        KasI        
KspI        MaeII       MamI        MluI        MroI        
NaeI        NarI        NgoMIV      NheI        NotI        
NruI        NsiI        NspV        PacI        PaeR7I      
PflFI       PmaCI       PmeI        PmlI        Psp1406I    
PvuI        RsrII       SacI        SacII       SalI        
SbfI        ScaI        SexAI       SfiI        SfoI        
SfuI        SgrAI       SnaBI       SpeI        Sse8387I    
SspBI       Tth111I     XhoI        


###################################
# ONE CUT ENZYMES (   35 enzymes) #
###################################

AflIII       4127  ACATGT               A^C[GA][CT]GT
AgeI         3378  ACCGGT               A^CCGGT
Alw44I       2914  GTGCAC               G^TGCAC
AocI         3444  CCTAAGG              CC^T[ACGT]AGG
ApaLI        2914  GTGCAC               G^TGCAC
AseI         1855  ATTAAT               AT^TAAT
AsnI         1855  ATTAAT               AT^TAAT
AvrII        2721  CCTAGG               C^CTAGG
BamHI        1418  GGATCC               G^GATCC
BciVI        2697  GGATAC               GTATCC(6/5)
BglII        3099  AGATCT               A^GATCT
BlnI         2721  CCTAGG               C^CTAGG
BsaWI        3378  ACCGGT               [AT]^CCGG[AT]
BseRI        3356  CTCCTC               GAGGAG(10/8)
BsgI          228  GTGCAG               GTGCAG(16/14)
BsmBI         142  GAGACG               CGTCTC(1/5)
BspLU11I     4127  ACATGT               A^CATGT
BsrDI        3881  GCAATG               GCAATG(2/0)
BsrFI        3378  ACCGGT               [GA]^CCGG[CT]
BssHII        153  GCGCGC               G^CGCGC
Bsu36I       3444  CCTAAGG              CC^T[ACGT]AGG
Cfr10I       3378  ACCGGT               [GA]^CCGG[CT]
Eco81I       3444  CCTAAGG              CC^T[ACGT]AGG
EcoNI        2721  CCTAGGGGAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRV         814  GATATC               GAT^ATC
HgaI           26  GCGTC                GACGC(5/10)
PinAI        3378  ACCGGT               A^CCGGT
PshAI        1895  GACCACAGTC           GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PshBI        1855  ATTAAT               AT^TAAT
PstI         1610  CTGCAG               CTGCA^G
SapI          958  GAAGAGC              GCTCTTC(1/4)
SphI          556  GCATGC               GCATG^C
TaqI          347  TCGA                 T^CGA
TthHB8I       347  TCGA                 T^CGA
XcmI         3152  CCAACCAATTTTTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG


###################################
# TWO CUT ENZYMES (   35 enzymes) #
###################################

Acc65I       2789  GGTACC               G^GTACC
             3716  GGTACC              
AflII         580  CTTAAG               C^TTAAG
             3826  CTTAAG              
AlwNI         736  CAGCAGCTG            CAG[ACGT][ACGT][ACGT]^CTG
             2879  CAGTCCCTG           
Asp718I      2789  GGTACC               G^GTACC
             3716  GGTACC              
AspHI         386  GAGCAC               G[AT]GC[AT]^C
             2914  GTGCAC              
BalI          756  TGGCCA               TGG^CCA
             3968  TGGCCA              
BanII        1406  GGGCTC               G[GA]GC[CT]^C
             3338  GAGCCC              
BfrI          580  CTTAAG               C^TTAAG
             3826  CTTAAG              
BlpI          425  GCTGAGC              GC^T[ACGT]AGC
              859  GCTGAGC             
Bpu1102I      425  GCTGAGC              GC^T[ACGT]AGC
              859  GCTGAGC             
BsaI         2051  GAGACC               GGTCTC(1/5)
             4020  GGTCTC              
BsiHKAI       386  GAGCAC               G[AT]GC[AT]^C
             2914  GTGCAC              
BspHI         615  TCATGA               T^CATGA
              650  TCATGA              
BstAPI       1260  GCAGACTTTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
             2812  GCACCAGTTGC         
CelII         425  GCTGAGC              GC^T[ACGT]AGC
              859  GCTGAGC             
DsaI          804  CCATGG               C^C[GA][CT]GG
             1650  CCATGG              
EaeI          756  TGGCCA               [CT]^GGCC[GA]
             3968  TGGCCA              
EcoRI        1911  GAATTC               G^AATTC
             3866  GAATTC              
FauI          631  GCGGG                CCCGC(4/6)
              666  GCGGG               
HincII        913  GTCAAC               GT[CT]^[GA]AC
             1215  GTCAAC              
HindII        913  GTCAAC               GT[CT]^[GA]AC
             1215  GTCAAC              
HindIII       583  AAGCTT               A^AGCTT
             2893  AAGCTT              
KpnI         2789  GGTACC               GGTAC^C
             3716  GGTACC              
MfeI         2438  CAATTG               C^AATTG
             2779  CAATTG              
MluNI         756  TGGCCA               TGG^CCA
             3968  TGGCCA              
MscI          756  TGGCCA               TGG^CCA
             3968  TGGCCA              
MunI         2438  CAATTG               C^AATTG
             2779  CAATTG              
NcoI          804  CCATGG               C^CATGG
             1650  CCATGG              
NdeI         1802  CATATG               CA^TATG
             2534  CATATG              
PpuMI        1285  GGGTCCC              [GA]G^G[AT]CC[CT]
             3727  AGGACCT             
RcaI          615  TCATGA               T^CATGA
              650  TCATGA              
SfcI         1610  CTGCAG               C^T[GA][CT]AG
             3035  CTGTAG              
SspI         2425  AATATT               AAT^ATT
             3547  AATATT              
SwaI         3512  ATTTAAAT             ATTT^AAAT
             4241  ATTTAAAT            
XbaI         2047  TCTAGA               T^CTAGA
             2139  TCTAGA              


#########################################
# 3 OR MORE CUT ENZYMES (   95 enzymes) #
#########################################

AccII       AciI        AcsI        AfaI        AluI        
AlwI        ApoI        Asp700I     AvaI        AvaII       
BanI        BbsI        BbvI        BcnI        BfaI        
BmyI        BpmI        BpuAI       BsaJI       BsiYI       
BslI        BsmAI       BsmFI       BsmI        BsoBI       
Bsp1286I    BsrI        BssKI       BstF5I      BstNI       
BstUI       BstYI       Cac8I       CfoI        Cfr13I      
DdeI        DpnII       DraI        DraII       EarI        
EcoO109I    EcoRII      EcoT14I     Fnu4HI      FokI        
HaeIII      HapII       HhaI        HinP1I      HinfI       
HpaII       HphI        ItaI        Ksp632I     MaeI        
MaeIII      MboI        MboII       MflI        MnlI        
MseI        MslI        MspA1I      MspI        MvaI        
MvnI        MwoI        NciI        NdeII       NlaIII      
NlaIV       NspI        PflMI       PleI        PvuII       
RsaI        Sau3AI      Sau96I      ScrFI       SfaNI       
SmaI        SmlI        StuI        StyI        TfiI        
Tru9I       TseI        Tsp45I      Tsp509I     TspRI       
Van91I      XhoII       XmaCI       XmaI        XmnI