##################################
# NO CUT ENZYMES ( 86 enzymes) #
##################################
Acc65I AccI AccIII AclI AflII
AgeI AhdI Aor51HI AscI Asp718I
AspEI AspI AvaI AviII AvrII
BamHI BbeI BbrPI BciVI BfrI
BglI BlnI BsaAI BseAI BsiWI
BsoBI Bsp1407I BspEI BsrGI BssHII
Bst1107I BstEII BstPI BstXI BstZ17I
CpoI DraIII EagI Eam1105I EclXI
Eco47III Eco52I EcoO65I EcoRV EcoT22I
FseI FspI HpaI KasI KpnI
MfeI MluI MroI MunI NarI
NcoI NotI NruI NsiI PacI
PaeR7I PflFI PinAI PmaCI PmeI
PmlI PshAI Psp1406I PvuI RsrII
SacI SalI SbfI SfiI SfoI
SmaI SnaBI SphI Sse8387I SspBI
StuI SwaI Tth111I XhoI XmaCI
XmaI
###################################
# ONE CUT ENZYMES ( 36 enzymes) #
###################################
AatII 728 GACGTC GACGT^C
Alw44I 3963 GTGCAC G^TGCAC
ApaI 3096 GGGCCC GGGCC^C
ApaLI 3963 GTGCAC G^TGCAC
BanI 3545 GGCACC G^G[CT][GA]CC
BbvCI 2125 GCTGAGG CCTCAGC(-5/-2)
BcgI 324 GCACAGATGTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI 3978 TGATCA T^GATCA
BlpI 3239 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 3239 GCTGAGC GC^T[ACGT]AGC
BsiEI 22 CGACCG CG[GA][CT]^CG
BsmBI 1521 GAGACG CGTCTC(1/5)
BspDI 1451 ATCGAT AT^CGAT
BspHI 1065 TCATGA T^CATGA
BspLU11I 4280 ACATGT A^CATGT
BsrFI 17 GCCGGC [GA]^CCGG[CT]
BstAPI 2347 GCAGGTCATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
CelII 3239 GCTGAGC GC^T[ACGT]AGC
Cfr10I 17 GCCGGC [GA]^CCGG[CT]
ClaI 1451 ATCGAT AT^CGAT
DrdI 459 GACTCTTCAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoNI 3253 CCTGGAGAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
FbaI 3978 TGATCA T^GATCA
HaeII 3085 GGCGCT [GA]GCGC^[CT]
KspI 25 CCGCGG CCGC^GG
NaeI 17 GCCGGC GCC^GGC
NdeI 375 CATATG CA^TATG
NgoMIV 17 GCCGGC G^CCGGC
NheI 3227 GCTAGC G^CTAGC
NspI 4280 ACATGT [GA]CATG^[CT]
RcaI 1065 TCATGA T^CATGA
SacII 25 CCGCGG CCGC^GG
SgrAI 16 CGCCGGCG C[GA]^CCGG[CT]G
SpeI 4051 ACTAGT A^CTAGT
XbaI 2690 TCTAGA T^CTAGA
XcmI 2301 CCAAGCTTGAACTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
###################################
# TWO CUT ENZYMES ( 37 enzymes) #
###################################
AcyI 728 GACGTC G[GA]^CG[CT]C
3459 GACGCC
AflIII 544 ACGTGT A^C[GA][CT]GT
4280 ACATGT
AocI 1393 CCTCAGG CC^T[ACGT]AGG
4181 CCTGAGG
AseI 1166 ATTAAT AT^TAAT
4297 ATTAAT
AsnI 1166 ATTAAT AT^TAAT
4297 ATTAAT
Asp700I 1292 GAAAAACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2459 GAAGGCATTC
BalI 3102 TGGCCA TGG^CCA
3853 TGGCCA
BbsI 3911 GAAGAC GAAGAC(2/6)
4055 GTCTTC
BglII 553 AGATCT A^GATCT
3652 AGATCT
BpuAI 3911 GAAGAC GAAGAC(2/6)
4055 GTCTTC
BsaBI 1444 GATTGGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
3470 GATTTGCATC
BsaHI 728 GACGTC G[GA]^CG[CT]C
3459 GACGCC
BsaWI 699 ACCGGA [AT]^CCGG[AT]
860 ACCGGA
BsgI 849 GTGCAG GTGCAG(16/14)
2015 GTGCAG
BsmI 2463 GCATTC GAATGC(1/-1)
3933 GCATTC
BsrBI 1967 GAGCGG CCGCTC(-3/-3)
3092 GAGCGG
BssSI 2357 CTCGTG CACGAG(-5/-1)
2496 CTCGTG
BstBI 507 TTCGAA TT^CGAA
2552 TTCGAA
Bsu36I 1393 CCTCAGG CC^T[ACGT]AGG
4181 CCTGAGG
DsaI 25 CCGCGG C^C[GA][CT]GG
1533 CCACGG
Eco81I 1393 CCTCAGG CC^T[ACGT]AGG
4181 CCTGAGG
Hin1I 728 GACGTC G[GA]^CG[CT]C
3459 GACGCC
HindIII 498 AAGCTT A^AGCTT
2303 AAGCTT
MamI 1444 GATTGGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
3470 GATTTGCATC
MluNI 3102 TGGCCA TGG^CCA
3853 TGGCCA
MscI 3102 TGGCCA TGG^CCA
3853 TGGCCA
NspV 507 TTCGAA TT^CGAA
2552 TTCGAA
PflMI 483 CCACCAGATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1332 CCAAGGAATGG
PshBI 1166 ATTAAT AT^TAAT
4297 ATTAAT
PvuII 2969 CAGCTG CAG^CTG
3293 CAGCTG
SapI 2372 GAAGAGC GCTCTTC(1/4)
3164 GAAGAGC
ScaI 1115 AGTACT AGT^ACT
1469 AGTACT
SexAI 2601 ACCTGGT A^CC[AT]GGT
3402 ACCTGGT
SfuI 507 TTCGAA TT^CGAA
2552 TTCGAA
SspI 896 AATATT AAT^ATT
1367 AATATT
Van91I 483 CCACCAGATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1332 CCAAGGAATGG
XmnI 1292 GAAAAACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2459 GAAGGCATTC
#########################################
# 3 OR MORE CUT ENZYMES ( 99 enzymes) #
#########################################
AccII AciI AcsI AfaI AluI
AlwI AlwNI ApoI AspHI AvaII
BanII BbvI BcnI BfaI BmyI
BpmI BsaI BsaJI BseRI BsiHKAI
BsiYI BslI BsmAI BsmFI Bsp1286I
BspMI BsrDI BsrI BssKI BstF5I
BstNI BstUI BstYI Cac8I CfoI
Cfr13I DdeI DpnII DraI DraII
EaeI EarI EcoO109I EcoRI EcoRII
EcoT14I FauI Fnu4HI FokI HaeIII
HapII HgaI HhaI HinP1I HincII
HindII HinfI HpaII HphI ItaI
Ksp632I MaeI MaeII MaeIII MboI
MboII MflI MnlI MseI MslI
MspA1I MspI MvaI MvnI MwoI
NciI NdeII NlaIII NlaIV PleI
PpuMI PstI RsaI Sau3AI Sau96I
ScrFI SfaNI SfcI SmlI StyI
TaqI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI TthHB8I XhoII